Maximum intensity projection

Purpose

To visualize an entire image stack in a single plane, use Maximum intensity projection. To display the projection, BrightSLICE software identifies the maximum intensity value of each pixel along the z axis for projection onto the 2D plane.

See Deep Focus for a similar, alternative method.

Procedure

The Max Projection button toggles the maximum-projection effect on and off.

• Click the Max projection button, located in the View section of the Image ribbon, to display the projection rather than the original image.
• To view the original image stack again, click the button again.

If you perform any image adjustment on a projection, you'll need to toggle the projection off and then back on in order to refresh the projection image.

Saving a Max Projection

You can save the projection image using either of the following equivalent methods:

1. Go to File > Save As > Image

   Go to File > Save As > Image Stack > Current Plane

   You will get a warning message that you are saving only one image of the image stack; that's exactly what you want to do when saving a Max Projection, so click ok to close the warning window.

2. In the Save As window that opens, indicate the desired file name and location, then click Save.