Researchers cited MBF systems in 29 papers between 6/30/2017 to 7/14/2017

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Abdurakhmanova, S., Chary, K., Kettunen, M., Sierra, A., & Panula, P. (2017). Behavioral and stereological characterization of Hdc KO mice: relation to Tourette syndrome. Journal of Comparative Neurology, n/a-n/a. doi: 10.1002/cne.24279.

Arathoon, L. R., Gleave, J. A., Trinh, D., Lizal, K. E., Giguère, N., Barber, J. H. M., . . . Nash, J. E. (2017). Sirtuin 3 rescues neurons through the stabilisation of mitochondrial biogenetics in the virally-expressing mutant α-synuclein rat model of parkinsonism. Neurobiology of Disease. doi:

Chen, L., Wang, X., Lin, Z.-X., Dai, J.-G., Huang, Y.-F., & Zhao, Y.-N. (2017). Preventive Effects of Ginseng Total Saponins on Chronic Corticosterone-Induced Impairment in Astrocyte Structural Plasticity and Hippocampal Atrophy. Phytotherapy Research, n/a-n/a. doi: 10.1002/ptr.5859.

Collette, J. C., Choubey, L., & Smith, K. M. (2017). ­Glial and stem cell expression of murine Fibroblast Growth Factor Receptor 1 in the embryonic and perinatal nervous system. PeerJ, 5, e3519. doi: 10.7717/peerj.3519.

Filice, F., Celio, M. R., Babalian, A., Blum, W., & Szabolcsi, V. (2017). Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice. Journal of Comparative Neurology, n/a-n/a. doi: 10.1002/cne.24276.

Finkelstein, D. I., Billings, J. L., Adlard, P. A., Ayton, S., Sedjahtera, A., Masters, C. L., . . . Cherny, R. A. (2017). The novel compound PBT434 prevents iron mediated neurodegeneration and alpha-synuclein toxicity in multiple models of Parkinson’s disease. Acta Neuropathologica Communications, 5(1), 53. doi: 10.1186/s40478-017-0456-2.

Han, S.-W., Kim, Y.-C., & Narayanan, N. S. (2017). Projection targets of medial frontal D1DR-expressing neurons. Neuroscience Letters. doi:

Lee, J. Q., Sutherland, R. J., & McDonald, R. J. (2017). Hippocampal damage causes retrograde but not anterograde memory loss for context fear discrimination in rats. Hippocampus, n/a-n/a. doi: 10.1002/hipo.22759.

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Researchers cited MBF systems in 8 papers during the week of 06/19/2017

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Kaur, C., Pal, I., Saini, S., Jacob, T. G., Nag, T. C., Thakar, A., . . . Roy, T. S. (2017). Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. Journal of Chemical Neuroanatomy. doi:

Mani, B. K., Osborne-Lawrence, S., Mequinion, M., Lawrence, S., Gautron, L., Andrews, Z. B., & Zigman, J. M. (2017). The role of ghrelin-responsive mediobasal hypothalamic neurons in mediating feeding responses to fasting. Molecular Metabolism. doi: 10.1016/j.molmet.2017.06.011.

Martinez, E. M., Young, A. L., Patankar, Y. R., Berwin, B. L., Wang, L., von Herrmann, K. M., . . . Havrda, M. C. (2017). Nlrp3 is required for inflammatory changes and nigral cell loss resulting from chronic intragastric rotenone exposure in mice. Toxicological Sciences.…

Po, K. K.-t., Leung, J. W.-h., Chan, J. N.-m., Fung, T. K.-h., Sánchez-Vidaña, D. I., Sin, E. L.-l., . . . Siu, A. M.-h. (2017). Protective effect of Lycium Barbarum polysaccharides on dextromethorphan-induced mood impairment and neurogenesis suppression. Brain Research Bulletin, 134, 10-17. doi:  Continue reading “Researchers cited MBF systems in 8 papers during the week of 06/19/2017” »

Researchers cited MBF systems in 25 papers between 6/10/2017 and 6/16/2017

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Arawaka, S., Sato, H., Sasaki, A., Koyama, S., & Kato, T. (2017). Mechanisms underlying extensive Ser129-phosphorylation in α-synuclein aggregates. Acta Neuropathologica Communications, 5(1), 48.

