Two 2014 Nobel Prize Laureates Used Neurolucida in their Groundbreaking Research

May-Britt and Edvard Moser

Drs. May-Britt and Edvard Moser Image from GEIR MOGEN / NTNU

Drs. May-Britt and Edvard Moser were awarded the 2014 Nobel Prize in Physiology or Medicine for discovering the cells that form a network for spatial navigation in the brain, and we’re proud to say they are MBF Bioscience customers and used Neurolucida in their research.

In 2006, the Norwegian husband and wife team published a paper in the journal Science entitled “Conjunctive Representation of Position, Direction, and Velocity in Entorhinal Cortex” – a pivotal step in a line of research initiated in 1971 by co-laureate Dr. John O’Keefe (The Hippocampus as a Spatial Map). In their study, the scientists used Neurolucida to create 3D reconstructions of a complex network of neurons that make it possible for rats, and other animals, including humans, to navigate the world around them.

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Researchers cited MBF systems in 16 papers during the week of 10/12/14

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Acker, S. N., Mandell, E. W., Sims-Lucas, S., Gien, J., Abman, S. H., & Galambos, C. (2014). Histologic Identification of Prominent Intrapulmonary Anastomotic Vessels in Severe Congenital Diaphragmatic Hernia. The Journal of Pediatrics(0).

Dixon, K. J., Theus, M. H., Nelersa, C. M., Mier, J., Travieso, L. G., Yu, T.-S., . . . Liebl, D. J. (2014). Endogenous neural stem/progenitor cells stabilize the cortical microenvironment following traumatic brain injury. Journal of Neurotrauma.

Janelidze, S., Nordström, U., Kügler, S., & Brundin, P. (2014). Pre‐existing immunity to AAV2 limits transgene expression following intracerebral AAV2‐based gene delivery in a 6‐OHDA model of Parkinson’s disease. The Journal of Gene Medicine.

Le Belle, Janel E., Sperry, J., Ngo, A., Ghochani, Y., Laks, D. R., López-Aranda, M., . . . Kornblum, Harley I. (2014). Maternal Inflammation Contributes to Brain Overgrowth and Autism-Associated Behaviors through Altered Redox Signaling in Stem and Progenitor Cells. Stem Cell Reports(0).

Polinski, N. K., Gombash, S. E., Manfredsson, F. P., Lipton, J. W., Kemp, C. J., Cole-Strauss, A., . . . Sortwell, C. E. (2014). Recombinant adeno-associated virus 2/5-mediated gene transfer is reduced in the aged rat midbrain. Neurobiology of Aging. doi: 10.1016/j.neurobiolaging.2014.07.047

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Anorexia Accelerates the Development of the Rat Hippocampus

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This image stack was used in the study to analyze spine density. Image courtesy of Tara Chowdhury, Ph.D. first author of the study.

To find out how anorexia nervosa changes the brain, scientists at New York University are studying a rat model of the disease called activity-based anorexia (ABA). Previously, they discovered that ABA rats develop unusually robust dendritic branching of neurons in part of the hippocampus. Their new study takes those findings a step further, illuminating more differences between the brains of healthy versus ABA rats, and offering evidence that ABA rats may be developing too early, closing a critical period of development too soon.

But before making any conclusions about ABA brains, the researchers made some interesting discoveries about normal brain development. Using Neurolucida to analyze CA1 pyramidal cells in the stratum radiatum layer of the ventral hippocampus, they found that after puberty, around postnatal day 51, dendrites go through a growth spurt, more than doubling the number of branches seen seven days earlier. This growth spurt is followed by a decrease, or a pruning, which the researchers say is part of the normal maturation process.

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Researchers cited MBF systems in 15 papers during the week of 10/05/14

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Boada, M. D., Gutierrez, S., Aschenbrenner, C. A., Houle, T. T., Hayashida, K.-i., Ririe, D. G., & Eisenach, J. C. (2014). Nerve injury induces a new profile of tactile and mechanical nociceptor input from undamaged peripheral afferents. Journal of Neurophysiology.

Hammels, C., Prickaerts, J., Kenis, G., Vanmierlo, T., Fischer, M., Steinbusch, H. W. M., . . . Rutten, B. P. F. (2014). Differential susceptibility to chronic social defeat stress relates to the number of Dnmt3a-immunoreactive neurons in the hippocampal dentate gyrus. Psychoneuroendocrinology(0).

Nordström, U., Beauvais, G., Ghosh, A., Pulikkaparambil Sasidharan, B. C., Lundblad, M., Fuchs, J., . . . Brundin, P. (2014). Progressive nigrostriatal terminal dysfunction and degeneration in the engrailed1 heterozygous mouse model of Parkinson’s disease. Neurobiology of Disease(0).

Saal, K.-A., Koch, J. C., Tatenhorst, L., Szegő, É. M., Ribas, V. T., Michel, U., . . . Lingor, P. (2014). AAV.shRNA-mediated downregulation of ROCK2 attenuates degeneration of dopaminergic neurons in toxin-induced models of Parkinson’s disease in vitro and in vivo. Neurobiology of Disease(0).

