Light sheet microscopy represents a revolutionary breakthrough in biological imaging, enabling researchers to visualize entire organs and tissues in unprecedented detail. As a fluorescence imaging technique, light sheet microscopy utilizes a sheet of laser light to illuminate only a thin slice of the sample while detection occurs perpendicular to the illumination axis. This orthogonal arrangement provides intrinsic 3D optical sectioning, dramatically reducing photobleaching while capturing stunning 3D images of cleared tissue specimens.
Light sheet microscopy (also known as selective plane illumination microscopy or SPIM) is a cutting-edge imaging technique that uses a thin sheet of laser light to illuminate samples typically from the side, as with the SLICE light sheet microscope, or from specific theta angles from top, as with the ClearScope microscope, while detection occurs from above. The basic technical principle is a wide-field fluorescence microscope, placed perpendicular to the light-sheet, that collects the fluorescence signal through a full-frame camera.
This innovative approach offers several remarkable advantages for researchers studying cleared tissues:
Unlike confocal and spinning disc microscopy, light sheet microscopy’s orthogonal arrangement decouples illumination from detection, enabling:
SLICE represents a paradigm shift in delivering cutting-edge light sheet microscopy. This breakthrough system combines high performance with unprecedented affordability and compact design, making cutting-edge light sheet microscopy accessible to labs of all sizes:
ClearScope features patented light sheet theta microscopy technology with dual oblique illumination for imaging specimens of nearly any lateral size:
MBF Bioscience offers a comprehensive end-to-end light sheet microscopy workflow that allows you to analyze your intact sample in full and dive deep into the details:
Sample Preparation & Tissue Clearing
Transform your light sheet microscopy data into publication-ready insights with our specialized software suite designed specifically for light sheet applications.
Transform your light sheet microscopy data into quantifiable insights with our specialized analysis suite:
Neurolucida 360’s AI-powered tools automatically detect and trace neurons, spines, and vessels in your cleared tissue data, providing the gold standard in neuron reconstruction and analysis for light sheet microscopy applications.
NeuroInfo seamlessly aligns your cleared tissue data with standard brain atlases, automatically identifying key brain regions and quantifying cells or markers within each area. This transforms months of manual analysis into automated workflows that complete in hours.
Apply proven stereological principles to 3D light sheet datasets, ensuring unbiased quantification of cellular populations and structures throughout your cleared tissue samples.
Light sheet microscopy with SLICE systems transforms research across three key application areas:
Accelerate pharmaceutical research and drug development with light sheet microscopy systems designed for high-throughput screening and detailed tissue analysis.
Drug Discovery Benefits:
Light sheet microscopy has transformed neuroscience research by enabling comprehensive 3D analysis of neural structures and circuits across entire brain specimens. SLICE systems provide the gentle imaging capabilities essential for preserving delicate neural tissues while capturing unprecedented detail of neuronal architecture.
Key Neuroscience Benefits:
Light sheet microscopy has revolutionized cancer research by enabling detailed 3D visualization of tumor architecture and dynamics. SLICE enables researchers to image labeled cells and neuronal processes throughout cleared tissue with exceptional clarity and speed, making it suitable for diverse research applications across neuroscience, developmental biology, cancer research, and beyond.
Key Cancer Research Benefits:
Light sheet microscopy provides unprecedented insights into tissue regeneration and repair mechanisms through advanced 3D imaging capabilities.
Regenerative Medicine Advantages:
Ready to revolutionize your research with light sheet microscopy? Our team of scientists specializes in helping researchers determine the optimal combination of light sheet imaging systems and analysis software for specific applications.
Whether you’re new to light sheet microscopy or looking to upgrade your current imaging capabilities, we provide:
Every research question is unique. Our team of scientists is ready to help you determine the optimal combination of light sheet microscopy tools for your specific needs. Want to explore how light sheet microscopy and tissue clearing could advance your research? Contact us to discuss your specific applications and discover how light sheet microscopy can transform your discoveries.
Transform your biological imaging with state-of-the-art light sheet microscopy systems and analysis software. From benchtop SLICE systems to advanced ClearScope theta microscopes, we provide complete light sheet microscopy solutions for researchers worldwide.