Birkl, C., Carassiti, D., Hussain, F., Langkammer, C., Enzinger, C., Fazekas, F., . . . Ropele, S. (2017). Assessment of ferritin content in multiple sclerosis brains using temperature-induced R*2 changes. Magnetic Resonance in Medicine, n/a-n/a. doi: 10.1002/mrm.26780.

Boudewyn, L. C., Sikora, J., Kuchar, L., Ledvinova, J., Grishchuk, Y., Wang, S. L., . . . Walkley, S. U. (2017). N-butyl-deoxynojirimycin delays motor deficits, cerebellar microgliosis, and Purkinje cell loss in a mouse model of mucolipidosis type IV. Neurobiology of Disease. doi:

Chaves, P. P., Valdoria, C. M., Amorim, M. C. P., & Vasconcelos, R. O. (2017). Ontogenetic development of the inner ear saccule and utricle in the Lusitanian toadfish: Potential implications for auditory sensitivity. Hearing Research. doi:

Ebenezer, G. J., Liu, Y., Judge, D. P., Cunningham, K., Truelove, S., Carter, N. D., . . . Polydefkis, M. (2017). Cutaneous nerve biomarkers in transthyretin familial amyloid polyneuropathy. Annals of Neurology, n/a-n/a. doi: 10.1002/ana.24972.

Fischer, D. L., Kemp, C. J., Cole-Strauss, A., Polinski, N. K., Paumier, K. L., Lipton, J. W., . . . Sortwell, C. E. (2017). Subthalamic Nucleus Deep Brain Stimulation Employs TrkB Signaling for Neuroprotection and Functional Restoration. The Journal of Neuroscience.

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Researchers cited MBF systems in 25 papers between 5/27/2017 and 6/9/2017

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Bastidas, J., Athauda, G., De La Cruz, G., Chan, W.-M., Golshani, R., Berrocal, Y., . . . Pearse, D. D. (2017). Human schwann cells exhibit long-term cell survival, are not tumorigenic and promote repair when transplanted into the contused spinal cord. Glia, n/a-n/a. doi: 10.1002/glia.23161.

Claflin, D. I., Schmidt, K. D., Vallandingham, Z. D., Kraszpulski, M., & Hennessy, M. B. (2017). Influence of postnatal glucocorticoids on hippocampal-dependent learning varies with elevation patterns and administration methods. Neurobiology of Learning and Memory. doi:

Dioli, C., Patricio, P., Trindade, R., Pinto, L. G., Silva, J. M., Morais, M., . . . Sotiropoulos, I. (2017). Tau-dependent suppression of adult neurogenesis in the stressed hippocampus.  Molecular Psychiatry. doi: 10.1038/mp.2017.103.

Giesert, F., Glasl, L., Zimprich, A., Ernst, L., Piccoli, G., Stautner, C., . . . Wurst, W. (2017). The pathogenic LRRK2 R1441C mutation induces specific deficits modeling the prodromal phase of Parkinson’s disease in the mouse. Neurobiology of Disease. doi:

Gumusoglu, S. B., Fine, R. S., Murray, S. J., Bittle, J. L., & Stevens, H. E. (2017). The role of IL-6 in neurodevelopment after prenatal stress. Brain, Behavior, and Immunity. doi:

Issa, J. P. M., Trawitzki, B. F., Ervolino, E., Macedo, A. P., & Lilge, L. (2017). Low-intensity laser therapy efficacy evaluation in FVB mice subjected to acute and chronic arthritis. [journal article]. Lasers in Medical Science, 1-9. doi: 10.1007/s10103-017-2235-5.

Liu, H., Rose, M. E., Ma, X., Culver, S., Dixon, C. E., & Graham, S. H. (2017). In vivo transduction of neurons with TAT-UCH-L1 protects brain against controlled cortical impact injury. Plos one, 12(5), e0178049. doi: 10.1371/journal.pone.0178049.

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Researchers cited MBF systems in 25 papers between 5/16/2017 and 5/26/2017

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Baxter, V. K., Glowinski, R., Braxton, A. M., Potter, M. C., Slusher, B. S., & Griffin, D. E. (2017). Glutamine antagonist-mediated immune suppression decreases pathology but delays virus clearance in mice during nonfatal alphavirus encephalomyelitis. Virology, 508, 134-149. doi:

Brzozowska, N. I., Smith, K. L., Zhou, C., Waters, P. M., Cavalcante, L. M., Abelev, S. V., . . . Arnold, J. C. (2017). Genetic deletion of P-glycoprotein alters stress responsivity and increases depression-like behavior, social withdrawal and microglial activation in the hippocampus of female mice. Brain, Behavior, and Immunity. doi:

Chareyron, L. J., Banta Lavenex, P., Amaral, D. G., & Lavenex, P. (2017). Functional organization of the medial temporal lobe memory system following neonatal hippocampal lesion in rhesus monkeys. Brain Structure and Function, 1-16. doi: 10.1007/s00429-017-1441-z.

