Weibel

	Surface area / volume		Use Weibel to estimate the ratio of length to cross-sectional area and surface area to volume. This method uses a linear test system devised by Weibel. The probe displays a set of short lines laid out in a grid over the material to be examined. Markers are placed wherever the test lines cross the boundaries of the surface.
	Thin
	Isotropic sections

 

Prerequisites

• IUR sections or isotropically shaped objects
• If objects are not isotropically shaped, some of them must be isotropically oriented or observed in several isotropic sections.

How do use the Weibel probe?

1. Click Probes>Weibel. The Lines Coherent Test System dialog box appears .
2. Specify the length of the test lines. Based on the length value, Stereo Investigator determines the distance from the end of each line to the beginning of its nearest neighbors.
   • Experiment to determine an appropriate grid size in order to obtain a statistically significant number of intersections.
3. Select a marker.
4. Mark each intersection of an object’s boundary with the grid.
6. When finished, click Probes>Weibel to exit the probe.
7. To view results, click Probes>Display Probe Run List.

 

Formulas & references...

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Stereo Investigator 11 | MBF Bioscience Support Center | Downloads