The SLAP2 is a revolutionary new microscope based on a technological breakthrough called second-generation Scanned Line Angular Projection two photon laser scanning microscopy that was recently developed by Dr. Kaspar Podgorski at the Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA.
Build your own state-of-the-art 2-photon microscope using the complete set of components and parts, including comprehensive instructions, supplied with the SLAP2 kit.
The game-changing innovation in the SLAP2 is the ability to perform unparalleled optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range (i.e., at 1000 Hz and higher) both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, neurotransmitter).
For the first time, this technology makes it possible to capture the dynamics of populations of neurons in vivo with millisecond temporal resolution and at least single-cell spatial resolution. This capability is critical for decoding how information is represented and processed by the billions of densely interconnected neurons comprising the mammalian CNS.
The neuroscience research implications of the SLAP2 are unprecedented. This novel microscope will enable groundbreaking optical imaging of neuronal activity in populations of neurons using fluorescent voltage indicators as well as fluorescent biosensors for neurotransmitters.
Kazemipour, A., Novak, O., Flickinger, D. et al. Author Correction: Kilohertz frame-rate two-photon tomography. Nat Methods 16, 932 (2019). https://doi.org/10.1038/s41592-019-0545-1
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MBF’s utility is underscored by the number of references it receives in the worlds most important scientific publications.
Lu, J., Behbahani, A.H., Hamburg, L. et al.
"Transforming representations of movement from body- to world-centric space."
Uguz, I. and K. L. Shepard
“Spatially controlled, bipolar, cortical stimulation with high-capacitance, mechanically flexible subdural surface microelectrode arrays.” Sci AdvView Publication
Martin Molinero, G. D., G. G. Boldrini, et al.
“A soybean based-diet prevents Cadmium access to rat cerebellum, maintaining trace elements homeostasis and avoiding morphological alterations.” BioMetals.View Publication
Oddes, D., A. Ngwenya, et al.
“Orexinergic neurons in the hypothalami of an Asiatic lion, an African lion, and a Southeast African cheetah.” J Comp NeurolView Publication
Folorunso, O. O., T. L. Harvey, et al.
“The D-serine biosynthetic enzyme serine racemase is expressed by reactive astrocytes in the amygdala of human and a mouse model of Alzheimer’s disease.” Neuroscience LettersView Publication
Osorio, M. J., J. N. Mariani, et al.
“Glial progenitor cells of the adult human white and grey matter are contextually distinct.” GliaView Publication
Cui, X., Y.-Q. Weng, et al.
“The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons.” Brain and BehaviorView Publication
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The MBF team has been highly receptive to the project needs and constantly strived to improve TissueMapper to enable smooth handling of large image stacks, in-depth annotation, and seamless mapping of external data sets onto a 3D map of the intrinsic cardiac nervous system. The all-around expertise of scientists and technologists at MBF was crucial to preprocess and assemble the large scale imaging data to jumpstart the 3D map building process. It is heartening to see MBF, a commercial entity, drive the development, standardization and dissemination of open data formats for complex anatomical annotation towards enabling friction-free exchange of these data sets in the scientific community.
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If you’re interested in building your own SLAP2 microscope, MBF Bioscience has everything you need! Contact us for more details.
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