Kwan, T., Floyd, C. L., Patel, J., Mohaimany-Aponte, A., & King, P. H. (2017). Astrocytic expression of the RNA regulator HuR accentuates spinal cord injury in the acute phase. Neuroscience Letters, 651, 140-145. doi:

Newville, J., Valenzuela, C. F., Li, L., Jantzie, L. L., & Cunningham, L. A. (2017). Acute oligodendrocyte loss with persistent white matter injury in a third trimester equivalent mouse model of fetal alcohol spectrum disorder. Glia, n/a-n/a. doi: 10.1002/glia.23164.

Onger, M. E., Kaplan, S., Geuna, S., Türkmen, A. P., Muratori, L., Altun, G., & Altunkaynak, B. Z. (2017). Possible effects of some agents on the injured nerve in obese rats: A stereological and electron microscopic study. Journal of Cranio-Maxillofacial Surgery. doi:

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Researchers cited MBF systems in 17 papers during the week of 05/08/2017

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Day, N. L., Carle, M. S., & Floyd, C. L. (2017). Post-injury administration of a combination of memantine and 17β-estradiol is protective in a rat model of traumatic brain injury. Neurochemistry International. doi:

El-Agnaf, O., Overk, C., Rockenstein, E., Mante, M., Florio, J., Adame, A., . . . Rissman, R. A. (2017). Differential effects of immunotherapy with antibodies targeting α-synuclein oligomers and fibrils in a transgenic model of synucleinopathy. Neurobiology of Disease, 104, 85-96. doi:

Matsuda, W., Ehara, A., Nakadate, K., Yoshimoto, K., & Ueda, S. (2017). Effects of environmental enrichment on the activity of the amygdala in micrencephalic rats exposed to a novel open field. Congenital Anomalies, n/a-n/a. doi: 10.1111/cga.12228.

Musacchio, T., Rebenstorff, M., Fluri, F., Brotchie, J. M., Volkmann, J., Koprich, J. B., & Ip, C. W. (2017). STN-DBS is neuroprotective in the A53T α-synuclein Parkinson’s disease rat model. Annals of Neurology, n/a-n/a. doi: 10.1002/ana.24947.

Soares, J. I., Valente, M. C., Andrade, P. A., Maia, G. H., & Lukoyanov, N. V. (2017). Reorganization of the septohippocampal cholinergic fiber system in experimental epilepsy. Journal of Comparative Neurology, n/a-n/a. doi: 10.1002/cne.24235.

Yoshii, Y., Inoue, T., Uemura, Y., Iwasaki, Y., Yada, T., Nakabeppu, Y., & Noda, M. (2017). Complexity of Stomach–Brain Interaction Induced by Molecular Hydrogen in Parkinson’s Disease Model Mice. Neurochemical Research, 1-8. doi: 10.1007/s11064-017-2281-1.  Continue reading “Researchers cited MBF systems in 17 papers during the week of 05/08/2017” »

Researchers cited MBF systems in 42 papers between 4/7/2017 and 4/21/2017

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Ay, M., Luo, J., Langley, M., Jin, H., Anantharam, V., Kanthasamy, A., & Kanthasamy, A. G. (2017). Molecular Mechanisms Underlying Protective Effects of Quercetin Against Mitochondrial Dysfunction and Progressive Dopaminergic Neurodegeneration in Cell Culture and MitoPark Transgenic Mouse Models of Parkinson’s Disease. Journal of Neurochemistry, n/a-n/a. doi: 10.1111/jnc.14033.

Carryl, H., Van Rompay, K., De Paris, K., & Burke, M. (2017). Hippocampal Neuronal Loss in Infant Macaques Orally Infected with Virulent Simian Immunodeficiency Virus (SIV). Brain Sciences, 7(4), 40.

