Researchers cited MBF systems in 25 papers between 6/10/2017 and 6/16/2017

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Arawaka, S., Sato, H., Sasaki, A., Koyama, S., & Kato, T. (2017). Mechanisms underlying extensive Ser129-phosphorylation in α-synuclein aggregates. Acta Neuropathologica Communications, 5(1), 48.

Birkl, C., Carassiti, D., Hussain, F., Langkammer, C., Enzinger, C., Fazekas, F., . . . Ropele, S. (2017). Assessment of ferritin content in multiple sclerosis brains using temperature-induced R*2 changes. Magnetic Resonance in Medicine, n/a-n/a. doi: 10.1002/mrm.26780.

Boudewyn, L. C., Sikora, J., Kuchar, L., Ledvinova, J., Grishchuk, Y., Wang, S. L., . . . Walkley, S. U. (2017). N-butyl-deoxynojirimycin delays motor deficits, cerebellar microgliosis, and Purkinje cell loss in a mouse model of mucolipidosis type IV. Neurobiology of Disease. doi: https://doi.org/10.1016/j.nbd.2017.06.003.

Chaves, P. P., Valdoria, C. M., Amorim, M. C. P., & Vasconcelos, R. O. (2017). Ontogenetic development of the inner ear saccule and utricle in the Lusitanian toadfish: Potential implications for auditory sensitivity. Hearing Research. doi: https://doi.org/10.1016/j.heares.2017.06.008.

Ebenezer, G. J., Liu, Y., Judge, D. P., Cunningham, K., Truelove, S., Carter, N. D., . . . Polydefkis, M. (2017). Cutaneous nerve biomarkers in transthyretin familial amyloid polyneuropathy. Annals of Neurology, n/a-n/a. doi: 10.1002/ana.24972.

Fischer, D. L., Kemp, C. J., Cole-Strauss, A., Polinski, N. K., Paumier, K. L., Lipton, J. W., . . . Sortwell, C. E. (2017). Subthalamic Nucleus Deep Brain Stimulation Employs TrkB Signaling for Neuroprotection and Functional Restoration. The Journal of Neuroscience.

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Researchers cited MBF systems in 25 papers between 5/27/2017 and 6/9/2017

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Bastidas, J., Athauda, G., De La Cruz, G., Chan, W.-M., Golshani, R., Berrocal, Y., . . . Pearse, D. D. (2017). Human schwann cells exhibit long-term cell survival, are not tumorigenic and promote repair when transplanted into the contused spinal cord. Glia, n/a-n/a. doi: 10.1002/glia.23161.

Claflin, D. I., Schmidt, K. D., Vallandingham, Z. D., Kraszpulski, M., & Hennessy, M. B. (2017). Influence of postnatal glucocorticoids on hippocampal-dependent learning varies with elevation patterns and administration methods. Neurobiology of Learning and Memory. doi: https://doi.org/10.1016/j.nlm.2017.05.010.

Dioli, C., Patricio, P., Trindade, R., Pinto, L. G., Silva, J. M., Morais, M., . . . Sotiropoulos, I. (2017). Tau-dependent suppression of adult neurogenesis in the stressed hippocampus.  Molecular Psychiatry. doi: 10.1038/mp.2017.103.

Giesert, F., Glasl, L., Zimprich, A., Ernst, L., Piccoli, G., Stautner, C., . . . Wurst, W. (2017). The pathogenic LRRK2 R1441C mutation induces specific deficits modeling the prodromal phase of Parkinson’s disease in the mouse. Neurobiology of Disease. doi: https://doi.org/10.1016/j.nbd.2017.05.013.

Gumusoglu, S. B., Fine, R. S., Murray, S. J., Bittle, J. L., & Stevens, H. E. (2017). The role of IL-6 in neurodevelopment after prenatal stress. Brain, Behavior, and Immunity. doi: https://doi.org/10.1016/j.bbi.2017.05.015.

Issa, J. P. M., Trawitzki, B. F., Ervolino, E., Macedo, A. P., & Lilge, L. (2017). Low-intensity laser therapy efficacy evaluation in FVB mice subjected to acute and chronic arthritis. [journal article]. Lasers in Medical Science, 1-9. doi: 10.1007/s10103-017-2235-5.

