Neurolucida 360 v2.7: A minor release with major new features

algorithm 123.jpg-1

The 3 algorithms in Neurolucida 360 were used in combination
to create a smooth, accurate reconstruction

Minor releases typically don’t include new features, but Neurolucida 360 isn’t an ordinary piece of software. Neurolucida 360 v2.7 has many new features and improvements including:

  • A new automatic tracing algorithm – Rayburst Crawl
  • Capture videos of your rotating neuron reconstructions for presentations and publications
  • New backbone length analysis for dendritic spines
  • Improved handling of images exceeding 10GB

With the addition of Rayburst Crawl, Neurolucida 360 now has 3 different algorithms for automatic neuron reconstruction. Why 3 algorithms? To give you the power to choose the one that works best with your images. Labeling specificity, staining intensity, and image signal-to-noise can vary widely within a specimen – making it impossible for a single tracing algorithm to work optimally in all situations.

If you want more control over your neuron reconstructions, the same 3 algorithms can be used in user-guided mode. You follow a dendritic branch or axon with your mouse cursor and the algorithm finds the center and thickness of the process. It combines the unrivaled human ability to identify and segment objects with the speed of a computer.

Try it for yourself.

Request a free trial to see the algorithms in action

Or, if you are a customer with an up to date support subscription, download version 2.7

 

MBF Bioscience to Open European Headquarters in the Netherlands

Mbf Bioscience Europe

MBF Bioscience announced today that it will open a European Sales and Support office in Delft, the Netherlands. Masha Stern, who has been the Manager of Technical Support and Training at MBF Bioscience headquarters in Vermont for 11 years, will become the Managing Director of MBF Europe. The new office is scheduled to open September 2016.

“Our new European Office is a result of the continually increasing demand for our products in Europe,” says Jack Glaser, President of MBF Bioscience. “Our new office in the Netherlands will allow us to better serve our growing customer base in Europe. Masha Stern’s long-term experience in managing and supporting our products will provide our European customers with a source of significant expertise and dedicated support and sales information for our products.”

“I’m very excited to lead our European operations and to represent our award-winning technology in Europe,” said Masha.

Delft is home to the prestigious Delft University of Technology and is the birthplace of Antonie Philips van Leeuwenhoek, the “Father of Microbiology” and developer of high-quality, high magnification microscope lenses.

Contact Masha at MBF Bioscience Europe: masha@mbfbioscience.com.

 

About MBF Bioscience

MBF Bioscience helps life science researchers collect accurate, reliable data from tissue specimens. Founded in 1987 by the current president, Jack Glaser, and his father, Edmund Glaser, MBF Bioscience systems are used in over 1,000 labs worldwide and have been cited in more than 10,000 research papers. MBF Bioscience received a Tibbetts award from the U.S. Small Business Association in recognition of their contributions to technological innovation and service to federal research and development.

Researchers cited MBF systems in 22 papers during the week of 5/9/2016

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García-Lezana, T., Oria, M., Romero-Giménez, J., Bové, J., Vila, M., Genescà, J., . . . Cordoba, J. (2016). Cerebellar neurodegeneration in a new rat model of episodic hepatic encephalopathy. Journal of Cerebral Blood Flow and Metabolism. doi: 10.1177/0271678×16649196.

Gordon, R., Singh, N., Lawana, V., Ghosh, A., Harischandra, D. S., Jin, H., . . . Kanthasamy, A. (2016). Protein kinase Cδ upregulation in microglia drives neuroinflammatory responses and dopaminergic neurodegeneration in experimental models of Parkinson’s disease. Neurobiology of Disease. doi:http://dx.doi.org/10.1016/j.nbd.2016.04.008.

Huttenrauch, M., Brausz, A., Kurdakova, A., Borgers, H., Klinker, F., Liebetanz, D., . . . Wirths, O. (2016). Physical activity delays hippocampal neurodegeneration and rescues memory deficits in an Alzheimer disease mouse model. Transl Psychiatry, 6, e800. doi: 10.1038/tp.2016.65.

