Only Cellairus provides automatic, accurate, unbiased, high-throughput 3D cell quantification in fluorescently-labeled histological specimens.
Unbiased stereology is recognized as the gold standard for accurate quantification because it is a rigorous and accurate methodology for quantifying features of biological tissues. Cellairus dramatically accelerates stereological cell counting through the use of machine learning to replicate expert human observer judgments of about recognizing cells, their location and size. Once the machine learning algorithms are trained, Cellairus identifies cells in 3D image volumes throughout 3D brain regions using the same observer criteria as a human expert. Cellairus has been developed by the leading experts in stereology to preserve the integrity and benefits of stereology, while substantially reducing the amount of time needed to perform accurate quantification cell populations.
Cellairus uses state-of-the-art machine learning techniques, integrated with unbiased stereological counting rules, systematic random sampling (SRS) unbiased stereological sampling, and easy to learn workflows to classify, and quantify cells in 3D images. Cellairus automates the most widely used unbiased stereological probe for counting cells, the optical fractionator.
Using the defined counting frame, grid size and disector parameters, Cellairus detects all cells at each counting site. Once all objects are automatically detected within each counting frame site; the counting rules are then applied to filter and only retain the detected cells that fall within the disector.
Cellairus can be trained to differentiate between different cells types, sub-cellular objects and non-cell objects. To accommodate varying neuron densities in different brain regions, machine learning classifiers can be trained on both dense and sparse neuron populations. Entire studies are rapidly processed.
Download Cellairus product sheet here.
To avoid bias from standard image segmentation and thresholding techniques, we developed an intelligent automated parameter estimation process. This algorithm analyses individual subvolumes with high numbers of objects and optimizes key segmentation parameters to best match objects’ structure.
Objects are automatically detected in 3D within each counting frame site, the unbiased counting rules are then applied to only count the detected cells that fall within the optical disector. The well-established mathematical formulae used in design based stereology are then applied to produce statistically robust results about cell population that are assessed for accuracy using stereological coeffect of error estimates.
MBF products are used across the globe by the most prestigious laboratories.
MBF’s software utility is underscored by the number of references it receives in the worlds most important scientific publications.
Guerin, S. P., J. K. Melbourne, et al.
Astrocyte Reactivity and Neurodegeneration in the Female Rat Brain Following Alcohol DependenceView Publication
Wang, B., Y. Wang, et al.
The STING inhibitor C-176 attenuates MPTP-induced neuroinflammation and neurodegeneration in mouse parkinsonian modelsView Publication
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Human Spinal Oligodendrogenic Neural Progenitor Cells Enhance Pathophysiological Outcomes and Functional Recovery in a Clinically Relevant Cervical Spinal Cord Injury Rat ModelView Publication
Kasanga, E. A., Y. Han, et al.
Nigral-specific increase in ser31 phosphorylation compensates for tyrosine hydroxylase protein and nigrostriatal neuron loss: Implications for delaying parkinsonian signsView Publication
Muduli, N., S. Aparna, et al.
Saffron stigma extract and crocin play an important neuroprotective role in therapeutic measures against benzo[a]pyrene-induced behavioral alterations in zebrafishView Publication
Bazarek, S. F., M. Thaqi, et al.
Engineered neurogenesis in naïve adult rat cortex by Ngn2-mediated neuronal reprogramming of resident oligodendrocyte progenitor cellsView Publication
Kong, C., C. Pilger, et al.
Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber LaserView Publication
Cordeiro, L. M., M. V. Soares, et al.
Toxicity of Copper and Zinc alone and in combination in Caenorhabditis elegans model of Huntington's disease and protective effects of rutinView Publication
Lu, J., Behbahani, A.H., Hamburg, L. et al.
"Transforming representations of movement from body- to world-centric space."
Yes, it works with multichannel fluorescent images.
Yes, you are able to audit and inspect the results to assess the accuracy using the dynamic 3D visualization environment.
Yes, different classifiers can be trained for different types of cells or imaging methos. Cellairus is shipped with some classifiers built in. You can even contract training for your specific experiments to us and we will train the classifier for you.
Yes, the results will be computed using established stereological formulas.
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Andrew Hardaway, Ph.D. Vanderbuilt University
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Sigrid C. Veasey, MD University of Pennsylvania
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"We’ve been very happy for many years with MBF products and the course of upgrades and improvements. Your service department is outstanding. I have gotten great help from the staff with the software and hardware."
William E. Armstrong, Ph.D. University of Tennessee
"Our experience with the MBF equipment and especially the MBF people has been outstanding. I cannot speak any higher about their professionalism and attention for our needs."
Bogdan A. Stoica, MD University of Maryland
"MBF provides excellent technical support and helps you to find the best technical tools for your research challenges on morphometry."
Wilma Van De Berg, Ph.D. VU University Medical Center - Neuroscience Campus Amsterdam
Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron reconstruction, and image processing. We’ve also developed a host of additional support services, including:
At MBF, we’ve spent decades understanding the needs of researchers and their labs — and have a suite of products and solutions that have been specifically designed for the needs of today’s most important and advanced labs. Our commitment to you is to spend time with you discussing the needs of your lab — so that we can make sure the solutions we provide for you are exactly what you’ll need. It’s part of our commitment to supporting you — before, during, and after you’ve made your decision. We look forward to talking with you!