Mapping the Neurons of the Rat Heart in 3D
An interdisciplinary team of researchers, including MBF Bioscience’s Dr. Susan Tappan and Maci Heal, have created a fully reconstructed, virtual 3D
TissueMaker®
Automatically align serial sections and visualize an entire 3D organProduct Overview
TissueMaker automatically creates full-resolution 3D reconstructions of entire organs or other structures from serial sections or whole slide images. Simply load high resolution images of serial sections acquired from a slide scanner or research microscope, then let the software do the work of automatically aligning sections and reconstructing the specimen in 3D.
With just a glance and full anatomical context, you can identify cells or structures that express a particular gene or visualize morphologies that span multiple individual fields of view. Use TissueMaker to assist you with cell mapping, cytoarchitectonics and other characteristics of organs or structures to create a comprehensive anatomical reference.
Key Benefits
- Create easy-to-visualize 3D volumes from series of sections
- Control the reconstruction process as much or as little as desired using an intuitive workflow
- Start with almost any whole slide image format; most are supported
- Visualize reconstructions as a 3D volume, sliced image, or multiple projections
- Save to an open and FAIR file format for easy collaboration
TissueMaker generates high-resolution 3D volume reconstructions from serial sections imaged using whole slide scanners and research microscopes. Load the images into TissueMaker and then direct your attention to other projects while TissueMaker automatically detects the individual sections on each slide, and then aligns the sections to create the full 3D organ reconstruction. Image features found in multiple serial sections are automatically aligned using innovative computational algorithms.
It's fast. And it's smart. If you mounted a section upside down, TissueMaker automatically corrects it during the alignment process. If additional adjustments need to be made to the automatic alignment, you can easily edit the 3D reconstruction.
| Minimum Hardware Requirements |
|---|
| 64-bit Windows 10 operating system |
| 8-core processor |
| Solid state drive(s) |
| NVIDIA 1060 graphics card (1060=6GB) |
Compatible image file formats: View PDF
Mapping the Neurons of the Rat Heart in 3D
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A GPS for the brain and so much more
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Download TissueMaker product sheet here.
TissueMaker: Key Features
Real-time interactive 3D visualization
Use the 3D anatomical reference created with TissueMaker to make measurements within the entire organ, something that is not possible using the serial sections directly. Analyze the size and shape of structures within the 3D reconstruction using Neurolucida 360. Or assess cell distribution within a region using stereology with Stereo Investigator.
In this video, spinal cord sections were aligned using TissueMaker to visualize the rostro-caudal distribution of sensory inputs from the LUT. CTB-labelled LUT afferents in the sacral dorsal commissural nucleus and sacral preganglionic nucleus were detected and further analyzed in Neurolucida 360 and Neurolucida Explorer.
Read the paper: https://doi.org/10.1523/ENEURO.0364-21.2021
Video Courtesy: Drs. Fuller-Jackson, Osborne, and Keast at the University of Melbourne
TissueMaker: Key Features
View, store and share your whole-slide images and 3D reconstructions
Reconstructions created with TissueMaker can be stored, viewed, and shared with Biolucida – our platform for organizing and sharing big image data. Never run out of storage space, or spend hours searching for an image again. And instead of publishing a 2D image of your 3D reconstruction, you can share the actual 3D reconstruction and allow others to dynamically visualize it using our free Biolucida 3D image viewer.
TissueMaker: Key Features
Real-time interactive 3D visualization
TissueMaker includes a highly versatile 3D visualization environment suitable to most microscopy images, with state-of-the-art functionalities to support your analysis and publication needs.
- Visualize large, complex 3D images (including multichannel images) and reconstructions
- 2D and 3D image data from two-photon, confocal, brightfield or light sheet microscopy
- 3D volume, image slicing, and simultaneous views
- Multiple projections: XY, YZ, XZ, alpha, minimum, and maximum
- Transparency, rendering (wireframe, centerline, surface, texture) and other display options for images and traced structures that can be adjusted on the fly
- Loads complex surfaces in seconds
- Export options include 3D graphics suitable for third-party applications
TissueMaker: Key Features
Generate DATA that's standardized, reliable and FAIR
We support open science through data sharing, accessibility, integrity, and reproducibility. The published MBF Bioscience digital reconstruction data file format, the Neuromorphological File Specification, was recently endorsed as a standard by the International Neuroinformatics Coordinating Facility (INCF).
