The SLAP2 is a revolutionary new microscope based on a technological breakthrough called second-generation Scanned Line Angular Projection two photon laser scanning microscopy that was recently developed by Dr. Kaspar Podgorski (Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA).
The game-changing innovation in the SLAP2 is the ability to perform unparalleled optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range (i.e., at 1000 Hz and higher) both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, neurotransmitter).
For the first time, this technology makes it possible to capture the dynamics of populations of neurons in vivo with millisecond temporal resolution and at least single-cell spatial resolution. This capability is critical for decoding how information is represented and processed by the billions of densely interconnected neurons comprising the mammalian CNS.
The neuroscience research implications of the SLAP2 are unprecedented. This novel microscope will enable groundbreaking optical imaging of neuronal activity in populations of neurons using fluorescent voltage indicators as well as fluorescent biosensors for neurotransmitters.
Kazemipour, A., Novak, O., Flickinger, D. et al. Author Correction: Kilohertz frame-rate two-photon tomography. Nat Methods 16, 932 (2019). https://doi.org/10.1038/s41592-019-0545-1
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Lu, J., Behbahani, A.H., Hamburg, L. et al.
"Transforming representations of movement from body- to world-centric space."
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"A diencephalic circuit in rats for opioid analgesia but not positive reinforcement." Nature Communications 13(1): 764.View Publication
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"Interleukin-6 triggers toxic neuronal iron sequestration in response to pathological α-synuclein." Cell Reports 38(7): 110358.View Publication
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"Enhanced firing of locus coeruleus neurons and SK channel dysfunction are conserved in distinct models of prodromal Parkinson’s disease." Scientific Reports 12(1): 3180.View Publication
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"The Impacts of Early-life Adversity on Striatal and Hippocampal Memory Functions." Neuroscience.View Publication
Hibbard, E. A. and D. R. Sengelaub
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MBF Bioscience is now offering kits to researchers who wish to build their own SLAP2 microscopes. Contact us for more details.