SLAP2 – Two Photon Microscope Kit
Kit for the revolutionary 2-photon microscope for imaging neuronal activity at unprecedented speedProduct Overview
The SLAP2 is a revolutionary new microscope based on a technological breakthrough called second-generation Scanned Line Angular Projection two photon laser scanning microscopy that was recently developed by Dr. Kaspar Podgorski at the Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA.
Build your own state-of-the-art 2-photon microscope using the complete set of components and parts, including comprehensive instructions, supplied with the SLAP2 kit.
Key Benefits
- Perform unparalleled optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range
- Temporal resolution in the millisecond range (1000 Hz and higher) both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, neurotransmitter).
- Capable of performing activity imaging at subcellular spatial resolution
The game-changing innovation in the SLAP2 is the ability to perform unparalleled optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range (i.e., at 1000 Hz and higher) both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, neurotransmitter).
For the first time, this technology makes it possible to capture the dynamics of populations of neurons in vivo with millisecond temporal resolution and at least single-cell spatial resolution. This capability is critical for decoding how information is represented and processed by the billions of densely interconnected neurons comprising the mammalian CNS.
The neuroscience research implications of the SLAP2 are unprecedented. This novel microscope will enable groundbreaking optical imaging of neuronal activity in populations of neurons using fluorescent voltage indicators as well as fluorescent biosensors for neurotransmitters.
SLAP2 microscope layout on an optical table. (A) Periscope. (B) Tier 1. (B1) Powell Lens. (B2) Polygonal Line Scanner. (B3) Surfboard 1. (B4) Surfboard 2. (C) Tier 2. (C1) Remote Focus 1. (C2) Remote Focus 2. (D) Front End. (D1) Objective lens and 2P Detection Path. (E) Platform for Sample. (F) Digital Micromirror Device, 1280x800 pixel array, pixel pitch 10.8μm (Image credit: Douglass M., Texas Instruments)
Download SLAP2 product sheet here.
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- Full field raster scan with the SLAP2 microscope. Image courtesy Dr. Kaspar Podgorski, HHMI
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- Visualization of 500 ROI scanned at 3.6KHZ
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- SLAP2
Scanned Line Angular Projection Two Photon Laser Scanning (SLAP2) Microscopy for Real-Time (Kilohertz Rates) Volumetric in Vivo Imaging at Subcellular Resolution
Cited in Peer Reviewed Scientific Publications
MBF’s utility is underscored by the number of references it receives in the worlds most important scientific publications.
Guerin, S. P., J. K. Melbourne, et al.
Astrocyte Reactivity and Neurodegeneration in the Female Rat Brain Following Alcohol DependenceView Publication
Wang, B., Y. Wang, et al.
The STING inhibitor C-176 attenuates MPTP-induced neuroinflammation and neurodegeneration in mouse parkinsonian modelsView Publication
Pieczonka, K., H. Nakashima, et al.
Human Spinal Oligodendrogenic Neural Progenitor Cells Enhance Pathophysiological Outcomes and Functional Recovery in a Clinically Relevant Cervical Spinal Cord Injury Rat ModelView Publication
Kasanga, E. A., Y. Han, et al.
Nigral-specific increase in ser31 phosphorylation compensates for tyrosine hydroxylase protein and nigrostriatal neuron loss: Implications for delaying parkinsonian signsView Publication
Muduli, N., S. Aparna, et al.
Saffron stigma extract and crocin play an important neuroprotective role in therapeutic measures against benzo[a]pyrene-induced behavioral alterations in zebrafishView Publication
Bazarek, S. F., M. Thaqi, et al.
Engineered neurogenesis in naïve adult rat cortex by Ngn2-mediated neuronal reprogramming of resident oligodendrocyte progenitor cellsView Publication
Kong, C., C. Pilger, et al.
Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber LaserView Publication
Cordeiro, L. M., M. V. Soares, et al.
Toxicity of Copper and Zinc alone and in combination in Caenorhabditis elegans model of Huntington's disease and protective effects of rutinView Publication
Lu, J., Behbahani, A.H., Hamburg, L. et al.
"Transforming representations of movement from body- to world-centric space."
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Robust Professional Support
Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron reconstruction, and image processing. We’ve also developed a host of additional support services, including:
- Forums
We have over 25 active forums where open discussions take place.
>> Learn More - On-Site/Training
We’ve conducted over 750 remote software installations.
>> Learn More - Webinars
We’ve created over 55 webinars that demonstrate our products & their uses.
>> Learn More
Request more Information
If you’re interested in building your own SLAP2 microscope, MBF Bioscience has everything you need! Contact us for more details.
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