What is ScanImage software?
ScanImage is a state-of-the-art software product for controlling laser scanning microscopes. The application was first released in 2003 and the original version (r3.0) is described in .
ScanImage uses the powerful vDAQ hardware (described below) for microscope control. For legacy systems, National Instruments hardware is also supported. ScanImage runs on custom built microscopes and on commercial microscopes from Scientifica, Sutter and Thorlabs.
In an article titled “Biological imaging software tools” published in the journal, Nature Methods, ScanImage was described as follows : “ScanImage provides a software framework to control laser-scanning microscopes and is used extensively for two-photon excitation microscopy. It implements most standard modes of image acquisition and basic automation, and supports continuous image acquisition synchronized to behavioral or physiological data, which is particularly useful for imaging in intact animals. The software framework is object-oriented and event-driven to promote extensibility, online analysis and plug-in development. ScanImage can control laser-scanning microscopes, such as inhouse-built confocal systems, and allows for complex recordings where high signal-to-noise ratio is needed, such as tracking axon signaling in neuron cultures.”
 Pologruto TA, Sabatini BL, Svoboda K. ScanImage: flexible software for operating laser scanning microscopes. Biomed Eng Online 2003;2:13. doi: 10.1186/1475-925X-2-13.
 Eliceiri KW, Berthold MR, Goldberg IG, Ibáñez L, Manjunath BS, Martone ME, Murphy RF, Peng H, Plant AL, Roysam B, Stuurman N, Swedlow JR, Tomancak P, Carpenter AE. Biological imaging software tools. Nat Methods 2012;9(7):697-710. doi: 10.1038/nmeth.2084.