Batch trace workflow

With Batch Trace, you can trace over multiple images using a single Neurolucida 360 configuration. The program generates an .ASC or .DAT data file for each image processed. The following file formats can be processed:

Access the workflow from Trace>Automatic>Batch Trace.

The images to be processed must be set to the same µm/px scale and X/Y, and to the same Z step (for 3D images).

Prepare for the batch run

1. In the 3D window, open an image stack representative of the image stacks you'll process with the batch run.
2. Detect somas, trace with the automatic method, detect varicosities, spines and synapses as needed.
3. Click the Settings button to save the settings used in Step 2. They will be used for the batch run.
4. In the 3D Settings window, click Save current settings and enter a name for the configuration.
5. Close the 3D window. You don't need to save the data file.
6. In the standard window, go to the Trace ribbon and click Automatic>Batch trace to start the workflow.