Galinato, M. H., Lockner, J. W., Fannon-Pavlich, M. J., Sobieraj, J. C., Staples, M. C., Somkuwar, S. S., . . . Mandyam, C. D. (2017). A synthetic small-molecule Isoxazole-9 protects against methamphetamine relapse. Molecular Psychiatry. doi: 10.1038/mp.2017.46.

Gilles, Y. D., & Polston, E. K. (2017). Effects of social deprivation on social and depressive-like behaviors and the numbers of oxytocin expressing neurons in rats. Behavioural Brain Research SreeTestContent1, 328, 28-38. doi:

Hamilton, G. F., Hernandez, I. J., Krebs, C. P., Bucko, P. J., & Rhodes, J. S. (2017). Neonatal alcohol exposure reduces number of parvalbumin-positive interneurons in the medial prefrontal cortex and impairs passive avoidance acquisition in mice deficits not rescued from exercise. Neuroscience. doi:

Jinhua, H., Zhao, M., Shuangcheng, H., Yuanran, Z., Rui, N., Wei, L., . . . Jian, Y. (2017). Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3β/β-catenin integrated signaling pathway. Biochemical Pharmacology. doi:

Kafetzopoulos, V., Kokras, N., Sotiropoulos, I., Oliveira, J. F., Leite-Almeida, H., Vasalou, A., . . . Dalla, C. (2017). The nucleus reuniens: a key node in the neurocircuitry of stress and depression. Molecular Psychiatry. doi: 10.1038/mp.2017.55.

Lear, C. A., Davidson, J. O., Mackay, G. R., Drury, P. P., Galinsky, R., Quaedackers, J. S., . . . Bennet, L. (2017). Antenatal dexamethasone before asphyxia promotes cystic neural injury in preterm fetal sheep by inducing hyperglycemia. Journal of Cerebral Blood Flow and Metabolism, 0271678X17703124. doi: 10.1177/0271678×17703124.

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MBF Bioscience Unveils Redesigned Interface for Neurolucida and Stereo Investigator version 2017

Neuroscientists can now analyze the size and complexity of neurons and collect unbiased stereology data with greater speed and efficiency

We are happy to announce the release of Neurolucida and Stereo Investigator version 2017. This version features a completely revamped user interface that’s intuitive and easy to navigate.

“Neurolucida and Stereo Investigator version 2017 are completely redesigned to improve the user experience and increase productivity,” said Jack Glaser, President of MBF Bioscience. “The new interface makes collecting accurate data much quicker and easier.”

Version 2017 has many new performance features in addition to the new user interface. It handles large image data more efficiently, gives users the ability to dynamically edit digital reconstructions in an interactive 3D environment, and much more. Neurolucida users will also see many improvements to Neurolucida Explorer – the companion software to Neurolucida that performs all the quantitative analyses generated from neurons that are digitally reconstructed with Neurolucida.

Highlights of Stereo Investigator and Neurolucida version 2017 include:

  • Easier Navigation – The ribbon bar design is task-oriented to make it easy to find what you need. A dynamic search bar and a quick access toolbar also aid productivity.
  • Improved Organization – Frequently used tools are prominent and easy to access. Tools are grouped by their function, and advanced settings are easily accessible.
  • Cleaner and simpler – The new user interface is modern and intuitive, making it easier to learn and to train new lab members.
  • Support for the latest technological advancements in microscopic imaging devices and computer hardware.

See version 2017 in action

MBF Bioscience will exhibit and demo Neurolucida and Stereo Investigator version 2017 at the annual Experimental Biology meeting April 23 – 25 in Chicago, Illinois.

Get a quick overview of the new user interface in these 2-minute videos:

Register for the upcoming webinar “Using the Optical Fractionator Probe to Estimate Number of Cells”

Researchers cited MBF systems in 27 papers between 3/20/2017 and 3/30/2017

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Abe, C., Inoue, T., Inglis, M. A., Viar, K. E., Huang, L., Ye, H., . . . Guyenet, P. G. (2017). C1 neurons mediate a stress-induced anti-inflammatory reflex in mice. Nature Neuroscience, advance online publication. doi: 10.1038/nn.4526

Caldwell, A. S. L., Edwards, M. C., Desai, R., Jimenez, M., Gilchrist, R. B., Handelsman, D. J., & Walters, K. A. (2017). Neuroendocrine androgen action is a key extraovarian mediator in the development of polycystic ovary syndrome. Proceedings of the National Academy of Sciences. doi: 10.1073/pnas.1616467114.

