Only Cellairus provides automatic, accurate, unbiased, high-throughput 3D cell quantification in fluorescently-labeled histological specimens.
Unbiased stereology is recognized as the gold standard for accurate quantification because it is a rigorous and accurate methodology for quantifying features of biological tissues. Cellairus dramatically accelerates stereological cell counting through the use of machine learning to replicate expert human observer judgments of about recognizing cells, their location and size. Once the machine learning algorithms are trained, Cellairus identifies cells in 3D image volumes throughout 3D brain regions using the same observer criteria as a human expert. Cellairus has been developed by the leading experts in stereology to preserve the integrity and benefits of stereology, while substantially reducing the amount of time needed to perform accurate quantification cell populations.
Cellairus uses state-of-the-art machine learning techniques, integrated with unbiased stereological counting rules, systematic random sampling (SRS) unbiased stereological sampling, and easy to learn workflows to classify, and quantify cells in 3D images. Cellairus automates the most widely used unbiased stereological probe for counting cells, the optical fractionator.
Using the defined counting frame, grid size and disector parameters, Cellairus detects all cells at each counting site. Once all objects are automatically detected within each counting frame site; the counting rules are then applied to filter and only retain the detected cells that fall within the disector.
Cellairus can be trained to differentiate between different cells types, sub-cellular objects and non-cell objects. To accommodate varying neuron densities in different brain regions, machine learning classifiers can be trained on both dense and sparse neuron populations. Entire studies are rapidly processed.
Download product sheet here.
MBF products are used across the globe by the most prestigious laboratories.
MBF’s software utility is underscored by the number of references it receives in the worlds most important scientific publications.
Lu, J., Behbahani, A.H., Hamburg, L. et al.
"Transforming representations of movement from body- to world-centric space."
Uguz, I. and K. L. Shepard
“Spatially controlled, bipolar, cortical stimulation with high-capacitance, mechanically flexible subdural surface microelectrode arrays.” Sci AdvView Publication
Martin Molinero, G. D., G. G. Boldrini, et al.
“A soybean based-diet prevents Cadmium access to rat cerebellum, maintaining trace elements homeostasis and avoiding morphological alterations.” BioMetals.View Publication
Oddes, D., A. Ngwenya, et al.
“Orexinergic neurons in the hypothalami of an Asiatic lion, an African lion, and a Southeast African cheetah.” J Comp NeurolView Publication
Folorunso, O. O., T. L. Harvey, et al.
“The D-serine biosynthetic enzyme serine racemase is expressed by reactive astrocytes in the amygdala of human and a mouse model of Alzheimer’s disease.” Neuroscience LettersView Publication
Osorio, M. J., J. N. Mariani, et al.
“Glial progenitor cells of the adult human white and grey matter are contextually distinct.” GliaView Publication
Cui, X., Y.-Q. Weng, et al.
“The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons.” Brain and BehaviorView Publication
Yes, it works with multichannel fluorescent images.
Yes, you are able to audit and inspect the results to assess the accuracy using the dynamic 3D visualization environment.
Yes, different classifiers can be trained for different types of cells or imaging methos. Cellairus is shipped with some classifiers built in. You can even contract training for your specific experiments to us and we will train the classifier for you.
Yes, the results will be computed using established stereological formulas.
"I rarely have encountered a company so committed to support and troubleshooting as MBF."
"MBF Bioscience is extremely responsive to the needs of scientists and is genuinely interested in helping all of us in science do the best job we can."
"I am so happy to be a customer of your company. I always get great help related with your product or not. With the experienced members, you are the best team I've ever met. All of your staff are very kind and helpful. Thank you for your great help and support all the time."
"We’ve been very happy for many years with MBF products and the course of upgrades and improvements. Your service department is outstanding. I have gotten great help from the staff with the software and hardware."
"Our experience with the MBF equipment and especially the MBF people has been outstanding. I cannot speak any higher about their professionalism and attention for our needs."
"MBF provides excellent technical support and helps you to find the best technical tools for your research challenges on morphometry."
At MBF, we’ve spent decades understanding the needs of researchers and their labs — and have a suite of products and solutions that have been specifically designed for the needs of today’s most important and advanced labs. Our commitment to you is to spend time with you discussing the needs of your lab — so that we can make sure the solutions we provide for you are exactly what you’ll need. It’s part of our commitment to supporting you — before, during, and after you’ve made your decision. We look forward to talking with you!
Ground-breaking light sheet microscope system for cleared specimens.
The complete stereology solution. The gold standard for unbiased cell counting.
The gold standard in unbiased stereology for use on cleared tissue.
Unbiased stereology for whole slide images. Specially designed for analyzing large whole slide images.