Neurolucida 360 Version History

MBF Bioscience > Product Resources  > Neurolucida 360 Resources > Neurolucida 360 Version History

Neurolucida 360 Version 2021.1.1 (Latest Version)

Released March 2021

New Features and Enhancements:

  • Added support for automatic z-alignment and using image names in the Image Montage tool
  • Improved puncta detection
  • Improved speed when tree segments are classified
  • Added ability to clip tracings on slices
  • Updated the Partition Contour window
  • Added more options for selecting markers, contours, or vessels with the new Freehand Selection tools
  • Updated tooltips that display when hovering on buttons and tools
  • Tracing with partial projection will automove the image through z
  • Updated the Illumination Correction tool
  • Updated how to access .blc files
  • Default image-save format is now .xml
  • Improved file support for the channel panel
  • Improved merge function for puncta editing

SPARC Users:

  • New Import 3D model tool to import and visualize generic organ scaffolds right in the 3D environment
  • Users can now keep the original color when linking to SciCrunch database
  • Added a LinkTerms exit button
  • Added ability to name sets with the SciCrunch term-list
  • Associate Link Terms to cell body contours
  • Increased the number of results from SciCrunch term-list searches
  • Associated terms are shown in the link terms window
  • Users can now load multiple term-lists into the term-verification window
  • New muscle fiber sugementation tool

Neurolucida 360 Version 2020.3.3

Released November 2020

New Features and Enhancements:

  • Added support for new AMD drivers
  • Additional Clean-Up functionality added to identify issues with varicosities
  • Decreased the time requires to detect puncta around the soma

Neurolucida 360 Version 2020.3.2

Released November 2020

New Features and Enhancements:

  • Added ability to select all dendrites, apical dendrites or axons in 3D environment
  • Reporting the thickness of a specific point in the 3D environment
  • Added progress bar for Clean-Up
  • Added support for additional OME-TIFF format

Neurolucida 360 Version 2020.3.1

Released October 2020

New Features and Enhancements:

  • Images and data files load noticeably faster than in previous releases
  • Neurolucida 360 software handles hundreds of thousands of data points simultaneously
  • Image adjustments are imediately displayed in both the 2D and 3D windows—without clicking any buttons
  • Multiple options to indicate how you want Neurolucida 360 software to handle and display your images
  • Drag and drop file opening is easier and more powerful with the new Image Opener window
    • Open all images in container files such as Leica ,lif and Zeiss ,czi formats
    • Choose to display in a grid, stack images, or display on top of each other without stacking
  • Puncta detection in the 3D environment replaces synapse detection present in previous releases
  • Puncta detection tool includes the option to use powerful new machine learning technology for accurate puncta detection
  • Color channel selection in the 3D environment via a new Channel panel enables you to select one or more color channels to view, hide, and associate data elements within your images
  • You can now associate traced structures with a specific color channel
  • New subvolume function in the 3D environment enables you to easily and systematically focus on portions of images/image stacks
  • Easier software authorization: Neurolucida 360 software autofills the online Authorization Request Form with information about your computer system
  • You can now classify segments of trees in tree-editing mode to facilitate later analysis using Neurolucida Explorer software
  • Research Resource Identifier (RRID) recorded in every .xml data file
  • Putative synapse markers work on all axon/dendrite pairs

Neurolucida 360 Version 2020.1.1

Released December 2019

New Features and Enhancements:

  • Image montage includes new method for 2D image alignment
  • Added ‘Select everything of this contour type’ option to the right-click menu
  • Add help access, improved statuses, icon, and login to Blackfynn access window
  • SPARC Vocabulary Services now launch when a data file or image file is opened, rather than when the software is started
  • Large Volume Reconstruction mode enhanced to adjust status warning

Neurolucida 360 Version 2019.1.3

Released October 2019

New Features and Enhancements:

  • SPARC vocabulary services: Can use symbols in Subject ID Field
  • Added ‘select everything of this contour type’ option to the right-click menu
 

Neurolucida 360 Version 2019.1.2

Released July 2019

Detection, Tracing, and Classification:

  • Batch tracing is CZI supported.
  • Input text box to filtering slider under parameters added to cell detection workflow.