K.G, B., G, M.-N., & H, G. (2016). Early life stress accelerates behavioral and neural maturation of the hippocampus in male mice. Hormones and Behavior. doi: http://dx.doi.org/10.1016/j.yhbeh.2016.04.010.

Larpthaveesarp, A., Georgevits, M., Ferriero, D. M., & Gonzalez, F. F. (2016). Delayed erythropoietin therapy improves histological and behavioral outcomes after transient neonatal stroke. Neurobiology of Disease, 93, 57-63. doi: http://dx.doi.org/10.1016/j.nbd.2016.04.006.

Continue reading “Researchers cited MBF systems in 22 papers during the week of 5/9/2016” »

Researchers cited MBF systems in 10 papers during the week of 5/2/2016

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Aytan, N., Choi, J.-K., Carreras, I., Brinkmann, V., Kowall, N. W., Jenkins, B. G., & Dedeoglu, A. (2016). Fingolimod modulates multiple neuroinflammatory markers in a mouse model of Alzheimer’s disease. Scientific Reports, 6, 24939. doi: 10.1038/srep24939.

Helseth, A., Adil, S., Linabarger, M., Arehart, E., Fainberg, N., Singh, D., . . . Mikati, M. (2016). Novel Knock-in Mouse Model of Genetic Atp1a3 Dysfunction (S52. 006). Neurology, 86(16 Supplement), S52. 006.

Li, Y., Stockton, M. E., Bhuiyan, I., Eisinger, B. E., Gao, Y., Miller, J. L., . . . Zhao, X. (2016). MDM2 inhibition rescues neurogenic and cognitive deficits in a mouse model of fragile X syndrome. [10.1126/scitranslmed.aad9370]. Science translational medicine, 8(336), 336ra361-336ra361.

Lim, D. C., Brady, D. C., Soans, R., Kim, E. Y., Valverde, L., Keenan, B. T., . . . Pack, A. I. (2016). Different Cyclical Intermittent Hypoxia Severities have Different Effects on Hippocampal Microvasculature. [10.1152/japplphysiol.01040.2015]. Journal of Applied Physiology.

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Researchers cited MBF systems in 18 papers during the week of 4/25/2016

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Drzewiecki, C. M., Willing, J., & Juraska, J. M. (2016). Synaptic number changes in the medial prefrontal cortex across adolescence in male and female rats: A role for pubertal onset. Synapse, n/a-n/a. doi: 10.1002/syn.21909.

Inoue, T., Abe, C., Sung, S.-s. J., Moscalu, S., Jankowski, J., Huang, L., . . . Okusa, M. D. (2016). Vagus nerve stimulation mediates protection from kidney ischemia-reperfusion injury through α7nAChR+ splenocytes. The Journal of Clinical Investigation, 126(5). doi: 10.1172/jci83658.

Miller, A. N., Deal, D., Green, J., Houle, T., Brown, W., Thore, C., . . . Webb, L. X. (2016). Use of the Reamer/Irrigator/Aspirator Decreases Carotid and Cranial Embolic Events in a Canine Model. The Journal of Bone & Joint Surgery, 98(8), 658-664.

Ouda, L., Jílek, M., & Syka, J. (2016). Expression of c-Fos in rat auditory and limbic systems following 22-kHz calls. Behavioural Brain Research, 308, 196-204. doi: http://dx.doi.org/10.1016/j.bbr.2016.04.030.

Pantazopoulos, H., Wiseman, J. T., Markota, M., Ehrenfeld, L., & Berretta, S. (2016). Decreased numbers of somatostatin-expressing neurons in the amygdala of subjects with bipolar disorder or schizophrenia: Relationship to circadian rhythms. Biological Psychiatry. doi: http://dx.doi.org/10.1016/j.biopsych.2016.04.006.

Scheinert, R. B., Haeri, M. H., Lehmann, M. L., & Herkenham, M. (2016). Therapeutic effects of stress-programmed lymphocytes transferred to chronically stressed mice. Progress in Neuro-Psychopharmacology and Biological Psychiatry. doi: http://dx.doi.org/10.1016/j.pnpbp.2016.04.010.