Liu, H., Rose, M. E., Ma, X., Culver, S., Dixon, C. E., & Graham, S. H. (2017). In vivo transduction of neurons with TAT-UCH-L1 protects brain against controlled cortical impact injury. Plos one, 12(5), e0178049. doi: 10.1371/journal.pone.0178049.

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A complete guide to imaging and analyzing spines and neurons with Neurolucida 360


Following a well-designed protocol is essential to achieving accurate and consistent results in scientific research. Now, scientists using Neurolucida 360 for dendritic spine and neuron analysis can follow a published set of guidelines to ensure optimal confocal data series for proper dendritic spine quantification and neuron reconstruction. The paper, written by MBF Bioscience scientists and researchers from the Icahn School of Medicine at Mount Sinai in New York, was published in Current Protocols in Neuroscience.

The four protocols describe best practices for imaging and analyzing dendritic spines and entire neurons. Clearly laid out procedures specify necessary materials, image acquisition techniques, and analysis procedures with Neurolucida 360.

Imaging technique is crucial to obtaining unbiased, reproducible results. Clear, crisp images captured with an appropriate z-interval will make analysis with Neurolucida 360 easier and more accurate. Throughout the paper, the authors emphasize the importance of image scaling parameters and unbiased sampling for achieving repeatable results. They also discuss the benefits of correcting optical distortion, especially in the Z-plane, with deconvolution to acquire clear images – a process critical to getting the most accurate representation of dendrites and spines.

Dendritic spine analysis is traditionally performed through tedious, time-consuming manual techniques. According to the paper, this has spawned a growing interest in a more efficient solution for spine quantification and morphological analysis like the one Neurolucida 360 provides. A software platform for automatic neuron reconstruction and spine detection in a 3D environment, Neurolucida 360 offers a variety of benefits, including:

 

  • Fast and accurate spine detection and neuron reconstruction
  • Accurate spine classification and length quantification using a five-point segment that more accurately models the spine backbone.
  • 3 user-guided and automatic algorithms to accurately model neurons visualized with multiple methodologies and imaging techniques.
  • A large number of metrics, including volume, length, and surface area.

 

“We believe that the new quantitative software package, Neurolucida 360, provides the neuroscience research community with the ability to perform higher throughput automated 3D quantitative light microscopy spine analysis under standardized conditions to accelerate the characterization of dendritic spines with greater objectivity and reliability,” (Dickstein, et al. 2016)

The full paper can be found here.

An infographic quickly outlines Protocol 1: Imaging of fluorescently labeled dendritic segments. Use this as a quick reference tool in your lab (right-click on it to save as an image):

Dickstein, D.L., Dickstein, D.R., Janssen, W.G.M., Hof, P.R., Glaser, J.R., Rodriguez, A., O’Connor, N., Angstman, P., and Tappan, S.J. 2016. Automatic dendritic spine quantification from confocal data with Neurolucida 360. Curr. Protoc. Neurosci. 77:1.27.1-1.27.21. doi: 10.1002/cpns.16

Researchers cited MBF systems in 18 papers during the week of 12/12/2016

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Alberico, S. L., Kim, Y.-C., Lence, T., & Narayanan, N. S. (2016). Axial levodopa-induced dyskinesias and neuronal activity in the dorsal striatum. Neuroscience. doi: http://dx.doi.org/10.1016/j.neuroscience.2016.11.046.

Forgione, N., Chamankhah, M., & Fehlings, M. (2016). A Mouse Model of Bilateral Cervical Contusion-Compression Spinal Cord Injury. Journal of Neurotrauma. doi: 10.1089/neu.2016.4708.

Gasser, E., Johannssen, H. C., Rülicke, T., Zeilhofer, H. U., & Stoffel, M. (2016). Foxa1 is essential for development and functional integrity of the subthalamic nucleus. Scientific Reports, 6, 38611. doi: 10.1038/srep38611

Ghosh, A., Tyson, T., George, S., Hildebrandt, E. N., Steiner, J. A., Madaj, Z., . . . Brundin, P. (2016). Mitochondrial pyruvate carrier regulates autophagy, inflammation, and neurodegeneration in experimental models of Parkinson’s disease. [10.1126/scitranslmed.aag2210]. Science translational medicine, 8(368), 368ra174.

