Open topic with navigation

Virtual Tissue (slide scanning) overview

The Virtual Tissue module (2D or 3D) is used to acquire high-resolution images of slides and tissues.

• A motorized stage is used to acquire a series of individual image tiles. The individual tiles are merged to form a single image montage called a virtual slide.
• Available for single image planes (2D acquisition) and for multiple planes (creating a 3D virtual image stack).
• Virtual slides can be acquired at any magnification.

Before you start, make sure that you have a properly calibrated system to obtain high-quality images.

Procedure

1. Optimizing your workspace for the acquisition
2. Tracing the region of interest
3. Camera (& multichannel) settings
4. Acquiring a background image (Brightfield only)
5. Acquisition settings
6. Setting up a focus map (2D and 3D images)
7. Acquiring & compiling the image

Also see Troubleshooting (brightfield) or Troubleshooting (fluorescence)

View/print a PDF about the procedure.

Watch a video on Whole Slide Imaging to learn more about Virtual Tissue.

Microlucida 3 | MBF Bioscience Support Center | Downloads