Castro-Hernández, J., Adlard, P. A., & Finkelstein, D. I. (2017). Pramipexole restores depressed transmission in the ventral hippocampus following MPTP-lesion. Scientific Reports, 7, 44426. doi: 10.1038/srep44426.

Dawes, W. J., Zhang, X., Fancy, S. P., Rowitch, D., & Marino, S. (2017). Moderate-Grade Germinal Matrix Haemorrhage Activates Cell Division in the Neonatal Mouse Subventricular Zone. Developmental Neuroscience.

Drobyshevsky, A., & Quinlan, K. A. (2017). Spinal cord injury in hypertonic newborns after antenatal hypoxia-ischemia in a rabbit model of cerebral palsy. Experimental Neurology, 293, 13-26. doi:

El Massri, N., Lemgruber, A. P., Rowe, I. J., Moro, C., Torres, N., Reinhart, F., . . . Mitrofanis, J. (2017). Photobiomodulation-induced changes in a monkey model of Parkinson’s disease: changes in tyrosine hydroxylase cells and GDNF expression in the striatum. Experimental Brain Research, 1-14. doi: 10.1007/s00221-017-4937-0.

Hühner, L., Rilka, J., Gilsbach, R., Zhou, X., Machado, V., & Spittau, B. (2017). Interleukin-4 Protects Dopaminergic Neurons In vitro but Is Dispensable for MPTP-Induced Neurodegeneration In vivo. Frontiers in Molecular Neuroscience, 10(62). doi: 10.3389/fnmol.2017.00062.

Meng, L., Huang, T., Sun, C., Hill, D. L., & Krimm, R. (2017). BDNF is required for taste axon regeneration following unilateral chorda tympani nerve section. Experimental Neurology, 293, 27-42. doi:

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A complete guide to imaging and analyzing spines and neurons with Neurolucida 360

Following a well-designed protocol is essential to achieving accurate and consistent results in scientific research. Now, scientists using Neurolucida 360 for dendritic spine and neuron analysis can follow a published set of guidelines to ensure optimal confocal data series for proper dendritic spine quantification and neuron reconstruction. The paper, written by MBF Bioscience scientists and researchers from the Icahn School of Medicine at Mount Sinai in New York, was published in Current Protocols in Neuroscience.

The four protocols describe best practices for imaging and analyzing dendritic spines and entire neurons. Clearly laid out procedures specify necessary materials, image acquisition techniques, and analysis procedures with Neurolucida 360.

Imaging technique is crucial to obtaining unbiased, reproducible results. Clear, crisp images captured with an appropriate z-interval will make analysis with Neurolucida 360 easier and more accurate. Throughout the paper, the authors emphasize the importance of image scaling parameters and unbiased sampling for achieving repeatable results. They also discuss the benefits of correcting optical distortion, especially in the Z-plane, with deconvolution to acquire clear images – a process critical to getting the most accurate representation of dendrites and spines.

Dendritic spine analysis is traditionally performed through tedious, time-consuming manual techniques. According to the paper, this has spawned a growing interest in a more efficient solution for spine quantification and morphological analysis like the one Neurolucida 360 provides. A software platform for automatic neuron reconstruction and spine detection in a 3D environment, Neurolucida 360 offers a variety of benefits, including:


  • Fast and accurate spine detection and neuron reconstruction
  • Accurate spine classification and length quantification using a five-point segment that more accurately models the spine backbone.
  • 3 user-guided and automatic algorithms to accurately model neurons visualized with multiple methodologies and imaging techniques.
  • A large number of metrics, including volume, length, and surface area.


“We believe that the new quantitative software package, Neurolucida 360, provides the neuroscience research community with the ability to perform higher throughput automated 3D quantitative light microscopy spine analysis under standardized conditions to accelerate the characterization of dendritic spines with greater objectivity and reliability,” (Dickstein, et al. 2016)

The full paper can be found here.

An infographic quickly outlines Protocol 1: Imaging of fluorescently labeled dendritic segments. Use this as a quick reference tool in your lab (right-click on it to save as an image):

Dickstein, D.L., Dickstein, D.R., Janssen, W.G.M., Hof, P.R., Glaser, J.R., Rodriguez, A., O’Connor, N., Angstman, P., and Tappan, S.J. 2016. Automatic dendritic spine quantification from confocal data with Neurolucida 360. Curr. Protoc. Neurosci. 77:1.27.1-1.27.21. doi: 10.1002/cpns.16