Jacinto, L. R., Mata, R., Novais, A., Marques, F., & Sousa, N. (2016). The habenula as a critical node in chronic stress-related anxiety. Experimental Neurology. doi: http://dx.doi.org/10.1016/j.expneurol.2016.12.003.

Jobe, E. M., Gao, Y., Eisinger, B. E., Mladucky, J. K., Giuliani, C. C., Kelnhofer, L. E., & Zhao, X. (2016). Methyl-CpG binding protein MBD1 regulates neuronal lineage commitment through maintaining adult neural stem cell identity. [10.1523/JNEUROSCI.1075-16.2016]. The Journal of Neuroscience.

Mandolesi, G., De Vito, F., Musella, A., Gentile, A., Bullitta, S., Fresegna, D., . . . Mori, F. (2016). miR-142-3p is a key regulator of IL-1β-dependent synaptopathy in neuroinflammation. Journal of Neuroscience, 0851-0816.

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Researchers cited MBF systems in 16 papers between 11/1/2016 and 11/11/2016

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Bode, C., Richter, F., Spröte, C., Brigadski, T., Bauer, A., Fietz, S., . . . Richter, A. (2017). Altered postnatal maturation of striatal GABAergic interneurons in a phenotypic animal model of dystonia. Experimental Neurology, 287, Part 1, 44-53. doi: http://dx.doi.org/10.1016/j.expneurol.2016.10.013.

Calvez, J., de Ávila, C., Guèvremont, G., & Timofeeva, E. (2016). Sex-specific effects of chronic administration of relaxin-3 on food intake, body weight and hypothalamo-pituitary-gonadal axis in rats. Journal of Neuroendocrinology, n/a-n/a. doi: 10.1111/jne.12439.

Haight, J. L., Fuller, Z. L., Fraser, K. M., & Flagel, S. B. (2016). A food-predictive cue attributed with incentive salience engages subcortical afferents and efferents of the paraventricular nucleus of the thalamus. Neuroscience. doi: http://dx.doi.org/10.1016/j.neuroscience.2016.10.043.

He, H., Guo, W.-W., Xu, R.-R., Chen, X.-Q., Zhang, N., Wu, X., & Wang, X.-M. (2016). Alkaloids from piper longum protect dopaminergic neurons against inflammation-mediated damage induced by intranigral injection of lipopolysaccharide. BMC Complementary and Alternative Medicine, 16(1), 412. doi: 10.1186/s12906-016-1392-6.

Jin, T., Nicholls, F. J., Crum, W. R., Ghuman, H., Badylak, S. F., & Modo, M. (2017). Diamagnetic chemical exchange saturation transfer (diaCEST) affords magnetic resonance imaging of extracellular matrix hydrogel implantation in a rat model of stroke. Biomaterials, 113, 176-190. doi: http://dx.doi.org/10.1016/j.biomaterials.2016.10.043.

Kyyriäinen, J., Ekolle Ndode-Ekane, X., & Pitkänen, A. (2016). Dynamics of PDGFRβ expression in different cell types after brain injury. Glia, n/a-n/a. doi: 10.1002/glia.23094.

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Researchers cited MBF systems in 15 papers during the week of 10/24/2016

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Casaca-Carreira, J., Temel, Y., Larrakoetxea, I., & Jahanshahi, A. (2016). Distribution and Penetration of Intracerebroventricularly Administered 2′OMePS Oligonucleotide in the Mouse Brain. Nucleic Acid Therapeutics. doi: 10.1089/nat.2016.0642.

Guatteo, E., Rizzo, F. R., Federici, M., Cordella, A., Ledonne, A., Latini, L., . . . Mercuri, N. B. (2017). Functional alterations of the dopaminergic and glutamatergic systems in spontaneous α-synuclein overexpressing rats. Experimental Neurology, 287, Part 1, 21-33.

Gupta, R., Saini, S., Sharma, S., Jacob, T. G., & Roy, T. S. (2017). Morphology of the Human Pancreas During Development and Aging. In P. C. Rath, R. Sharma & S. Prasad (Eds.), Topics in Biomedical Gerontology (pp. 67-89). Singapore: Springer Singapore.