Türer, A., Coşkun Türer, Ç., Durmuşlar, M. C., Balli, U., & Önger, M. E. (2016). The influence of oral administration of rosuvastatin on calvarial bone healing in rats. Journal of Cranio-Maxillofacial Surgery. doi:http://dx.doi.org/10.1016/j.jcms.2016.04.021. Continue reading “Researchers cited MBF systems in 18 papers during the week of 4/25/2016” »

MBF Bioscience Awarded Two Patents in 2016

InteractingWorms

We continue our history of innovation and invention as the U.S. Patent and Trademark Office awards us two patents. The first is for WormLab – our unique worm tracking software that gives researchers an enormous amount of behavioral data about a single worm or multiple worms, even as they go through omega bends, reversals, and entanglements. The second is for our radial shock wave technology that improves antibody penetration and reduces the time for antibody incubation and fixation periods.

WormLab is commercially available, while our shock wave technology is in development.

+ Learn more about WormLab

+ Read the WormLab patent

Read the shock wave patent

Researchers cited MBF systems in 10 papers during the week of 4/18/2016

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Darkazalli, A., Ismail, A. A. O., Abad, N., Grant, S. C., & Levenson, C. W. (2016). Use of human mesenchymal stem cell treatment to prevent anhedonia in a rat model of traumatic brain injury. Restorative Neurology and Neuroscience(Preprint), 1-9.

Deep, S. N., Baitharu, I., Sharma, A., Gurjar, A. K. S., Prasad, D., & Singh, S. B. (2016). Neuroprotective Role of L-NG-Nitroarginine Methyl Ester (L-NAME) against Chronic Hypobaric Hypoxia with Crowding Stress (CHC) Induced Depression-Like Behaviour.  Plos one, 11(4), e0153371. doi: 10.1371/journal.pone.0153371.

Delbary-Gossart, S., Lee, S., Baroni, M., Lamarche, I., Arnone, M., Canolle, B., . . . Beattie, M. S. (2016). A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury. Brain.

Gómez-López, S., Martínez-Silva, A. V., Montiel, T., Osorio-Gómez, D., Bermúdez-Rattoni, F., Massieu, L., & Escalante-Alcalde, D. (2016). Neural ablation of the PARK10 candidate Plpp3 leads to dopaminergic transmission deficits without neurodegeneration. Scientific Reports, 6, 24028. doi: 10.1038/srep24028

Haus, D. L., López-Velázquez, L., Gold, E. M., Cunningham, K. M., Perez, H., Anderson, A. J., & Cummings, B. J. (2016). Transplantation of human neural stem cells restores cognition in an immunodeficient rodent model of traumatic brain injury. Experimental Neurology. doi: http://dx.doi.org/10.1016/j.expneurol.2016.04.008.

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Researchers cited MBF systems in 20 papers during the week of 4/11/2016

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Baulch, J. E., Acharya, M. M., Allen, B. D., Ru, N., Chmielewski, N. N., Martirosian, V., . . . Limoli, C. L. (2016). Cranial grafting of stem cell-derived microvesicles improves cognition and reduces neuropathology in the irradiated brain. Proceedings of the National Academy of Sciences. doi: 10.1073/pnas.1521668113.

Ettle, B., Kerman, B. E., Valera, E., Gillmann, C., Schlachetzki, J. C. M., Reiprich, S., . . . Winkler, J. (2016). α-Synuclein-induced myelination deficit defines a novel interventional target for multiple system atrophy. Acta Neuropathologica, 1-17. doi: 10.1007/s00401-016-1572-y.

Hays, S. A., Ruiz, A., Bethea, T., Khodaparast, N., Carmel, J. B., Rennaker Ii, R. L., & Kilgard, M. P. (2016). Vagus Nerve Stimulation during Rehabilitative Training Enhances Recovery of Forelimb Function after Ischemic Stroke in Aged Rats. Neurobiology of Aging. doi:http://dx.doi.org/10.1016/j.neurobiolaging.2016.03.030.

Li, A., Roy, S. H., & Nattie, E. E. (2016). An augmented CO2 chemoreflex and overactive orexin system are linked with hypertension in young and adult spontaneously hypertensive rats (SHRs). The Journal of physiology, n/a-n/a. doi: 10.1113/jp272199.

