MicroFile+ is a free tool that solves a problem faced across laboratories and imaging facilities. Modern microscopic imaging devices can generate vast quantities and types of data. Organizing, storing, and analyzing this big data is a daunting problem that MicroFile+ solves. MicroFile+ turns large, unwieldy multi-dimensional data from most imaging sources into compact, easy to manage, and documented files that can be instantly loaded by MBF Bioscience products for analyses. The files can also be used by numerous other software applications. MicroFile+ also preserves instrumental and experimental metadata and includes extensive functionality for adding and curating metadata to fully describe and document image data.
MicroFile+ uses state-of-the-art compression methods to efficiently and accurately convert 2D and 3D images from most sources and formats into manageable, standardized experimental results. Formats from slide scanners, confocal microscopes, light sheet microscopes, and standard sources are all supported. Convert nearly any type of image to the widely used jpeg2000 or OME-TIFF standards. These formats efficiently store big image and metadata for fast retrieval, viewing, and analyses across applications. MicroFile+ can convert images without limits on the number of fluorescent and brightfield channels in the experiment.
|Compatible Input File Formats|
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Microfile+ is used across the globe by the most prestigious laboratories.
MBF’s software utility is underscored by the number of references it receives in the worlds most important scientific publications.
Lerchner, W., Adil, A.A., Mumuney, S. et al.
RNAi and chemogenetic reporter co-regulation in primate striatal interneurons.View Publication
Lane, A. R., I. C. Cogdell, et al.
"Genetic targeting of adult Renshaw cells using a Calbindin 1 destabilized Cre allele for intersection with Parvalbumin or Engrailed1." Scientific Reports 11(1): 19861.View Publication
Sheikh, A., X. Meng, et al.
"Neonatal Hypoxia-Ischemia Causes Persistent Intracortical Circuit Changes in Layer 4 of Rat Auditory Cortex." Cerebral Cortex(bhab365).View Publication
Prokop, S., P. Ábrányi-Balogh, et al.
"PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells." Nature Communications 12(1): 6505.View Publication
The MicroFile+ converter identifies any recognizable metadata associated with files to pre-populate metadata fields. This minimizes the information that you need to input for converting image(s) to different image format(s). To obtain converted files that contain as much metadata as possible, we recommend that you convert files created using the default image-acquisition format for your imaging system.
The appropriate compression level must be determined empirically. We suggest that you start testing with a 40:1 compression ratio. Open the images in your analysis software and compare with images that are not compressed. If there are visible artifacts, convert the source image again with a 20:1 compression ratio and compare to uncompressed image files. Repeat as needed, halving the compression ratio until there are no visible artifacts compared to uncompressed images.
We invite you to our forum for MicroFile+ software, where you can message experts and other users about topics like compression and other MBF Bioscience products.
Any image in a compatible format can be converted. See specification section above for compatible file formats.
Yes. MicroFile+ supports the collaborative goals of Open Science through the practice of data openness, integrity, and reproducibility, by using MBF Bioscience’s published digital reconstruction data file format, the Neuromorphological File Specification (NFS), which was recently endorsed by the INCF. The data elements in this NFS format were specifically implemented to ensure the files are Findable, Accessible, Interoperable, and Reusable (FAIR).
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"MBF provides excellent technical support and helps you to find the best technical tools for your research challenges on morphometry."
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