Microfile+ Makes It Easy to Convert, Compress, And Curate Image Data from Many Imaging Sources

MicroFile+ is a free tool that solves a problem faced across laboratories and imaging facilities. Modern microscopic imaging devices can generate vast quantities and types of data. Organizing, storing, and analyzing this big data is a daunting problem that MicroFile+ solves. MicroFile+ turns large, unwieldy multi-dimensional data from most imaging sources into compact, easy to manage, and documented files that can be instantly loaded by MBF Bioscience products for analyses. The files can also be used by numerous other software applications. MicroFile+ also preserves instrumental and experimental metadata and includes extensive functionality for adding and curating metadata to fully describe and document image data.


Highly effective for use with images produced by whole slide scanners. Compresses files for efficient storage and web streaming.   Optimized to work with multi-terabyte 3D images from lightsheet microscopes.


MicroFile+ uses state-of-the-art compression methods to efficiently and accurately convert 2D and 3D images from most sources and formats into manageable, standardized experimental results. Formats from slide scanners, confocal microscopes, light sheet microscopes, and standard sources are all supported. Convert nearly any type of image to the widely used jpeg2000 or OME-TIFF standards. These formats efficiently store big image and metadata for fast retrieval, viewing, and analyses across applications. MicroFile+ can convert images without limits on the number of fluorescent and brightfield channels in the experiment.


MicroFile+ is a free tool for Windows workstations that uses multi-threading to optimally utilize resources on computers ranging from standard to advanced multi-core configurations.


   I am excited about the tool and its impact on increasing the quality of the metadata associated with the image data. Can't wait to have the channels, scaling, and all other microscope settings incorporated in the image files. No more lost associated files, no more lost metadata.Thank you."

- Anna Pilko, Ph.D  
University of California San Diego

MicroFile+ Functionality

Direct Conversion

Convert nearly any type of image to the jpeg2000 or OME-TIFF standards. These formats efficiently store big image and metadata for fast retrieval, viewing, and analyses across applications. MicroFile+ supports: MBF JPEG2000, JPEG, generic TIFF, Vesalius/CaliberID TIFF, Flowview Olympus TIFF, OME TIFF, Keyence TIFF, Imaris TIFF, Zeiss LSM, Huron SVS, Leica SVS, CaliberID, HDF5, Imaris IMS, NDPI, DICOM, ND2, and LIF.


Volume Compilation

Combine individual images, such as serial sections or cleared tissue images, into 3D volumes.


Multichannel Conversion

MicroFile+ can handle complex data formats generated by lightsheet and fast scanning imaging platforms. Nearly any image format can be consolidated into accessible and understandable organization.

Visualization Projections

Produce popular data visualizations from series of images including deep focus, and maximum and minimum intensity projections on big data sets. 


Metadata Management

Curate metadata throughout your imaging workflow. MicroFile+ ensures that imaging parameters are maintained as datasets are organized, analyzed, and shared.






Process batches of images. MicroFile+ ensures workflow efficiency by cueing many datasets for conversion. 

Customer Reviews

"MBF provides excellent technical support and helps you to find the best technical tools for your research challenges on morphometry."
Wilma Van De Berg, Ph.D.
VU University Medical Center - Neuroscience Campus Amsterdam
"Our experience with the MBF equipment and especially the MBF people has been outstanding. I cannot speak any higher about their professionalism and attention for our needs."
Bogdan A. Stoica, M.D.
University of Maryland
"MBF Bioscience is extremely responsive to the needs of scientists and is genuinely interested in helping all of us in science do the best job we can."
Sigrid Veasey, M.D.
University of Pennsylvania

MicroFile+ Support

Get answers to your most pressing research and technical questions from MBF scientists and fellow researchers.


Visit the MBF Bioscience Forum


MicroFile+ Webinar


MBF Bioscience demonstrates how to use MicroFile+, our new and freely available desktop application, to convert microscopy image data from various proprietary file formats into the standardized OME-TIFF and JPEG2000 formats. 

Still Have Questions? Just Ask!

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