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Virtual Tissue (slide scanning): Camera (& multichannel) settings

Now that the regions of interest are traced, the third step is to adjust the camera settings (as well as the multichannel settings if you are using fluorescence).

 

ClosedSettings for Brightfield
  1. Perform a white balance to correct for any coloring imbalances.
    • Find an area on the tissue or slide that should be white in color (e.g., slide background or small hole in the tissue).
    • In the Camera Settings window, click the White Balance icon then click in an area on the live image that doesn't contain any tissue. This should result in a more appropriately colored image.
  2. Adjust the Camera Settings: for best results, set the Exposure between 10 and 100 milliseconds.
    • If the image is too dim, adjust the light level on the microscope until it looks acceptable.
  3. Verify that the camera Settings are appropriate with the Camera Histogram:

    The histogram displays a graphical representation of the brightness and contrast of the current live image.

    • Adjust the camera settings until a very broad histogram is achieved .
    • The gray area should reach broadly to the edges of the graph, but should not be cut off.
    • Use the R, G, and B buttons view individual colors within the histogram.
ClosedSettings for Fluorescence
  1. The Multichannel Control panel displays the channels configured earlier.

    2. Optional : Click the Setup button to review the channel's settings.

    1. Check the Remember Video Settings box .
    2. Click the device command sequence button for the first channel; adjust the exposure in the camera settings until a full balanced histogram is achieved .
    3. Repeat step b for the other channels.
    4. Stereo Investigator has "memorized" the camera settings for each channel and is ready to apply them for acquisition.
  2. Click Acquisition>Acquire Multichannel Image.
    1. Stereo Investigator performs the acquisition tasks defined for the first channel.
    2. Stereo Investigator prompts you to adjust the camera settings. Click OK.
    3. Stereo Investigator performs the acquisition tasks for the other channels defined.
  3. Close the shutter to prevent the tissue from bleaching while setting up the acquisition.
  4. Verify that the appropriate Device Command Sequence Presets have been set up.
    • In the Multichannel Control panel, click Setup.

    • In the Acquire Setup window, click the Presets icon in the bottom left corner . You should see the name of your automated set of tasks.

Brightfield: Acquiring a background image OR Fluorescence: Acquisition settings

 

See Virtual Tissue Overview

Stereo Investigator 11 | MBF Bioscience Support Center | Downloads