Reconstruct and perform complex analyses of microvasculature
Researchers and clinicians are increasingly taking interest in the contribution of blood circulation within neural tissues to neurological disorders and disease states. Vessel microspasms, occlusions, constrictions, and neovascularization have all been shown to contribute to a number of nervous system disorders within the brain, retina, and peripheral tissues. As the body of knowledge in microvessel anatomy and physiology continues to expand, the scientific community has sought to develop accurate and reliable tools for reconstructing and analyzing properties of microvessels in healthy and abnormal tissues. Microvessel reconstruction and 3D rendering however is computationally intensive due to the density of microvessels, necessitating powerful computer graphics systems and complicated algorithms beyond the expertise of most life science research laboratories.
Neuroscientists at MBF Bioscience have developed user-friendly software programs for performing detailed quantitative analysis of microvessel anatomy. Vesselucida and Vesselucida 360 provide automated and manual vessel tracing tools in an interactive 3D modelling environment for efficiently navigating through tissue layers. Automatic vessel reconstruction can provide validation of manual vessel tracings, adding credibility to measurements and the statistical analyses submitted for publication. As with all MBF Bioscience products the vessel reconstruction programs are continuously being updated with new features based on the feedback obtained from scientists actively using the software, saving reearchers the cost and time of hiring a software developer for individual projects. We developed Vesselucida and Vesselucida 360 as tools to allow scientists to quickly and reliably obtain quantifiable measurements of vasculature with minimal training and expense. The Neuroscientists at MBF Bioscience use Vesselucida in their own research projects and are available to assist academic and institutional researchers with their investigations at any step in the research process from image acquisition to publication.
Microvessel quantitative analysis is performed to study
- Cerebral angiogenesis following ischemic stroke or trauma
- Cognitive impairment due to local infarction and ischemia, such as in Alzheimers disease and related dementias
- Recovery from microvascular injury
- Retinal neovascularization in diseases such as diabetic retinopathy and age-related macular degeneration
- Irregularities in capillary loops from angiogenesis in Reynaud Syndrome
- Vessel length, diameter, density, volume, and branching
- Distribution of microvascular damage
- Brain infarcts
- Dichotomic branching
- Tortuous loops
- Rarefication of vessels
- Small vessel cerebrovascular disease