Makes it easy to view, analyze, and share big image data from many sources
MBF Bioscience > MicroFile+

Product Overview

MicroFile+ is a free tool that solves a problem faced across laboratories and imaging facilities. Modern microscopic imaging devices can generate vast quantities and types of data. Organizing, storing, and analyzing this big data is a daunting problem that MicroFile+ solves. MicroFile+ turns large, unwieldy multi-dimensional data from most imaging sources into compact, easy to manage, and documented files that can be instantly loaded by MBF Bioscience products for analyses. The files can also be used by numerous other software applications. MicroFile+ also preserves instrumental and experimental metadata and includes extensive functionality for adding and curating metadata to fully describe and document image data.

Play Video

Key Benefits

MicroFile+ uses state-of-the-art compression methods to efficiently and accurately convert 2D and 3D images from most sources and formats into manageable, standardized experimental results. Formats from slide scanners, confocal microscopes, light sheet microscopes, and standard sources are all supported. Convert nearly any type of image to the widely used jpeg2000 or OME-TIFF standards. These formats efficiently store big image and metadata for fast retrieval, viewing, and analyses across applications. MicroFile+ can convert images without limits on the number of fluorescent and brightfield channels in the experiment.

MicroFile+ was developed with support by the SPARC program [Grant number 3OT3OD025349-01S2]

Compatible Input File Formats
  • JPEG2000 (.jp2)
  • JPEG (.jpg)
  • TIFF (.tif)
    • Generic TIFF
    • Vesalius TIFF
    • Flowview TIFF (Olympus)
    • OME TIFF
    • Keyence TIFF
    • Imaris TIFF
  • Zeiss CZI (.czi)
  • Leica LIF (.lif)
  • LSM
  • SVS/ Huron
  • CaliberID
  • Imaris IMS
  • ND2
  • NDPI

Download our product sheet here.

Who Is Using MicroFile+?

Microfile+ is used across the globe by the most prestigious laboratories. 

Cited in Peer Reviewed Scientific Publications

MBF’s software utility is underscored by the number of references it receives in the worlds most important scientific publications.

Frequently Asked Questions (FAQ)

What does this converter do?

The MicroFile+ converter identifies any recognizable metadata associated with files to pre-populate metadata fields. This minimizes the information that you need to input for converting image(s) to different image format(s). To obtain converted files that contain as much metadata as possible, we recommend that you convert files created using the default image-acquisition format for your imaging system.

What compression level is appropriate for my image data?

The appropriate compression level must be determined empirically. We suggest that you start testing with a 40:1 compression ratio. Open the images in your analysis software and compare with images that are not compressed. If there are visible artifacts, convert the source image again with a 20:1 compression ratio and compare to uncompressed image files. Repeat as needed, halving the compression ratio until there are no visible artifacts compared to uncompressed images.

We invite you to our forum for MicroFile+ software, where you can message experts and other users about topics like compression and other MBF Bioscience products.

Can deconvolved images be converted?

Any image in a compatible format can be converted. See specification section above for compatible file formats.

Is the data generated with MicroFile+ FAIR?

Yes. MicroFile+ supports the collaborative goals of Open Science through the practice of data openness, integrity, and reproducibility, by using MBF Bioscience’s published digital reconstruction data file format, the Neuromorphological File Specification (NFS), which was recently endorsed by the INCF. The data elements in this NFS format were specifically implemented to ensure the files are Findable, Accessible, Interoperable, and Reusable (FAIR).



Robust Professional Support

Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron reconstruction, and image processing.  We’ve also developed a host of additional support services, including:

  • Forums
    We have over 25 active forums where open discussions take place.
    >> Learn More
  • On-Site/Training
    We’ve conducted over 750 remote software installations.
    >> Learn More
  • Webinars
    We’ve created over 55 webinars that demonstrate our products & their uses.
    >> Learn More

Download MicroFile+

Please fill out the form below to download MicroFile+. An email with a download link and license code will be emailed to you.

Related Products

Neurolucida® 360

The most advanced software for automatic 3D neuron reconstruction.



Intelligent brain-wide cellular screening with anatomic specificity.


Vesselucida® 360

Automatically reconstruct microvascular networks in a 3D environment.



View, manage and share image stacks, 2D & 3D whole-slide images, and image stacks.