Intelligent Brain-Wide Cellular Screening with Anatomic Specificity
NeuroInfo performs brain-wide analyses of cells and biochemical markers, and automatically categorizes them in neuroanatomical regions. Uses advanced deep learning methods - no more manually clicking cells or hand drawing anatomic boundaries.
Video abstract on The Journal of Comparative Neurology article "Automatic navigation system for the mouse brain" by Tappan et al.
Automatically Delineate Regions in the Brain
Automatically Register Histological Sections and Associated Data to the Allen Mouse Brain Atlas
With NeuroInfo, researchers can automatically identify and delineate brain regions within experimental mouse brain sections. NeuroInfo replaces the tedious manual process of neuroanatomical identification; no more paging through a reference atlas to match experimental images, and manually delineating anatomies in images. NeuroInfo integrates MBF’s renowned expertise in developing productive laboratory tools for neuroscientists, with the preeminent resource of the Allen Mouse Brain Atlas, allowing for accurate research with greater ease.
Introduction to: NeuroInfo
NeuroInfo assists researchers by enhancing their studies. Watch the video to see how fast, easy, and accurate our software is!
A rendering of a whole brain volume registered and compiled at full resolution by NeuroInfo from coronal mouse brains sections imaged at 10x. Images courtesy of Dr. Nuo Li and Dr. Alyse Thomas (Baylor College of Medicine) and Dr. Charles Gerfen (NIMH).
Mapping Neuroanatomical Structures Labeled in Histologic Sections to a Whole Brain Standardized Atlas Coordinate System
- Aligns whole slide images of experimental tissue sections to the Allen Brain Atlas
- Delineates anatomical regions in experimental sections
- Detects cells within the anatomical regions
- Aggregates measurements from within anatomical regions
- Maps all image measurements to anatomies in a standardized atlas space so that results can be compared across animals, experiments, and laboratories
- Support almost all file formats
Automatic Cell Detection and Mapping
Identify labeled neurons in whole slide images using an intuitive cell detection workflow. Our deep learning algorithms are robust against histologic and imaging artifacts such as edge effects, uneven illumination, and variable staining across specimens. Detected cells can be mapped to the Allen Mouse Brain Atlas and tallied in each of its 2500 brain structures, making comparative quantitative analysis between mice from different experiments and laboratories easy and standardized.
Why use NeuroInfo?
Identification and delineation of brain regions in histologic mouse brain sections is pivotal for many neurogenomics, transcriptomics, proteomics and connectomics studies. Yet, this process is usually manual and prone to observer error and bias. NeuroInfo automatically navigates an investigator through the complex microscopic anatomy of histologic sections of mouse brains, eliminating much of the user's potential for error.
NeuroInfo was Validated in a Recent Peer-Reviewed Publication
Extensive validation studies of identifying anatomies in NeuroInfo demonstrated that this novel technology performs remarkably well in accurately automatically delineating regions for fluorescent and brightfield microscopic imaging methods. A publication detailing this research and validation is available here:
Tappan, SJ, Eastwood, BS, O'Connor, N, et al. Automatic navigation system for the mouse brain. J Comp Neurol. 2019; 527: 2200– 2211. https://doi.org/10.1002/cne.24635
Eastwood, BS, Hooks, BM, Paletzki, RF, O'Connor, NJ, Glaser, JR, Gerfen, CR. Whole mouse brain reconstruction and registration to a reference atlas with standard histochemical processing of coronal sections. J Comp Neurol. 2019; 527: 2170– 2178. https://doi.org/10.1002/cne.24602
Hooks, B.M., Papale, A.E., Paletzki, R.F. et al. Topographic precision in sensory and motor corticostriatal projections varies across cell type and cortical area. Nat Commun 9, 3549 (2018) doi:10.1038/s41467-018-05780-7
NeuroInfo was developed in collaboration with the Allen Institute for Brain Science.
NeuroInfo Changes the Way We Look at the Nervous System
NeuroInfo yields repeatable, objective anatomic data that is invaluable for understanding the organization and composition of the nervous system, which will further knowledge in the fields of neurogenomics, transcriptomics, proteomics, and connectomics.
NeuroInfo's Atlas Calibration Tool
NeuroInfo’s unique calibration tool allows the registration of sections cut in any orientation (coronal, sagittal or horizontal). It also accomodates for differences in histological processing between laboratories so that it is applicable to the work performed in many laboratories.
Works with specimens imaged with:
Try it Out!
During your demonstration, you'll have the opportunity to talk to us about your hardware, software, or experimental design questions with our team of Ph.D. neuroscientists and experts in microscopy, neuron tracing, and image processing.
NeuroInfo has been developed with support from the National Institute of Mental Health's Small Business Innovation Research program
When you call us you will speak with a person - not an automated system. Talk to us about your hardware, software, or experimental design questions. Our team includes Ph.D. neuroscientists and experts in microscopy, stereology, neuron tracing, and image processing; ready to help you over the phone or online.
We can also custom develop for you
MBF is proud to help bioscience leaders and experts push the next wave of innovation and discovery to benefit humanity. Given this, it’s possible you’re looking for customized technology for your needs. If that is the case, click here to get in touch with us directly. We’d be happy and honored to see if we can create something together that will move all of us forward.