Unbiased stereology is recognized in biological research as the best-practice method for quantitative histology. It is used to accurately quantify the number of cells, the length of fibers, and the area and volume of biological structures or regions. Stereology plays an important role in validating and rejecting experimental hypotheses. It is often used to help answer questions such as:
Data and analyses obtained using stereology are more accurate than ad hoc quantitative analyses. Unbiased stereology uses systematic random sampling (a statistical sampling method) to select a representative sample of the region of interest. Then, researchers use a set of rigorously tested rules to mark cells and structures in each sampling site for quantification.
Learn more about quantifying cell populations and brain regions with stereology
By using unbiased stereology, researchers get unbiased, accurate and reliable data. For this reason, many peer-reviewed journals require or recommend the use of unbiased stereology when quantifying cells or structures. The Stereo Investigator system for unbiased stereology has been cited in over 8,000 published papers, making it by far the most-cited unbiased stereology system in the world.
In order to obtain the accurate results that unbiased stereology is known for, it is important to follow certain practices, described in the literature, during tissue preparation. MBF has Ph.D. staff scientists who can give you practical support for all aspects of stereological experimental design—from the tissue processing stage all the way to interpreting the results.
Additionally, MBF provides you with a host of expert stereological support that includes detailed software workflows within Stereo Investigator, extensive on-line documentation, videos and webinars. All of this is available to help guide you through the steps of a stereological study that are briefly outlined below.
>> Read our book about stereology (with a focus on Neuroscience)
>> View a powerpoint presentation on ‘Stereology Therory and Experimental Design’
A Stereo Investigator system provides you with accurate, unbiased estimates of the number, length, area, and volume of cells or biological structures in a tissue specimen. It is a key research tool that has led to advances in numerous areas of neuroscience including neurodegenerative diseases, neuropathy, memory, and behavior, as well as other research fields.
Stereo Investigator – Cleared Tissue Edition is the gold standard in unbiased stereology for use on cleared tissue. It allows researchers to use the world’s most cited stereology software — MBF Bioscience’s Stereo Investigator — to analyze intact, cleared tissue specimens imaged with light sheet or confocal microscopes.
Stereo Investigator – Whole Slide Edition is a specialized software application for analyzing whole slide image data accurately and efficiently.
At MBF, we’ve spent decades understanding the needs of researchers and their labs — and have a suite of products and solutions that have been specifically designed for the needs of today’s most important and advanced labs. Our commitment to you is to spend time with you discussing the needs of your lab — so that we can make sure the solutions we provide for you are exactly what you’ll need. It’s part of our commitment to supporting you — before, during, and after you’ve made your decision. We look forward to talking with you!