Quantitative Analysis Using Stereology on Whole Slide Images
| Design-based stereology is a rigorous set of data analysis methods to ensure integrity in the quantitative analysis of the size, shape, and number of objects (usually cells) in a tissue sample. Stereology is essential to validating and rejecting experimental hypotheses and produces results that are unbiased, efficient, and more reliable than non-stereological quantitative analyses. Non-stereological cell and object detection methods are highly susceptible to sampling bias and often fail to account for differences in object size and distribution within a region of interest. Stereo Investigator Whole Slide Edition is a tool for analyzing whole slide image data accurately and efficiently. |  |
The Whole Slide version of Stereo Investigator is optimized for whole slide images and has a user interface that has been honed to be efficient and easy to learn.
Researchers use Stereo Investigator Whole Slide Edition to perform analysis of:
- cell population number
- fiber and capillary length
- area and volume of anatomical regions
- area and volume of cells
- area and volume fraction of Alzheimer plaques
Stereo Investigator Whole Slide Edition can analyze image data sets acquired from:
- BLiSS-200 and BLiSS-2000 slide scanners
- Vesalius resonant scanning confocal
- Zeiss
- Hamamatsu
- Leica
- Olympus
- Huron
- Aperio
- and more...
For more information about stereological methods and their importance in histology analysis go to http://www.stereology.info
Specially Designed Stereology Probes for Analyzing Whole Slide Images
There are numerous stereological probes included in Stereo Investigator Whole Slide edition for quantifying number, length, surface, volume, and area:
Number:
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Length:
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Surface:
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Volume/Area:
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Second order:
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Some examples are shown below:
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Quantify surface area per volume of gut smooth muscle layer with the Merz probe
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The length of dendrites in culture is analyzed with petrimetrics
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The percentage by volume of plaque in the cortex is analyzed with the area fraction fractionator probe
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Quantify the area of the lumen and the boundary-length of the internal elastic lamina of the cross section of an artery with the combined point-intercept probe
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Integrates with our Full Suite of Products
Get the most of whole slide images by scanning with the BLiSS-200 slide scanner, to storing and sharing using our image management platform Biolucida, to reconstructing with the 3D BrainMaker, and analyzing using Neurolucida 360 (for automatic 3D neuron reconstruction) and Stereo Investigator Whole Slide Edition.
Professional Technical Support
When you call us you will speak with a person - not an automated system. Talk to us about your hardware, software, or experimental design questions. Our team includes Ph.D. neuroscientists and experts in microscopy, stereology, neuron tracing, and image processing; ready to help you over the phone or online.
