Yu CH, Stirman JN, Yu Y, Hira R, Smith SL.
A dual-engine two-photon mesoscope enabled high-resolution, simultaneous imaging across widely separated cortical regions. Independent scan control and adaptive optics supported flexible, large-scale recording of thousands of neurons in vivo, overcoming traditional field-of-view limitations in two-photon microscopy.... Read More
Pologruto TA, Sabatini BL, Svoboda K.
ScanImage enables real-time control of laser scanning microscopes using standard data acquisition hardware, eliminating the need for complex, custom electronics. It delivers high-quality, multi-channel imaging with microsecond-level precision while offering the flexibility researchers need to build and adapt advanced confocal and two-photon microscopy systems.... Read More
Pearre BW, Michas C, Tsang JM, Gardner TJ, Otchy TM.
A resonant-scanning two-photon microscope is used to enable high-speed direct laser writing, allowing accurate micron-scale 3D printing while maintaining sub-micron feature resolution.... Read More
Leiwe MN, Fujimoto S, Baba T, Moriyasu D, Saha B, Sakaguchi R, Inagaki S, Imai T.
An automated hue-based clustering approach overcomes limitations of conventional multicolour tracing in densely labeled tissue.... Read More
Zhang Y, Bizanti A, Harden SW, Chen J, Bendowski K, Hoover DB, Gozal D, Shivkumar K, Heal M, Tappan S, Cheng ZJ.
Whole-mount imaging and 3D reconstruction reveal the topographical organization of cardiac sympathetic axons.... Read More
Konopaske GT, Lange N, Coyle JT, Benes FM.
Both schizophrenia and bipolar disorder displayed decreased spine density and dendritic length in layer III pyramidal neurons of the DLPFC, with marked reductions in total spines per dendrite. These structural deficits point to overlapping synaptic abnormalities across the two disorders.... Read More
Merino-Galán L, Zamarbide M, Belloso-Iguerategui A, Alonso-Moreno MC, Gago B, Reinares-Sebastián A, Blesa J, Dumitriu D, Quiroga-Varela A, Rodríguez-Oroz MC.
Early dopamine depletion disrupts striatal plasticity but elicits compensatory spine and dendritic remodeling in SPNs, supporting network resilience in premotor parkinsonism.... Read More
Földy C, Malenka RC, Südhof TC.
Dendritic spine density declined in AD but remained intact in cognitively resilient cases, and network analysis of synaptic proteomes highlighted TWF2 as a key determinant of thin spine length. CRISPR-mediated TWF2 enhancement selectively extended thin spines without altering their density.... Read More
Scholler J, Jonsson J, Jordá-Siquier T, Gantar I, Batti L, Cheeseman BL, Pagès S, Sbalzarini IF, Lamy CM.
APR-native processing through pAPRica enables massive ClearScope datasets to be compressed, segmented and analyzed rapidly and accurately on standard hardware, supporting scalable whole-organ histopathology.... Read More
Dayton H, Kiss J, Wei M, Chauhan S, LaMarre E, Cornell WC, Morgan CJ, Janakiraman A, Min W, Tomer R, Price-Whelan A, Nirody JA, Dietrich LEP.
Aligned clonal architectures within P. aeruginosa biofilms define nutrient flow, metabolic zoning and antibiotic tolerance. Mutants with disrupted pili or O-antigen features lose this organization and exhibit heightened sensitivity to tobramycin.... Read More