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Calibrating your system

A properly calibrated system is essential for successful and efficient software use. Follow our guidelines carefully to ensure proper system function.

Watch our videos on YouTube: Calibrating lenses with calibration grids (4:34), Parcentric/parfocal calibration (2:47), Checking calibration accuracy (1:27).

Equipment

• A microscope system (camera, stage, objectives, computer etc.)
• Calibration grid slide
• The latest version of MBF Bioscience software
• Optional: Allen wrenches for aligning camera and Stage.

Before you start...

1. Turn on the microscope system and the computer.
2. Start Microlucida.
3. Load the calibration grid slide onto the microscope.

Overview of the calibration process

1. Add and tighten the objectives:
   • Tighten all the objectives by hand to make sure they have not come loose.
   • Add any new objectives to the microscope BEFORE calibrating the system.
2. Verify that camera and stage are aligned.
3. Calibrate lenses with calibration grids.
4. Perform parcentric/parfocal calibration.
5. Check the accuracy of the calibration.

Refer to the Troubleshooting section if necessary.

See/Print PDF

Also see Creating a Lens, Creating a New Lens from an Image of a Calibration Grid Slide, Edit Lens

Microlucida 3 | MBF Bioscience Support Center | Downloads