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Researchers cited MBF systems in 12 papers during the week of 9/28/14

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Acosta, S. A., Tajiri, N., de la Pena, I., Bastawrous, M., Sanberg, P. R., Kaneko, Y., & Borlongan, C. V. (2014). Alpha-synuclein as a Pathological Link between Chronic Traumatic Brain Injury and Parkinson’s disease. Journal of Cellular Physiology, n/a-n/a. doi: 10.1002/jcp.24830.

Chiu, C.-C., Yeh, T.-H., Lai, S.-C., Wu-Chou, Y.-H., Chen, C.-H., Mochly-Rosen, D., . . . Lu, C.-S. (2014). Neuroprotective effects of aldehyde dehydrogenase 2 activation in rotenone-induced cellular and animal models of parkinsonism. Experimental Neurology(0).

Cyr, M., Parent, M. J., Mechawar, N., Rosa-Neto, P., Soucy, J.-P., Clark, S. D., . . . Bedard, M.-A. (2014). Deficit in sustained attention following selective cholinergic lesion of the pedunculopontine tegmental nucleus in rat, as measured with both post-mortem immunocytochemistry and in vivo PET imaging with [18F]fluoroethoxybenzovesamicol. Behavioural Brain Research(0).

Das, M. M., & Svendsen, C. N. (2014). Astrocytes show reduced support of motor neurons with aging that is accelerated in a rodent model of ALS. Neurobiology of Aging(0).

Kim, A., & Mandyam, C. D. (2014). Methamphetamine affects cell proliferation in the medial prefrontal cortex: A new niche for toxicity. Pharmacology Biochemistry and Behavior(0).

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Researchers cited MBF systems in 16 papers during the week of 9/21/2014

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Beig, M. I., Dampney, B. W., & Carrive, P. (2014). Both ox1r and ox2r orexin receptors contribute to the cardiovascular and locomotor components of the novelty stress response in the rat. Neuropharmacology(0).

Hwang, D. H., Shin, H. Y., Kwon, M. J., Choi, J. Y., Ryu, B.-Y., & Kim, B. G. (2014). Survival of Neural Stem Cell Grafts in the Lesioned Spinal Cord Is Enhanced by a Combination of Treadmill Locomotor Training via Insulin-Like Growth Factor-1 Signaling. The Journal of Neuroscience, 34(38), 12788-12800.

Liu, Y., Zhu, M., Lin, L., Fan, X., Piao, Z., & Jiang, X. (2014). Deficiency of Trim27 protects dopaminergic neurons from apoptosis in the neurotoxin model of Parkinson׳s disease. Brain Research(0).

Lu, W., Karuppagounder, S. S., Springer, D. A., Allen, M. D., Zheng, L., Chao, B., . . . Lenardo, M. (2014). Genetic deficiency of the mitochondrial protein PGAM5 causes a Parkinson’s-like movement disorder. Nature Communications, 5.

Skopin, M. D., Kabadi, S. V., Viechweg, S. S., Mong, J. A., & Faden, A. I. (2014). Chronic decrease in wakefulness and disruption of sleep-wake behavior after experimental traumatic brain injury. Journal of Neurotrauma(ja).

Vuillemenot, B. R., Kennedy, D., Cooper, J. D., Wong, A. M. S., Sri, S., Doeleman, T., . . . O’Neill, C. A. (2014). Nonclinical evaluation of CNS-administered TPP1 enzyme replacement in canine CLN2 neuronal ceroid lipofuscinosis. Molecular Genetics and Metabolism(0).

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Scientists Use Neurolucida to Create 3D Reconstructions of Placental Villous Trees

(a,b) Comparison of the microscopic aspects of a thin (4–6 μm) histological section of a human placenta after staining with hematoxylin/eosin (a) with the microscopic aspects of a whole-mount isolated villous tree after staining with hematoxylin (b). The scale bars in a and b are 250 μm. (a) Various cross- and longitudinal sections of villi can be recognized. The stromal architecture inside the sectioned villi is visible. The cross-sections of branches belong to an unknown number of villous trees. (b) A single villous tree is visible, and branches are not sectioned. The hierarchical positions of nodes (branching points) and the branching topology can be recognized.

(a,b) Comparison of the microscopic aspects of a thin (4–6 μm) histological section of a human placenta after staining with hematoxylin/eosin.

When neuroscientists started studying neurons in 3D, it revolutionized brain science. Now, for the first time, scientists are using this same technology to study the human placenta, and they’ve made some fascinating new discoveries about its structure.

Using Neurolucida to create 3D reconstructions of villous trees – three-dimensional structures in the placenta that facilitate gas and nutrient exchange between the fetus and mother – researchers in Munich, Germany uncovered a wealth of information about their architecture.

