Neurolucida 360 Version 2020

Move at the Speed of Neuroscience with the Latest Version of Neurolucida 360


Does your image analysis software keep pace with your research?  We are excited to share that now–with the new version of Neurolucida 360 software that incorporates our novel approach to large data handling – ours can.

Every day neuroscientists around the globe contribute to the collective scientific knowledge base with new data, innovative ideas, and, occasionally, groundbreaking discoveries.  Innovations in microscopes, labeling reagents, and sample preparation techniques help fuel this progress; but these demand image-analysis software that can handle larger, more numerous and more complex data elements.

We’ve re-engineered image and data handling within MBF Bioscience software solutions from the ground up to maximize your computer hardware investment. No matter the image data size, from lightsheet microscopes, confocal microscopes, or slide scanners, you are now able to work efficiently, without delay. With our new intelligent image loading, maximized concurrent usage of CPU cores, multiple levels of data caching, and efficient use of RAM and GPU resources, you can now display and reconstruct your images faster than ever before to you make your scientific discoveries.



Image load times in Neurolucida 360 v.2020.3 are up to 5000x faster in the 3D environment, 250x faster in the 2D environment.


Our New Machine Learning Algorithms Allow you to Make New Discoveries


Puncta detection in the 3D environment allows you to detect anything from cells to punctate subcellular structures synapses. The feature includes the ability to utilize machine learning to generate more accurate reconstructions. The machine learning detection mechanism employs specific models of object size and appearance (e.g. for puncta, cell nuclei) that accept or reject locations in your image that meet general detection parameters. Only objects that meet the requirements of the specialized machine learning model are further considered for detection and reconstruction, leading to a more accurate selection of puncta of interest.

Our New High-Tech Imaging Engine also has New Tools Available Allows you to make your Scientific Discoveries Flow with Ease. These Include:




The subvolume function enables seamless, dynamic tracing on systematically selected focus sites of 2D and 3D image stacks within the 3D environment.




Color channel specification allows you to select one or multiple channels to view, hide, and reconstruct your images by associating traced structures with specific color channels in the 3D environment to facilitate multiplexed analyses.

Other New Features

  • Classify segments of trees in tree-editing mode to facilitate later analysis using Neurolucida Explorer software
  • A new image montage feature (optional) that considers image names in the 2D image-alignment process
  • An additional option in the right-click menu for selecting objects in Trace mode: Select everything of this contour type

Enhancements for SPARC Users

The Stimulating Peripheral Activity to Relieve Conditions (SPARC) program seeks to accelerate development of therapeutic devices that modulate electrical activity in nerves to improve organ function.

  • Network licensing is now available for SPARC researchers.
  • Improved Blackfynn Platform interface within Neurolucida 360 software, with updated file-transfer status indicators, icons, help links, and login.
  • SPARC Vocabulary Services launch when opening a file, rather than when starting Neurolucida 360 software.

Our File Format is Now Open and FAIR to Allow Collaboration Between the Scientific Community

To support the scientific community’s movement toward a more open and collaborative climate, we are making our digital reconstruction file format, the Neuromorpholocial File Specification open and available to all researchers. The structures represented have been continually developed for over 30 years, balancing accurate 3D representation of microscopic structures with the efficiency for reading, writing, storing, and rendering the reconstruction data. The neuromorphological file format balances structure with flexibility by storing each modeled object as a unique data element, and providing mechanisms for grouping any number and type of data elements. File-level metadata is retained to provide detail on the origin of the sample, ensuring that the provenance of derivative data is tracked and that important source information is not separated from the corresponding data. The decision to implement each neuromorphological structure, from spines to varicosities, was influenced by top neuroscientists who sought to maintain a meaningful model and have the necessary analysis options for that entity. The file specification has evolved with academia and discovery, and will continue to be updated as needed to define added or modified data elements.

Webinar: See the New Features of Neurolucida 360 2020.3.1

Join MBF Bioscience's Scientific Director, Dr. Susan Tappan, and Labs Analyst, TJ Tetreault, for a comprehensive demonstration of the latest capabilities of Neurolucida 360 and how they can benefit your research. They cover many new features such as puncta detection, generating and reconstructing within subvolumes, as well as the other vast computational improvements regarding image and data performance.


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How to get Neurolucida 360 Version 2020

If you have a current Software Upgrade and Support Subscription (SUSS), you can download version 2020 directly from our Download Center.

Download Version 2020

  • Don't know if your subscription is current? Contact us at 1-802-288-9290 or 
  • If your subscription is expired, contact us for a quote to renew your SUSS.

New to Neurolucida 360? We offer both a free evaluation and a free trial copy of Neurolucida 360.

Request a Free Trial

  1. Free evaluation: Submit some of your image data and let us evaluate how Neurolucida 360 can match your research experiment.
  2. Free 14-day trial copy: Download of a copy of Neurolucida 360 and use the tips and suggestions from the free evaluation to find out how easy it is to use and how quickly you can obtain useful data.

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