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Please note: BrainMaker can register and align single plane image data from a variety of image file formats. Common file formats include: SVS, NDPI, JPEG2000 (JP2), ND2, LSM, OIB, IDS/ICS, and nearly all CZI. If you have a TIFF file format that exceeds 4GB in size, please convert the image to a BTF format for the evaluation.