Moro, N., Ghavim, S. S., Harris, N. G., Hovda, D. A., & Sutton, R. L. (2016). Pyruvate treatment attenuates cerebral metabolic depression and neuronal loss after experimental traumatic brain injury. Brain Research, 1642, 270-277. doi: http://dx.doi.org/10.1016/j.brainres.2016.04.005.

Continue reading “Researchers cited MBF systems in 20 papers during the week of 4/11/2016” »

Researchers cited MBF systems in 18 papers during the week of 4/4/2016

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a Dzaye, O. D., Hu, F., Derkow, K., Haage, V., Euskirchen, P., Harms, C., . . . Kettenmann, H. (2016). Glioma Stem Cells but Not Bulk Glioma Cells Upregulate IL-6 Secretion in Microglia/Brain Macrophages via Toll-like Receptor 4 Signaling. Journal of Neuropathology and Experimental Neurology.

Kadoya, K., Lu, P., Nguyen, K., Lee-Kubli, C., Kumamaru, H., Yao, L., . . . Tuszynski, M. H. (2016). Spinal cord reconstitution with homologous neural grafts enables robust corticospinal regeneration. Nature Medicine, advance online publication. doi: 10.1038/nm.4066

Kneynsberg, A., Collier, T. J., Manfredsson, F. P., & Kanaan, N. M. (2016). Quantitative and semi-quantitative measurements of axonal degeneration in tissue and primary neuron cultures. Journal of Neuroscience Methods, 266, 32-41. doi: http://dx.doi.org/10.1016/j.jneumeth.2016.03.004.

Köse, O., Arabacı, T., Gedikli, S., Eminoglu, D. Ö., Kermen, E., Kızıldağ, A., . . . Yemenoglu, H. (2016). Biochemical and histopathologic analysis of the effects of periodontitis on left ventricular heart tissues of rats. Journal of Periodontal Research, n/a-n/a. doi: 10.1111/jre.12380.

Mohanty, R., Das, S. k., Singh, N. R., & Patri, M. (2016). Withania somnifera Leaf Extract Ameliorates Benzo[a]pyrene-Induced Behavioral and Neuromorphological Alterations by Improving Brain Antioxidant Status in Zebrafish (Danio rerio). Zebrafish. doi: 10.1089/zeb.2015.1215.

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Iron Deficiency Worsens Fetal Alcohol Spectrum Disorders

An immunostained image of myelin basic protein in the cerebella of a mouse brain with an iron-sufficient diet compared with the brain of a mouse exposed to alcohol and fed an iron-insufficient diet. It shows the reduced cerebellar size due to the ID-alcohol combination. Green is MBP immunostain, blue is DAPI for nuclei.

An immunostained image of myelin basic protein in the cerebella of a mouse brain with an iron-sufficient diet compared with the brain of a mouse exposed to alcohol and fed an iron-insufficient diet. It shows the reduced cerebellar size due to the ID-alcohol combination. Green is MBP immunostain, blue is DAPI for nuclei. Image courtesy of Susan Smith, PhD.

If a pregnant woman drinks alcohol, she risks giving birth to a baby with physical and cognitive deficits – characteristics of fetal alcohol spectrum disorders. In a new study, researchers say that when the mother is low in iron, the consequences are even worse.

The scientists examined two groups of pregnant rats – one group was fed an iron sufficient diet while the other was fed a diet with insufficient iron levels. The offspring from both groups were exposed to alcohol from 4 to 9 days after birth – a time when their brains are going through a growth spurt and are particularly sensitive to alcohol. They were compared to offspring who received an iron-sufficient diet but were not exposed to alcohol. This growth spurt correlates to a growth spurt in humans that occurs during the third trimester of pregnancy.

The researchers used delay and trace eye blink classical conditioning methods to assess the offspring’s learning and memory. Learning impairments were reported in both alcohol-exposed groups regardless of their iron status, but more extreme impairments were seen in iron deficient rats compared to iron sufficient rats. After the behavioral tests were completed, the researchers studied the cerebellum and hippocampus – brain regions involved in learning and memory – at a cellular level.

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