A New and Improved Lucivid


For the first time ever a color microdisplay is available for your microscope.  The new Lucivid LED model let’s you see your head-down display projected over your specimen in color or monochrome.

The Lucivid is an innovative microdisplay that superimposes your computer monitor onto the microscope’s field of view.  Use the Lucivid to perform stereology with Stereo Investigator as well as accurately trace neurons with Neurolucida while you look through your microscope’s eyepieces with your computer’s monitor projected into your microscope.  You can also use the Lucivid for retinal stimulation to excite your retinal specimens by projecting computer generated graphics and patterns onto your specimen.

Smaller, lighter, and more durable than the previous model; the new Lucivid LED model is now available.  Find out more about the new Lucivid LED model on our website.


Register for Our Upcoming Stereology Webinar

The Optical Fractionator probe in Stereo Investigator is an extremely effective tool for stereological cell quantification. It is our goal to provide our customers with the most efficient methods and tools for their research needs.

Join our staff scientists Drs. Jose Maldonado and Daniel Peruzzi on Wednesday, February 22nd at 12:00pm EST for a webinar on using on using unbiased stereology to accurately determine the number of cells in a region of interest.  Our knowledgeable staff scientists, who use the Optical Fractionator probe extensively in their own research, will explain how the Optical Fractionator probe can be applied to your research.


Please click here to register and read the full abstract.

Our Confocal Stereology Webinar Recording is Available to View Online

Confocal microscopy offers clear access to the tiniest biological elements. Stereology provides an exceptional way to quantify them. Combine the two and you’ve got an unparalleled tool for conducting research.

Our latest webinar “Confocal Stereology: A Technical Innovation from MBF Bioscience and Carl Zeiss Microscopy” provides an in-depth look at confocal microscopy, stereology, and how the two can work together. MBF Bioscience Staff Scientists Dr. Susan Hendricks and Dr. Jose Maldonado demonstrate how a Zeiss LSM confocal microscope powered by Zeiss Zen and Stereo Investigator® is a powerful tool for quantifying spines, synapses, co-labeled cells, or fibers in a variety of biological tissues.

Watch the webinar for step-by-step instructions on how to acquire and analyze confocal SRS Image Stacks, see how much easier it to see bright tightly packed small objects in a confocal image, and find out why Stereo Investigator along with Zeiss Zen is the most tightly integrated and easy to use solution for confocal systematic random sampling stereology.

To find out about upcoming MBF Bioscience instructional webinars, “Like” us on Facebook and follow us on Twitter.


Upcoming Webinar: Confocal Stereology – A Technical Innovation from MBF Bioscience and Carl Zeiss

On Thursday, October 6, Dr. Jose Maldonado and Dr. Susan Hendricks present a live webinar on confocal stereology with Stereo Investigator and the ZEISS LSM confocal microscope.

Stereological quantification of cells, synapses, or other very small structures benefit from the high resolution imaging of laser scanning confocal microscopes. MBF Bioscience and Carl Zeiss have partnered to bring you the most user-friendly and efficient solution for laser scanning confocal stereology.

Stereo Investigator combined with the latest ZEISS LSM confocal microscopes is the world’s most fully integrated system for using stereology to quantify co-labeled objects with the precision of laser scanning confocal microscopy. Additionally, the off-line capabilities of Stereo Investigator workstations permit more effective use of your confocal system by making it available for other users.

Click here to register for Thursday’s webinar.

DHA Supplementation Prior to Brain Injury May Reduce Severity

Helmet, neck roll, shoulder pads, thigh pads, knee pads, mouth guard…  A football player’s list of protective gear goes on and on. New research suggests adding one more item to the list: DHA.

Formally known as docosahexaenoic acid, DHA is one of the human brain’s primary fatty acids. Essential for proper brain function, the omega-3 fatty acid is known to benefit patients with heart disease, cancer, and traumatic brain injuries. Researchers at the West Virginia University School of Medicine say DHA may also help lessen the blow to the brain when taken prior to a head injury.