Hook, M., Woller, S., Bancroft, E., Aceves, M., Funk, M. K., Hartman, J., & Garraway, S. M. (2016). Neurobiological effects of Morphine following Spinal Cord Injury. Journal of Neurotrauma. doi: 10.1089/neu.2016.4507. http://dx.doi.org/10.1089/neu.2016.4507

Schaeffer, E. L., Catanozi, S., West, M. J., & Gattaz, W. F. (2016). Stereological investigation of the CA1 pyramidal cell layer in untreated and lithium-treated 3xTg-AD and wild-type mice. Annals of Anatomy – Anatomischer Anzeiger. doi: http://dx.doi.org/10.1016/j.aanat.2016.10.002.

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Researchers cited MBF systems in 14 papers during the week of 10/17/2016

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Bacigaluppi, M., Russo, G. L., Peruzzotti-Jametti, L., Rossi, S., Sandrone, S., Butti, E., . . . Martino, G. (2016). Neural Stem Cell Transplantation Induces Stroke Recovery by Upregulating Glutamate Transporter GLT-1 in Astrocytes.  The Journal of Neuroscience, 36(41), 10529.

Beck, M. H., Haumesser, J. K., Kühn, J., Altschüler, J., Kühn, A. A., & van Riesen, C. (2016). Short- and long-term dopamine depletion causes enhanced beta oscillations in the cortico-basal ganglia loop of parkinsonian rats. Experimental Neurology, 286, 124-136. doi: http://dx.doi.org/10.1016/j.expneurol.2016.10.005.

Huang, C.-W., Chen, Y.-W., Lin, Y.-R., Chen, P.-H., Chou, M.-H., Lee, L.-J., . . . Chen, S.-L. (2016). Conditional Knockout of Breast Carcinoma Amplified Sequence 2 (BCAS2) in Mouse Forebrain Causes Dendritic Malformation via β-catenin.  Scientific Reports, 6, 34927. doi: 10.1038/srep34927

Sack, M., Lenz, J. N., Jakovcevski, M., Biedermann, S. V., Falfán-Melgoza, C., Deussing, J., . . . Auer, M. K. (2016). Early effects of a high-caloric diet and physical exercise on brain volumetry and behavior: a combined MRI and histology study in mice. Brain Imaging and Behavior, 1-12. doi: 10.1007/s11682-016-9638-y.

Schelle, J., Häsler, L., Göpfert, J. C., Joos, T. O., Vanderstichele, H., Stoops, E., . . . Kaeser, S. A. (2016). Prevention of tau increase in cerebrospinal fluid of APP transgenic mice suggests downstream effect of BACE1 inhibition. Alzheimer’s & Dementia. doi: http://dx.doi.org/10.1016/j.jalz.2016.09.005.

Teo, J. D., Morris, M. J., & Jones, N. M. (2016). Maternal obesity increases inflammation and exacerbates damage following neonatal hypoxic-ischaemic brain injury in rats. Brain, Behavior, and Immunity. doi:http://dx.doi.org/10.1016/j.bbi.2016.10.010.

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Researchers cited MBF systems in 22 papers during the week of 10/10/2016

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Acosta, S. A., Tajiri, N., Sanberg, P. R., Kaneko, Y., & Borlongan, C. V. (2016). Increased Amyloid Precursor Protein and Tau Expression Manifests as Key Secondary Cell Death in Chronic Traumatic Brain Injury. Journal of Cellular Physiology, n/a-n/a. doi: 10.1002/jcp.25629.

Chan, A., Li, S., Lee, A. R., Leung, J., Yip, A., Bird, J., . . . Pompeiano, M. (2016). Activation of state-regulating neurochemical systems in newborn and embryonic chicks. Neuroscience. doi:http://dx.doi.org/10.1016/j.neuroscience.2016.09.048.

Gomez-Smith, M., Karthikeyan, S., Jeffers, M. S., Janik, R., Thomason, L. A., Stefanovic, B., & Corbett, D. (2016). A physiological characterization of the Cafeteria diet model of metabolic syndrome in the rat. Physiology and Behavior. doi: http://dx.doi.org/10.1016/j.physbeh.2016.09.029.

Lei, B., James, M. L., Liu, J., Zhou, G., Venkatraman, T. N., Lascola, C. D., . . . Wang, H. (2016). Neuroprotective pentapeptide CN-105 improves functional and histological outcomes in a murine model of intracerebral hemorrhage. Scientific Reports, 6, 34834. doi: 10.1038/srep34834.