Misc:

  • Batch converter added (DAT to XML) — to access, type “convert” in the quick launch box.

Neurolucida 360 Version 2019.1.1

Released January 2019

Detection, Tracing, and Classification:

  • End point of a trace changes colors when targeted when using user-guiding tracing system.
  • Automatic cell detection workflow added to speed up detection process.


Editing:

  • Nodes and points now distinguishable by color.
  • Added button to reset all selected contours/shells back to line mode display.


Scene and Display:

  • Completely redesigned preference panels with a handy search functionality.
  • Scalebar color and playment controls
  • New EMF detail slider.

Misc:

  • Display warning message when user attempts to change sections while moving to the next section.
  • Suppor for Huron jpeg files, definition for Huron file extension.
  • Support for Huron TIFF images.
  • LVR added to quick launch.
  • 3D contour drawing added.
  • 3D marker placement added.

Neurolucida 360 Version 2018.1.1

Released May 2018

Detection, Tracing, and Classification:

  • Display all trees as centerline while the tree being traced is displayed with the actual thickness.
  • Voxels in a single location cannot be identified as both a varicosity and a spine.
  • Varicosity detection is restricted to the varicosities whole center is located at least one radius away from the start or the end of a branch.
  • Improved automatic tracing in image volumes with numerous Z planes.
  • Reconstruct from a very large image volume and define subvolumes and trace in 3D.


Scene and Display:

  • Movie production has been redesigned and expanded functionality.


Misc:

  • Apply filters (Golgi, in vivo, closing, projection) to multiple images without opening them.
  • Function toggles between zooming and focusing depending on cursor location.

Neurolucida 360 Version 2017.01.3

Released October 2017

Detection, Tracing, and Classification:

  • Spine detection range increased from 15 to 20 micrometers.
  • Spine detector can use voxels past end of dendrite for interactive detection.
  • Surface area and otehr spine metrics can be calculated from outside the spine detector.

Neurolucida 360 Version 2017.01.2

Released September 2017

Detection, Tracing, and Classification:

  • In the trace ribbon, click Automatic>Batch Trace to generate the automatic reconstruction of somas, trees, and spines over multiple images.


Misc:

  • Marker deletion improved, more stable, faster with marker summary updated.
  • Contour functionality in 3D environment improved.

Neurolucida 360 Version 2017.01.1

Released April 2017

Detection, Tracing, and Classification:

  • Varicosities detection added (available with Neurolucida 360 Studio).
  • “Clear last” soma button added.
  • Synapse detection now available for axons, in addition to dendrites and apical dendrites.
  • Spine backbone length is reported by default.


Editing:

  • Cleanup tool added to identify and remove tree framents quickly after automatic tracing.
  • Marker sizing added to Edit Markers panel. Similar to 2D sizing (pixels, microns. or intrinsic).


Scene and Display:

  • 3D scale bar added.
  • 360 window renamed 3D environment.
  • Redesigned user interface with new icons.
  • When selecting an option and then selecting a different option, the display is updated.
  • Completely revamped user interface: ribbon bars replaced toolbars and menus.
  • Redesigned marker toolbar.


Misc:

  • Open data file in Neurolucida Exporer from the 3D environment with one click.
  • Objects can be grouped for analysis in Neurolucida Explorer.
  • Added option to save scaling information with image file and convert to .jpx format.
  • New version notification window displayed when opening program.
  • Apotome CZI, ND2, Calibar ID scanner, Bacus, Janelia, VSI, LSM files>4GB, tiled LSM, and multi channeled tiled LSM now supported.
  • Support for 4K monitors and Windows 10.
  • Support for saving and opening data files from Biolucida servers.
  • Improved handling of very large images.
  • Alert is displayed when dongle is not plugged in.
  • Context-sensitive help now available for users without internet access.