"Neurolucida 360 is a remarkable system that has provided us with tools to study dendritic architecture in cultured neurons, rodent models, and humans, with outstanding precision and detail."
Jeremy Herskowitz, Ph.D.
University of Alabama at Birmingham School of Medicine
"Neurolucida 360 is very useful for doing quantitative analysis of neuronal morphology. Multiple parameters extracted by using this quantitative analysis program represent the intrinsic features of neurons. For example, axonal segment lengths, maximum branch angles and bouton density usually stay consistent for a neuron type although the general axonal and dendritic clusters may vary significantly according to the locations of the neuron type. When combined with multiple-neuron patch clamp recording, the quantitative morphological analysis has served as an essential step to enable us to successfully carry out the following research. We have been able to reveal the organizing principles of inhibitory networks (Gupta et al., 2000); define and systematically study a new subclass of basket cell – nest basket cell (Wang et al., 2002); systematically study Martinotti cells in anatomy, electrophysiology and synaptology (Wang et al., 2004); discover glutamatergic differential innervation of different target neurons by single PCs (Markram et al., 1998) and anatomical principles for differential glutamatergic innervation based on a large number of reconstructed connections (Wang et al., 1999). In addition, we have been able to reveal cellular, synaptic and network features of the layer 5 pyramidal microcircuitry (Wang et al., 2006). Furthermore, a unique reconstruction data base of over ten thousand neurons and hundreds of synaptic connections has been developed, which will be essential to put the neuronal microcircuitry together with the biologically realistic computer simulations in the Blue Brain Project."
Yun Wang, Ph.D.
Allen Institute for Brain Science
"Neurolucida 360 is clearly the best in the field - reliable, accurate, and very importantly, easy and intuitive to use. I would not try any other system."
Jeffrey Kordower, Ph.D.
Rush Presbyterian Medical Center
"Neurolucida 360 is the future of quantitative neuromorphology. I am particularly excited about Neurolucida 360's interactive function because it will allow for much more efficient tracing of Golgi stained neurons, which are notoriously difficult to quantify. Cajal would be proud!"
Bob Jacobs, Ph.D.
"I am amazed at the speed and accuracy of Neurolucida 360. The automatic tracing program is intuitive and easy to use."
Gwen Wendelschafer-Crabb, Ph.D.
University of Minnesota