SLAP2 – Two Photon Microscope Kit
Kit for the revolutionary 2-photon microscope for imaging neuronal activity at unprecedented speedProduct Overview
The SLAP2 is a revolutionary new microscope based on a technological breakthrough called second-generation Scanned Line Angular Projection two photon laser scanning microscopy that was recently developed by Dr. Kaspar Podgorski at the Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA.
Build your own state-of-the-art 2-photon microscope using the complete set of components and parts, including comprehensive instructions, supplied with the SLAP2 kit.
Key Benefits
- Perform unparalleled optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range
- Temporal resolution in the millisecond range (1000 Hz and higher) both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, neurotransmitter).
- Capable of performing activity imaging at subcellular spatial resolution
The game-changing innovation in the SLAP2 is the ability to perform unparalleled optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range (i.e., at 1000 Hz and higher) both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, neurotransmitter).
For the first time, this technology makes it possible to capture the dynamics of populations of neurons in vivo with millisecond temporal resolution and at least single-cell spatial resolution. This capability is critical for decoding how information is represented and processed by the billions of densely interconnected neurons comprising the mammalian CNS.
The neuroscience research implications of the SLAP2 are unprecedented. This novel microscope will enable groundbreaking optical imaging of neuronal activity in populations of neurons using fluorescent voltage indicators as well as fluorescent biosensors for neurotransmitters.
SLAP2 microscope layout on an optical table. (A) Periscope. (B) Tier 1. (B1) Powell Lens. (B2) Polygonal Line Scanner. (B3) Surfboard 1. (B4) Surfboard 2. (C) Tier 2. (C1) Remote Focus 1. (C2) Remote Focus 2. (D) Front End. (D1) Objective lens and 2P Detection Path. (E) Platform for Sample. (F) Digital Micromirror Device, 1280x800 pixel array, pixel pitch 10.8μm (Image credit: Douglass M., Texas Instruments)
Download SLAP2 product sheet here.
SLAP 2 Front View
SLAP2 Front View
SLAP2 Back View
SLAP 2 Back View
Full field raster scan with the SLAP2 microscope. Image courtesy Dr. Kaspar Podgorski, the Allen Institute
Visualization of 500 ROI scanned at 3.6KHZ
Scanned Line Angular Projection Two Photon Laser Scanning (SLAP2) Microscopy for Real-Time (Kilohertz Rates) Volumetric in Vivo Imaging at Subcellular Resolution
Recording Neurons’ Synaptic Input Patterns with Scanned Line Projection Microscopy
Cited in Peer Reviewed Scientific Publications
MBF’s utility is underscored by the number of references it receives in the worlds most important scientific publications.
Boeglin, M., E. Leyva-Díaz, et al.
Expression and function of C. elegans UNCP-18, a paralogue of the SM protein UNC-18View Publication
Rentsch, P., T. Egan, et al.
The ratio of M1 to M2 microglia in the striatum determines the severity of L-Dopa-induced dyskinesiasView Publication
Wang, Z., D. Zheng, et al.
Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic BrainView Publication
Villar-Conde, S., V. Astillero-Lopez, et al.
Synaptic involvement of the human amygdala in Parkinson’s diseaseView Publication
Stimpson, C. D., J. B. Smaers, et al.
Evolutionary scaling and cognitive correlates of primate frontal cortex microstructureView Publication
Russ, T., L. Enders, et al.
2,4-Dichlorophenoxyacetic Acid Induces Degeneration of mDA Neurons In VitroView Publication
Olkhova, E. A., C. Bradshaw, et al.
A novel mouse model of mitochondrial disease exhibits juvenile-onset severe neurological impairment due to parvalbumin cell mitochondrial dysfunctionView Publication
Zhang, X., C. Wang, et al.
Analysis of Error Sources in the Lissajous Scanning Trajectory Based on Two-Dimensional MEMS MirrorsView Publication
Lu, J., Behbahani, A.H., Hamburg, L. et al.
"Transforming representations of movement from body- to world-centric space."
Who Is Using MBF Products?
MBF products are used across the globe by the most prestigious laboratories.
Testimonials
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"MBF provides excellent technical support and helps you to find the best technical tools for your research challenges on morphometry."
Robust Professional Support
Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron reconstruction, and image processing. We’ve also developed a host of additional support services, including:
- Forums
We have over 25 active forums where open discussions take place.
>> Learn More - On-Site/Training
We’ve conducted over 750 remote software installations.
>> Learn More - Webinars
We’ve created over 55 webinars that demonstrate our products & their uses.
>> Learn More
Request more Information
If you’re interested in building your own SLAP2 microscope, MBF Bioscience has everything you need! Contact us for more details.
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