Image Registration Panel

See Adjusting image alignment for detailed procedures.

To open the Image Registration panel, click the registration button in the Workflow section of the ribbon.

Use this panel if:

  • You're not satisfied with the automatic alignment/registration of your stacks
  • You want to check the automatic alignment/registration of your stacks
  • You want to manually align/register your stacks

Settings

Reference

The image used as the reference for registering all subsequent images.

Displays the label (Image File Name, Elapsed Time, or Time Point Name) selected in the Show Label As: section of the Image selection panel.

Image | Fit | Alignment table

  • Image: Lists all images other than the reference image.
  • Fit: Quality of the alignment between reference image and image. 70+ is good
  • Alignment: Method used for image alignment, Automatic (when loading images), Refined or Manual

Automatic alignment

Reverts to the original alignment.

  1. Click Manual alignment.
  2. Click to place one marker at a landmark site in the left image then place a marker in the right image at the same landmark site; repeat for two additional landmark sites.
  3. Click Done.
  4. Review the results of the manual alignment: Click Check alignment, place a fourth marker at a unique landmark site in the left image and verify that the marker is displayed at the same landmark site in the right image.

The user input for the refined alignment method is the same as that for the manual method, however, the software uses additional information about your image to refine the alignment.

Use to check the accuracy of your image alignment; if image alignment does not meet your needs, repeat the alignment process.