Maximum intensity projection

Purpose

To visualize an entire image stack in a single plane, use Maximum intensity projection. To display the projection, BrightSLICE software identifies the maximum intensity value of each pixel along the z axis for projection onto the 2D plane.

See Deep Focus for a similar, alternative method.

Procedure

Displaying Min or Max Projection

  • Click Min (or Max) projectionon the Image ribbon to display the projection rather than the original image.
  • To view the original image stack again, Min (or Max) projection again.

If you perform any image adjustment on a projection, you'll need to toggle the projection off and then back on in order to refresh the projection image.

Saving a Max Projection

You can save the projection image using either of the following equivalent methods:

  1. Go to File > Save As > Image

    Go to File > Save As > Image Stack > Current Plane

    You will get a warning message that you are saving only one image of the image stack; that's exactly what you want to do when saving a Max Projection, so click OK to close the warning window.

  2. In the Save As window that opens, indicate the desired file name and location, then click Save.