Outline detected objects

Watch a demonstration on our YouTube channel.

This detection algorithm is based on the current image adjustments histogram, not the raw image data.

Open an image (you can't detect on a live image).
Zoom to an area containing objects representative of the objects you wish to outline.
Click the Outline Detected Objects icon to display the Outline Detected Objects window.
Select Use Full Image or Use Region(s) of Interest under Detection Space.
Use Region(s) of Interest
- Click Use Existing ROIs to use existing contours.
  - Click a contour to select it; hold down Shift and click on contours to select multiple contours.
  - Click Done Selecting when satisfied with your selection.
- Click Draw ROIs to trace a contour.
  - Trace a contour in the Tracing window.
  - Click Finished ROIs.
Click a representative object. Microlucida displays a preview of outlined detected objects.
- For a list of contour measurements, click the Contour Measurements icon to the right of Results. Microlucida displays the results in the Contour Measurements window.
- If the outline is difficult to distinguish, change its color: Under Status / Change Preview Color, click the icon to change the color of the outline.
- To use presets : See Automatic Object Detection/Presets.
Adjust any of the parameters; every time you adjust a parameter, Microlucida updates the Results .
Parameters
- Sensitivity: The higher the sensitivity, the smaller the detected object around the representative sample color (displayed under Object Color ).
  - Click Apply to refresh the preview.
  - To sample a different color, click on another color in the tissue.
- Outline Point Density: The higher the density , the more precise the outline. Click Apply to refresh the preview.
- Exclude objects: Excludes objects from detection that are Smaller than/ Larger than the set micron value; use in conjunction with sensitivity.
- Separate Objects By Average Size: Use to increase the accuracy for the count of detected objects.
Example: You define the average size as 10 µm2 and Microlucida detects an object approximately 30 µm2.
The program counts the object as 3 objects instead of 1.
- Screen Edge Restrictions:Microlucida ignores objects that meet the criteria.
Click Outline Objects to apply the parameters.