Cox J, Pinto L, Dan Y.
Cell-type-specific calcium imaging revealed distinct and spatially organized activity patterns in the dorsal pons across sleep–wake states. Glutamatergic neurons were predominantly active during REM sleep, while GABAergic neurons were biased toward wakefulness, highlighting specialized subpopulations involved in sleep regulation.... Read More
Yang W, Miller JE, Carrillo-Reid L, Pnevmatikakis E, Paninski L, Yuste R, Peterka DS.
A holographic two-photon imaging system enables simultaneous multi-plane recording of neuronal activity across cortical layers with single-cell precision. Multiplexed excitation and synchronized acquisition capture fast, correlated calcium dynamics beyond the limits of sequential two-photon scanning.... Read More
Zhao S, Hebert E, Gruzdeva A, Mahishi D, Takahashi H, Lee S, Hao YA, Lin MZ, Yapici N, Xu C.
Adaptive excitation enables high-speed, deep two-photon voltage imaging of multiple neurons while staying below tissue heating limits. By boosting signal collection and supporting simultaneous dual-plane recordings, this approach captures rapid neuronal activity at depths and speeds not achievable with conventional two-photon microscopy.... Read More
Mok AT, Wang T, Zhao S, Kolkman KE, Wu D, Ouzounov DG, Seo C, Wu C, Fetcho JR, Xu C.
An integrated multiphoton system enabled large field-of-view, single-cell imaging deep into cortical and subcortical regions. Simultaneous two- and three-photon imaging captured activity across thousands of neurons, extending depth and coverage beyond conventional approaches.... Read More
Yu CH, Stirman JN, Yu Y, Hira R, Smith SL.
A dual-engine two-photon mesoscope enabled high-resolution, simultaneous imaging across widely separated cortical regions. Independent scan control and adaptive optics supported flexible, large-scale recording of thousands of neurons in vivo, overcoming traditional field-of-view limitations in two-photon microscopy.... Read More
Pologruto TA, Sabatini BL, Svoboda K.
ScanImage enables real-time control of laser scanning microscopes using standard data acquisition hardware, eliminating the need for complex, custom electronics. It delivers high-quality, multi-channel imaging with microsecond-level precision while offering the flexibility researchers need to build and adapt advanced confocal and two-photon microscopy systems.... Read More