MBF Webinars




Stereological Techniques for Area and Volume Estimation

DATE: Wednesday, March 24, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
In this practical demonstration, Dr. Susan Hendricks uses Neurolucida 9 to demonstrate manual neuron reconstruction techniques on Golgi-stained tissue sections. Dr. Hendricks will show you how to apply the different methods Neurolucida 9 uses to display your data, including macro view, orthogonal view, and various display settings to improve your accuracy and efficiency. We’ll also look at using Neurolucida Explorer to export data and perform data analysis.

WHAT YOU’LL LEARN:
This presentation will demonstrate how to use Stereo Investigator to estimate area and volume of regions of interest from histological sections, images, and contours.

Reserve your webinar space now at:
ww2.gotomeeting.com

System Requirements:

  • Microsoft Windows® 2000, Windows XP Home/Pro, Windows 2003 Server, Windows Vista®
  • Mac OS® X 10.4 Tiger® or newer






Practical Demonstration of Neuron Reconstruction with Neurolucida

DATE: Wednesday, February 24, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
In this practical demonstration, Dr. Susan Hendricks uses Neurolucida 9 to demonstrate manual neuron reconstruction techniques on Golgi-stained tissue sections. Dr. Hendricks will show you how to apply the different methods Neurolucida 9 uses to display your data, including macro view, orthogonal view, and various display settings to improve your accuracy and efficiency. We’ll also look at using Neurolucida Explorer to export data and perform data analysis.

WHAT YOU’LL LEARN:
This presentation will demonstrate how to create a reconstruction of Golgi stained neurons using Neurolucida 9 and a Zeiss Axio Imager Z1 motorized microscope.

VIEW WEBINAR:
Click here to view the Neuron Reconstruction with Neurolucida Webinar.





Practical Demonstration of the Optical Fractionator Workflow

DATE: Wednesday, January 27, 2010
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
The Optical Fractionator is a design-based stereology method for unbiased determination of cell populations. Dr. Susan Hendricks will demonstrate the step-by-step implementation of the Optical Fractionator Workflow within Stereo Investigator. Using a Zeiss brightfield microscope controlled by Stereo Investigator, Susan will demonstrate how to set-up and count huntingtin aggregates live, fielding your questions along the way. Aspects of data collection and reporting will be discussed as well as tips to improve your efficiency.

WHAT YOU’LL LEARN:
This presentation will demonstrate efficient use of the Optical Fractionator Workflow for cell population estimation using Stereo Investigator.

VIEW WEBINAR:
Click here to view the Optical Fractionator Workflow Webinar.




An Introduction to Experimental Design for Stereological Estimation of Cell Number

DATE: Wednesday, September 9, 2009
TIME: 3:00 PM EST [12:00 PM PST]

SPEAKER: Susan Hendricks, Ph.D., Staff Scientist, MBF Bioscience

ABSTRACT:
Design based stereology is a powerful method for unbiased determination of cell population estimates. To achieve accuracy and efficiency during the application of stereological methods, sampling design must be considered at the outset of the study. We will discuss the impact of systematic random sampling, how to implement the optical fractionator principle and the importance of histological techniques. This presentation will serve as the first in a series of practical applications of stereology theory.

WHAT YOU’LL LEARN:
This presentation will highlight experimental design considerations for efficient cell population estimates using Stereo Investigator.