In their study, the scientists examined the brains of a population of rats, which had received dietary supplementation of DHA for 30 days prior to a traumatic brain injury. They used the Optical Fractionator with Stereo Investigator to quantify the amyloid precursor protein-positive axons, a marker of injury in the brain. A stereological count of injured axons revealed a significantly decreased amount of APP-positive axons in the rats who had received DHA supplements.

In addition to stereological analysis, the researchers assessed the brain damage with immunohistochemistry and water maze testing. Each trial revealed evidence that supplemental DHA was beneficial in reducing the injury response.

“Our findings suggest that meaningful public health benefits are likely from increasing currently low dietary DHA omega-3 intakes in our population overall and, in particular, our at-risk populations,” say the authors.

Read the free abstract or access the full article in Neurosurgery.

Mills, J. D MD; Hadley, K. PhD; Bailes, J. E MD; “Dietary Supplementation With the Omega-3 Fatty Acid Docosahexaenoic Acid in Traumatic Brain Injury” Neurosurgery. 68(2):474-481, February 2011: doi: 10.1227/NEU.0b013e3181ff692b

{Image: Public Domain via Wikipedia}

Q&A: Dr. Daniel Peterson Discusses His Courses on Microscopy and Stereology

Talk of zebrafish and Spaceballs probes make Dr. Daniel Peterson’s microscopy and stereology courses sound intriguing. But Dr. Peterson says it’s the hands-on instruction that students enjoy most about the biannual workshops he teaches in Chicago. From August 15-19, 2011, the Associate Professor and Executive Director at the Center for Stem Cell and Regenerative Medicine at The Rosalind Franklin University of Medicine and Science, welcomes students to Chicago’s Club Quarters for five days of  instruction on all aspects of state-of-the-art microscopy, stereology, and histological analysis.

He spoke to us about the atmosphere in the classroom, what kinds of students take his course, and what aspects of the workshops participants get most excited about. Continue reading “Q&A: Dr. Daniel Peterson Discusses His Courses on Microscopy and Stereology” »

Washington University Researchers use Stereo Investigator to Study Axonal Transport in Parkinson’s Model

Journeying along axons, microscopic powerhouses known as mitochondria provide cells with the energy they need to function. When something goes wrong with the axonal transport and mitochondria isn’t delivered, the system fails, and the cell body dies.

Scientists in the Department of Anatomy and Neurobiology at the Washington University School of Medicine in St. Louis study the cellular bases of neurological disorders. Their recent research focuses on Parkinson’s disease and identifies impaired axonal transport as a possible key player in this neurological disorder.

The research group, of Dr. Karen L. O’Malley, Dr. Jeong Sook Kim-Han, and Dr. Jo Ann Antenor-Dorsey studied axonal transport in dopamine (DA) neurons in the mouse brain—the cells most affiliated with Parkinson’s disease. They localized cell axons by using a microchamber system that separated axons from cell bodies and dendrites. They also introduced 1-methyl-4-phenylpyridinium ion (MPP+), a toxin known to disrupt axonal function and mimic Parkinson’s disease.

Stereo Investigator helped them quantify TH-positive cell bodies and neurites. As early as 12 hours after MPP+ treatment, they saw breaks in axons. After 24 hours, they began to see a significant reduction in cell bodies. “This study underscores the necessity of developing therapeutics aimed at axons as well as cell bodies so as to preserve circuitry and function,” they say in their paper “The Parkinsonian Mimetic, MPP+, Specifically Impairs Mitochondrial Transport in Dopamine Axons, published in the Journal of Neuroscience.

The use of Stereo Investigator saved the researchers time and hard drive space, according to Dr. Kim-Han. “We used to count cells after acquiring images from a fluorescent microscope and saving it to a computer, which took long time and a lot of space on the computer,” she said. “Also, the data was more convincing since the area for counting was selected automatically with the number of fields assigned.”

Read the full paper “The Parkinsonian Mimetic, MPP+, Specifically Impairs Mitochondrial Transport in Dopamine Axons“, in the Journal of Neuroscience.