Li, Y. Q., Cheng, Z.-C., Liu, S.-W., Aubert, I., & Wong, C. S. (2016). P53 regulates disruption of neuronal development in the adult hippocampus after irradiation.  Cell Death Discovery, 2, 16072. doi: 10.1038/cddiscovery.2016.72

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Researchers cited MBF systems in 21 papers during the week of 10/3/2016

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Augur, I. F., Wyckoff, A. R., Aston-Jones, G., Kalivas, P. W., & Peters, J. (2016). Chemogenetic Activation of an Extinction Neural Circuit Reduces Cue-Induced Reinstatement of Cocaine Seeking. The Journal of Neuroscience, 36(39), 10174-10180.

Bocchio, M., Fisher, S. P., Unal, G., Ellender, T. J., Vyazovskiy, V. V., & Capogna, M. (2016). Sleep and serotonin modulate paracapsular nitric oxide synthase expressing neurons of the amygdale. [10.1523/ENEURO.0177-16.2016]. eneuro.

Cope, E. C., Briones, B. A., Brockett, A. T., Martinez, S., Vigneron, P.-A., Opendak, M., . . . Gould, E. (2016). Immature neurons and radial glia, but not astrocytes or microglia, are altered in adult Cntnap2 and Shank3 mice, models of autism. [10.1523/ENEURO.0196-16.2016]. eneuro.

Dela Cruz, J. A. D., Coke, T., & Bodnar, R. J. (2016). Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats. (114), e53897. doi: doi:10.3791/53897.

Dixon, K. J., Mier, J., Gajavelli, S., Turbic, A., Bullock, R., Turnley, A. M., & Liebl, D. J. (2016). EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury. Stem Cell Research. doi:http://dx.doi.org/10.1016/j.scr.2016.09.029.

Fahimi, A., Baktir, M. A., Moghadam, S., Mojabi, F. S., Sumanth, K., McNerney, M. W., . . . Salehi, A. (2016). Physical exercise induces structural alterations in the hippocampal astrocytes: exploring the role of BDNF-TrkB signaling. Brain Structure and Function, 1-12. doi: 10.1007/s00429-016-1308-8.

Fernandez-Gonzalez, P., Benavides-Piccione, R., Leguey, I., Bielza, C., Larrañaga, P., & DeFelipe, J. (2016). Dendritic-branching angles of pyramidal neurons of the human cerebral cortex. Brain Structure and Function, 1-13. doi: 10.1007/s00429-016-1311-0.  Continue reading “Researchers cited MBF systems in 21 papers during the week of 10/3/2016” »

Researchers cited MBF systems in 17 papers during the week of 9/26/2016

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Biedermann, S. V., Auer, M. K., Bindila, L., Ende, G., Lutz, B., Weber-Fahr, W., . . . Fuss, J. (2016). Restricted vs. unrestricted wheel running in mice: Effects on brain, behavior and endocannabinoids. Hormones and Behavior, 86, 45-54. doi: http://dx.doi.org/10.1016/j.yhbeh.2016.09.007.

Choi, E. Y., Tanimura, Y., Vage, P. R., Yates, E. H., & Haber, S. N. (2016). Convergence of prefrontal and parietal anatomical projections in a connectional hub in the striatum. Neuroimage. doi:http://dx.doi.org/10.1016/j.neuroimage.2016.09.037.

García-Cabezas, M. Á., & Barbas, H. (2016). Anterior Cingulate Pathways May Affect Emotions Through Orbitofrontal Cortex. Cerebral Cortex. doi: 10.1093/cercor/bhw284.

Gomez, A. M., Stevens, J. A. A., Mané-Damas, M., Molenaar, P., Duimel, H., Verheyen, F., . . . Martinez-Martinez, P. (2016). Silencing of Dok-7 in Adult Rat Muscle Increases Susceptibility to Passive Transfer Myasthenia Gravis. The American journal of pathology, 186(10), 2559-2568. doi: http://dx.doi.org/10.1016/j.ajpath.2016.05.025.

Halici, Z., Polat, B., Cadirci, E., Topcu, A., Karakus, E., Kose, D., . . . Bayir, Y. (2016). Inhibiting renin angiotensin system in rate limiting step by aliskiren as a new approach for preventing indomethacin induced gastric ulcers. Chemico-Biological Interactions, 258, 266-275. doi: http://dx.doi.org/10.1016/j.cbi.2016.09.011.

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