The data elements in this file format were implemented specifically to ensure that files produced in TissueMaker are findable, accessible, interoperable, and reusable (FAIR). TissueMaker embraces open science data standards and provides microscopy image and experimental data provenance to enhance the ease of repurposing data generated with TissueMaker. Encoded in the well-recognized and readable XML format, the modeling elements specify anatomical information in a calibrated 3D coordinate system with appropriate units. To learn more about the key elements of the file format and its relevant structural advantages, read our publication.
Who Is Using TissueMaker?
TissueMaker is used across the globe by the most prestigious laboratories.
News About TissueMaker
MBF Bioscience receives NIH funding to support innovative research program on the peripheral nervous system
FOR IMMEDIATE RELEASE MBF Bioscience Williston, VT – January 9, 2019 – MBF Bioscience is pleased to announce our participation in the Stimulating
Cited in Peer Reviewed Scientific Publications
The utility of MBF’s products is underscored by the number of references it receives in the world’s leading scientific publications. See examples below:
Wang, Z., D. Zheng, et al.
Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic BrainView Publication
da Silva, S. P., C. C. M. de Castro, et al.
Neuropathological and sociodemographic factors associated with the cortical amyloid load in aging and Alzheimer’s diseaseView Publication
Jovanoski, K. D., L. Duquenoy, et al.
Dopaminergic systems create reward seeking despite adverse consequencesView Publication
Gergues, M. M., K. J. Han, et al.
“Circuit and molecular architecture of a ventral hippocampal network.”View Publication
Lindberg, P. T., J. W. Mitchell, et al.
“Pituitary Adenylate Cyclase-Activating Peptide (PACAP)-Glutamate Co-transmission Drives Circadian Phase-Advancing Responses to Intrinsically Photosensitive Retinal Ganglion Cell Projections by Suprachiasmatic Nucleus.”View Publication
Zhang, L., V. S. Hernandez, et al.
"Behavioral role of PACAP signaling reflects its selective distribution in glutamatergic and GABAergic neuronal subpopulations." eLife 10: e61718.View Publication
Frequently Asked Questions (FAQ)
What image files can I use with TissueMaker?
TissueMaker works with images acquired from most slide scanners and research microscope imaging systems.
What imaging modality is supported by TissueMaker?
Any single-plane group of images will work, whether they come from a slide scanner, brightfield or fluorescence wide-field microscope or confocal. TissueMaker can convert your multiplane images encompassing your entire section depth to a max projection or extended depth of focus single-plane image (i.e. using our free software MicroFile+) for ulterior analysis with TissueMaker.
Can I use TissueMaker software with any type of organ?
Absolutely. TissueMaker uses the shape and image information to do the alignment irrespectively of the tissue, species, sectioning orientation, markers or labels you are using.
Can TissueMaker resolve inverted sections?
Yes. TissueMaker uses the shape and image information across multiple sections to detect laterality and flip the sections to the right orientation.
Testimonials
"I rarely have encountered a company so committed to support and troubleshooting as MBF."
"MBF Bioscience is extremely responsive to the needs of scientists and is genuinely interested in helping all of us in science do the best job we can."
"I am so happy to be a customer of your company. I always get great help related with your product or not. With the experienced members, you are the best team I've ever met. All of your staff are very kind and helpful. Thank you for your great help and support all the time."
"We’ve been very happy for many years with MBF products and the course of upgrades and improvements. Your service department is outstanding. I have gotten great help from the staff with the software and hardware."
"Our experience with the MBF equipment and especially the MBF people has been outstanding. I cannot speak any higher about their professionalism and attention for our needs."
"MBF provides excellent technical support and helps you to find the best technical tools for your research challenges on morphometry."
Robust Professional Support
Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron reconstruction, and image processing. We’ve also developed a host of additional support services, including:
- Forums
We have over 25 active forums where open discussions take place.
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We’ve conducted over 750 remote software installations.
>> Learn More - Webinars
We’ve created over 55 webinars that demonstrate our products & their uses.
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Request an Expert Demonstration
We offer both a free demonstration and a free trial copy of TissueMaker. During your demonstration you’ll also have the opportunity to talk to us about your hardware, software, or experimental design questions with our team of Ph.D. neuroscientists and experts in microscopy, neuron tracing, and image processing.
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