MBF Webinars


SRS Image Stack Acquisition


DATE: Wednesday, June 30, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Jose Maldonado, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
Stereo Investigator lets you automatically acquire a series of image stacks using Systematic Random Sampling (SRS) for offline, unbiased stereological analysis. The offline analysis can be done after acquisition on a computer running Stereo Investigator Workstation software. There are several advantages to using this technique:

   1. Fluorescent specimens can be quickly imaged and later analyzed, thus reducing the effect of photobleaching.
   2. The microscopy hardware is available for other uses during the offline analysis.

This webinar will demonstrate the use of the Acquire SRS Image Stack Workflow in Stereo Investigator. We will acquire a series of image stacks suitable for use in offline Optical Fractionator analysis. Users can expect to learn the basic techniques needed to acquire image stacks for offline analysis, as well as tips on how to optimize imaging and acquisition settings.

WHAT YOU’LL LEARN:
You will learn how to use the Acquire SRS Image Stack Workflow to generate an image stack series suitable for offline, unbiased cell counting with Optical Fractionator.

VIEW WEBINAR:
Click here to view the webinar.




Automated Neuron Reconstruction with AutoNeuron


DATE: Thursday, April 29, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
In this practical demonstration, Dr. Susan Hendricks will use the AutoNeuron extension module for Neurolucida to automatically reconstruct a labeled neuron. Using the workflow, she will work with a 3D image stack containing a filled neuron imaged with a confocal microscope to create a three dimensional model of the labeled cell. She will demonstrate automatic soma detection, automatic and interactive branch reconstruction, and how to display both the image data and trace model in 3D for visualization. This webinar will serve as a precursor to the Advanced Techniques for AutoNeuron webinar.

WHAT YOU’LL LEARN:
This presentation will demonstrate how to use the AutoNeuron extension module for Neurolucida to create 3D reconstruction of a labeled cell automatically.

VIEW WEBINAR:
Click here to view the webinar.




Stereological Techniques for Area and Volume Estimation

DATE: Wednesday, March 24, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
In this practical demonstration, Dr. Susan Hendricks uses Neurolucida 9 to demonstrate manual neuron reconstruction techniques on Golgi-stained tissue sections. Dr. Hendricks will show you how to apply the different methods Neurolucida 9 uses to display your data, including macro view, orthogonal view, and various display settings to improve your accuracy and efficiency. We’ll also look at using Neurolucida Explorer to export data and perform data analysis.

WHAT YOU’LL LEARN:
This presentation will demonstrate how to use Stereo Investigator to estimate area and volume of regions of interest from histological sections, images, and contours.






Practical Demonstration of Neuron Reconstruction with Neurolucida

DATE: Wednesday, February 24, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
In this practical demonstration, Dr. Susan Hendricks uses Neurolucida 9 to demonstrate manual neuron reconstruction techniques on Golgi-stained tissue sections. Dr. Hendricks will show you how to apply the different methods Neurolucida 9 uses to display your data, including macro view, orthogonal view, and various display settings to improve your accuracy and efficiency. We’ll also look at using Neurolucida Explorer to export data and perform data analysis.

WHAT YOU’LL LEARN:
This presentation will demonstrate how to create a reconstruction of Golgi stained neurons using Neurolucida 9 and a Zeiss Axio Imager Z1 motorized microscope.

VIEW WEBINAR:
Click here to view the Neuron Reconstruction with Neurolucida Webinar.





Practical Demonstration of the Optical Fractionator Workflow

DATE: Wednesday, January 27, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
The Optical Fractionator is a design-based stereology method for unbiased determination of cell populations. Dr. Susan Hendricks will demonstrate the step-by-step implementation of the Optical Fractionator Workflow within Stereo Investigator. Using a Zeiss brightfield microscope controlled by Stereo Investigator, Susan will demonstrate how to set-up and count huntingtin aggregates live, fielding your questions along the way. Aspects of data collection and reporting will be discussed as well as tips to improve your efficiency.

WHAT YOU’LL LEARN:
This presentation will demonstrate efficient use of the Optical Fractionator Workflow for cell population estimation using Stereo Investigator.

VIEW WEBINAR:
Click here to view the Optical Fractionator Workflow Webinar.




An Introduction to Experimental Design for Stereological Estimation of Cell Number

DATE: Wednesday, September 9, 2009
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
Design based stereology is a powerful method for unbiased determination of cell population estimates. To achieve accuracy and efficiency during the application of stereological methods, sampling design must be considered at the outset of the study. We will discuss the impact of systematic random sampling, how to implement the optical fractionator principle and the importance of histological techniques. This presentation will serve as the first in a series of practical applications of stereology theory.

WHAT YOU’LL LEARN:
This presentation will highlight experimental design considerations for efficient cell population estimates using Stereo Investigator.