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Serial Section Reconstruction from Glass Slides

Use these instructions if you have one section per slide.

 

  1. Load the first slide on the stage.
  2. Open the Serial Section Manager (Tools>Serial Section Manager).
    1.  Select Show Current Section Only and Show Suppressed as Gray
    2. Click the New Section icon to create as many sections as needed for the reconstruction.
      • The number of sections created corresponds to the number of sections to be traced.
    3. Highlight the first section.
  3. Select Move>Joy Free.
  4. Disable Joy Free: Right-click and select End Joy Free.
  5. In the Tracing window, trace a contour in the section using one of the methods below. You may want to trace fiducial contours.
  6. Go to the next section to be traced:
    1. Use Go To to move the stage to a portion of the contour that is helpful for aligning to the next section.
    2. Load the slide with the next section on the stage.
    3. Select Move>Joy Free.
    4. Move the stage to bring the same relative location on the new section into view.
    5. Disable Joy Free.
    6. Make sure the contour you just traced is not grayed out. See details in Serial Section Manager: Buttons>Show Suppressed as Gray.
      • If it is grayed out: In the Serial Section Manager, select the section above the section currently selected (in other words, select the section in which you just traced the contour instead of the section containing the tissue to be traced) and verify that the contour is colored.
  7. Align the already traced contour and the image of the section to be traced.
  8. In the Serial Section Manager, select the next section.
  9. Trace the contour using your preferred method.
  10. Repeat steps 6 through 9 for the next sections to be traced.
  11. Visualize the reconstruction in 3D.
    1. In the Serial Section Manager, click Display Current Section Only to disable it; you should see all the contours in the chosen contour color.
    2. Click the 3D Visualization icon .
    3. Select Surface Reconstruction from the Contours options.
    4. Rotate the reconstruction.

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