[The Journal of Neuroscience, 11 May 2011, 31(19): 7212-7221; doi: 10.1523/ JNEUROSCI.0711-11.2011]

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{IMG: Synapse Diagram – This file is licensed under the Creative Commons Attribution-Share Alike 1.0 Generic license}

Stereo Investigator Assists Stanford Stroke Center Scientists in Stem Cell Research

A stroke can leave its victim mentally and physically devastated. Recovery is demanding, and takes drive and determination. If the patient doesn’t receive medical attention within the small, critical window of time after the stroke occurs, chances of a quick recovery are slim. Developments in stem cell research find doctors optimistic about new possibilities for stroke rehabilitation.

Scientists at the Stanford Stroke Center, one of the leading centers for stroke research in the world, are working on figuring out how transplanted stem cells affect the host brain. They’re specifically working toward determining which trophic factors—substances secreted by cells—are necessary for stem cell transplantation to be effective. One recent study focused on vascular endothelial growth factor (VEGF), a factor associated with neurological recovery in stroke patients.

As described in their paper “Transplanted Stem Cell-secreted VEGF Effects Post-stroke Recovery, Inflammation, and Vascular Repair,” Drs. Nobutaka Horie, Gary Steinberg, and their team of researchers found VEGF to be essential for hCNS-SCns-induced recovery. The research team studied the brains of rats, which had undergone stroke surgery and were injected with human central nervous system stem cells (hCNS-SCns). After thorough analysis of various parameters, including stereological quantification of the brain inflammatory response and stem cell survival using Stereo Investigator, the Stanford team determined the stem cells helped suppress inflammation, helped form new blood vessels, and improved blood-brain barrier integrity.”

“Subacute cell transplantation therapy offers a multimodal strategy for brain repair that could significantly expand the therapeutic window for stroke,” say the authors in “Transplanted Stem Cell-secreted VEGF Effects Post-stroke Recovery, Inflammation, and Vascular Repair.”

Access the free abstract or download the full article at Stem Cells.

Horie, N., Pereira, M. P., Niizuma, K., Sun, G., Keren-Gill, H., Encarnacion, A., Shamloo, M., Hamilton, S. A., Jiang, K., Huhn, S., Palmer, T. D., Bliss, T. M. and Steinberg, G. K. (2011), Transplanted Stem Cell-Secreted Vascular Endothelial Growth Factor Effects Poststroke Recovery, Inflammation, and Vascular Repair. STEM CELLS, 29: 274–285. doi: 10.1002/stem.584

{Image of transplanted stem cells (pink) and blood vessels (green) courtesy of Stanford University}

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Neurolucida and Stereo Investigator 10 Available Now

From Alzheimer’s disease to Multiple Sclerosis, genetic disorders, stroke recovery, and stress, Stereo Investigator and Neurolucida are helping scientists around the globe carry out their research. Our flagship products are the leading tools for unbiased stereology and neuron reconstruction. With the release of Version 10, we’re pleased to unveil a whole new set of exceptional features.

Stereo Investigator allows researchers to use unbiased stereology to count cells in a microscope specimen, and to quantify the size and volume of microscopic objects and areas. Neurolucida is the leading software for neuron reconstruction and analysis in labs around the world.

The new Version 10 of both Stereo Investigator and Neurolucida gives researchers numerous new features including:

• The ability to create 3D mosaics from image stacks from confocal, brightfield and fluorescent microscopes

• Integrated deconvolution

• The ability to create 3D focusable slides

• 3D visualization/solid modeling

• Integration with most major confocal microscopes

• Integration with the Zeiss ApoTome 2

Jack Glaser, President and Co-founder of MBF, says “We’re grateful to have a global network of loyal users who give us a constant flow of ideas for improving and expanding our products’ capabilities to match scientists’ evolving research needs. We also use our systems in our own contract research organization, MBF Labs. This gives us first-hand experience using our software, which is another rich source of inspiration. We’ve taken the most powerful ideas from all those sources and developed them into productive features in Version 10, so our users can continue to get the best images, data and analysis possible from their microscopes.”

Get the full list of Stereo Investigator 10 and Neurolucida 10 features on our website, and read our interview with Vice President Paul Angstman to find out about some of his favorite things in Neurolucida 10 and Stereo Investigator 10.