For the first time, they analyzed the complexity of villous tree branches and branching, determined the number and location of nodes (branching points), and measured branch angles, discovering a surprising correlation between the branching angle of terminal branchesand the fetoplacental weight ratio (BW/PW) – a calculation commonly used to measure fetal health in prenatal medicine.

“The results show that 3D analysis with Neurolucida reaches beyond the horizons of 2D histology, the current gold standard in placenta morphology/pathology,” said Dr. Hans-Georg Frank, an author of the study. Continue reading “Scientists Use Neurolucida to Create 3D Reconstructions of Placental Villous Trees” »

Researchers cited MBF systems in 15 papers during the week of 9/14/2014

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Bereket, C., Özan, F., Sener, I., Tek, M., Altunkaynak, B. Z., Semirgin, S. U., . . . Özdemir, M. (2014). Propolis Accelerates the Consolidation Phase in Distraction Osteogenesis. Journal of Craniofacial Surgery, 25(5), 1912-1916 1910.1097/SCS.0000000000000946.

Fritschi, S. K., Langer, F., Kaeser, S. A., Maia, L. F., Portelius, E., Pinotsi, D., . . . Jucker, M. (2014). Highly potent soluble amyloid-β seeds in human Alzheimer brain but not cerebrospinal fluid. Brain. doi: 10.1093/brain/awu255.

Gampe, K., Stefani, J., Hammer, K., Brendel, P., Pötzsch, A., Enikolopov, G., . . . Zimmermann, H. (2014). NTPDase2 and Purinergic Signaling Control Progenitor Cell Proliferation in Neurogenic Niches of the Adult Mouse Brain. Stem Cells, n/a-n/a. doi: 10.1002/stem.1846.

Naef, L., Gjerde, E., Long, H., Richard, D., & Walker, C.-D. (2014). Neonatal onset of leptin signaling in dopamine neurons of the ventral tegmental area in the rat. Journal of Neuroendocrinology, n/a-n/a. doi: 10.1111/jne.12221.

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Researchers cited MBF systems in 15 papers during the week of 9/7/2014

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Arruda-Carvalho, M., Restivo, L., Guskjolen, A., Epp, J. R., Elgersma, Y., Josselyn, S. A., & Frankland, P. W. (2014). Conditional Deletion of α-CaMKII Impairs Integration of Adult-Generated Granule Cells into Dentate Gyrus Circuits and Hippocampus-Dependent Learning. The Journal of Neuroscience, 34(36), 11919-11928.

Chou, C.-H., Sinden, J. D., Couraud, P.-O., & Modo, M. (2014). In Vitro Modeling of the Neurovascular Environment by Coculturing Adult Human Brain Endothelial Cells with Human Neural Stem Cells. Plos one, 9(9), e106346. doi: 10.1371/journal.pone.0106346.

Fritschi, S., Cintron, A., Ye, L., Mahler, J., Bühler, A., Baumann, F., . . . Jucker, M. (2014). Aβ seeds resist inactivation by formaldehyde. Acta Neuropathologica, 128(4), 477-484. doi: 10.1007/s00401-014-1339-2.

Godden, K. E., Landry, J. P., Slepneva, N., Migues, P. V., & Pompeiano, M. (2014). Early Expression of Hypocretin/Orexin in the Chick Embryo Brain. Plos one, 9(9), e106977. doi: 10.1371/journal.pone.0106977.

Jeong, S., Lei, B., Wang, H., Dawson, H. N., & James, M. L. (2014). Intravenous immunoglobulin G improves neurobehavioral and histological outcomes after traumatic brain injury in mice. Journal of Neuroimmunology(0).

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Researchers cited MBF systems in 15 papers during the week of 8/31/2014

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Apland, J. P., Aroniadou-Anderjaska, V., Figueiredo, T. H., Rossetti, F., Miller, S. L., & Braga, M. F. M. (2014). The Limitations of Diazepam as a Treatment for Nerve Agent-Induced Seizures and Neuropathology in Rats; Comparison with UBP302. Journal of Pharmacology and Experimental Therapeutics. doi: 10.1124/jpet.114.217299.

Aydin, C., Oztan, O., & Isgor, C. (2014). Hippocampal Y2 receptor-mediated mossy fiber plasticity is implicated in nicotine abstinence-related social anxiety-like behavior in an outbred rat model of the novelty-seeking phenotype. Pharmacology Biochemistry and Behavior, 125(0), 48-54.

Bae, E.-J., Yang, N.-Y., Song, M., Lee, C. S., Lee, J. S., Jung, B. C., . . . Lee, S.-J. (2014). Glucocerebrosidase depletion enhances cell-to-cell transmission of α-synuclein. [Article]. Nat Commun, 5. doi: 10.1038/ncomms5755.

Baleriola, J., Walker, Chandler A., Jean, Ying Y., Crary, John F., Troy, Carol M., Nagy, Peter L., & Hengst, U. (2014). Axonally Synthesized ATF4 Transmits a Neurodegenerative Signal across Brain Regions. Cell, 158(5), 1159-1172.

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