SLAP2

A revolutionary 2-photon microscope for imaging neuronal activity at unprecedented speed
MBF Bioscience > SLAP2

Product Overview

The SLAP2 is a revolutionary new microscope based on a technological breakthrough called second-generation Scanned Line Angular Projection two photon laser scanning microscopy that was recently developed by Dr. Kaspar Podgorski (Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA).

glutamateimaging_v3
SLAP2 microscopy allows scientists to watch the activity of hundreds of synapses at a time in the brains of living animals. Here, red bursts mark synapses of a mouse neuron being excited by the neurotransmitter glutamate. Credit: Podgorski Lab

Key Benefits

The game-changing innovation in the SLAP2 is the ability to perform unparalleled optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range (i.e., at 1000 Hz and higher) both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, neurotransmitter).

For the first time, this technology makes it possible to capture the dynamics of populations of neurons in vivo with millisecond temporal resolution and at least single-cell spatial resolution. This capability is critical for decoding how information is represented and processed by the billions of densely interconnected neurons comprising the mammalian CNS.

The neuroscience research implications of the SLAP2 are unprecedented. This novel microscope will enable groundbreaking optical imaging of neuronal activity in populations of neurons using fluorescent voltage indicators as well as fluorescent biosensors for neurotransmitters.

Kazemipour, A., Novak, O., Flickinger, D. et al. Author Correction: Kilohertz frame-rate two-photon tomography. Nat Methods 16, 932 (2019). https://doi.org/10.1038/s41592-019-0545-1

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Cited in Peer Reviewed Scientific Publications

MBF’s utility is underscored by the number of references it receives in the worlds most important scientific publications.

Who Is Using Our Software?

MBF’s software is used across the globe by the most prestigious laboratories. 

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Robust Professional Support

Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron tracing, and image processing.  We’ve also developed a host of additional support services, including:

  • Forums
    We have over 25 active forums where open discussions take place.
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  • On-Site/Training
    We’ve conducted over 750 remote software installations.
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  • Webinars
    We’ve created over 55 webinars that demonstrate our products & their uses.
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Request more Information

MBF Bioscience is now offering kits to researchers who wish to build their own SLAP2 microscopes. Contact us for more details.