Light Beads Microscopy

Fast volume imaging at 30Hz
MBF Bioscience > Light Beads Microscopy

Product Overview

The Light Beads Microscopy (LBM) unit is a groundbreaking add-on for a two-photon microscope, enabling the acquisition of a single volume with a depth of approximately 0.5mm at the same speed as capturing a single plane in a conventional microscope. This innovative module was developed by Dr. Alipasha Vaziri’s Laboratory of Neurotechnology and Biophysics at Rockefeller University in New York, NY.

Key Benefits

Current two-photon scanning microscopy techniques for brain imaging often face challenges in balancing image resolution, acquisition speed, and signal-to-noise ratio (SNR). These challenges become particularly pronounced during volumetric imaging due to the light-scattering properties of brain tissue.

Light Beads Microscopy (LBM) offers a solution by optimizing spatiotemporal signal acquisition to record data at rates limited by the fluorescence lifetime of neural calcium indicators, such as GCaMP. LBM leverages high-energy femtosecond laser pulses at a relatively low repetition rate (~4.5 MHz to 5 MHz) to enable the highly temporally multiplexed acquisition of up to 30 axial planes within the same timeframe required for traditional two-photon microscopy to capture a single plane. Additionally, LBM enhances SNR by utilizing one excitation pulse per voxel.

  • Wavelength (nm): 900 to 1050
  • Input polarization: vertical
  • Input beam size (mm): 3
  • Required laser frequency (MHz): 4.5 to 5
  • Input height (mm): 100
  • Dimensions (mm): 675x500x120


Laser Frequency (MHz) Maximum Number of Planes*
4.5 32
4.7 30
5 28

     *assuming a temporal window of 6.8ns for the demultiplexing channels

Rapid Volumetric Brain Imaging by Spatiotemporal Axial Beam Multiplexing

News About Light Beads Microscopy

Cited in Peer Reviewed Scientific Publications

MBF’s software utility is underscored by the number of references it receives in the worlds most important scientific publications.

Who Is Using MBF Products?

MBF products are used across the globe by the most prestigious laboratories. 


Robust Professional Support

Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron reconstruction, and image processing.  We’ve also developed a host of additional support services, including:


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