Live crop

Purpose

Live crop is used to exclude areas of vignetting and image distortion from the edges of the field of view. To access and set up Live crop, click More Settings on the Camera Settings panel , then click Configure Crop in the Binning and Cropping tab. For assistance with camera-specific settings, contact our Technical Services staff.

Procedure

  1. Turn on Live Image in the Acquire ribbon

  2. Click More Settings in the Camera Settings panel.

  3. Click Configure Crop in the Binning and Cropping tab to open the Live Crop window.

  4. Adjust the size and position of white dashed box in the viewing area.

    1. Using a clear structure in the tissue sample, or the fluorescent background, determine the cropping area.

    2. Exclude all dark corners or and/or distorted edges in the field of view from the cropping area.

      1. Click and drag the edges or corners of the white dashed box to resize the crop.

      2. Click inside the white dashed box and drag the mouse to move the crop in X and Y.

    3. (Optional) Click Fit To Window in Live Crop window to stretch the crop to fill the entire viewing area.

    4. Click Accept Crop in Live Crop window.

    5. Click OK in the More Settings window.

Live crop settings

Live crop can be accessed in the Binning and Cropping tab of the More Settings window.

Live crop: Use live crop to exclude areas of vignetting and image distortion from the edges of the field of view. You can manually enter the desired size and position values, but we recommend using the Configure Crop tool to set the size and position of the live crop.

  • X Centering: How many pixels the crop has been shifted in the X axis to center it in the viewing window.

  • Y Centering: How many pixels the crop has been shifted in the Y axis to center it in the viewing window.

  • Width: The width of the live crop in pixels.

  • Height: The height of the live crop in pixels.

  • Apply Crop Click to apply crop size and position values manually entered into the fields above.

  • Clear Crop Click to clear an active live crop. The cropping will be removed and the viewing area will display the entire field of view from the camera feed.

    Note that clearing a crop cannot be undone. To apply a live crop again, the crop will need to be reconfigured or position and size values will need to be manually entered and applied.

  • Configure Crop Click to configure the size and position of the crop.