Slide Loader setup 9: Select Virtual Tissue acquisition type
Select the acquisition type for the 3D Virtual Tissue image.
Single channel
Requires a command sequence, typically to set up the light path by moving mirror cubes and filters.
Why?
Running a command sequence before each acquisition enables the persistence of camera settings.
- Before acquire—Requires a command sequence even if the command sequence is "empty" (i.e., it won't lead to a change of state for any device). If there is no existing command sequence, use the Edit Command Sequences button (top right corner of the dialog) to create it.
- After acquire—Optional
Multichannel
Use the parameters already used for the 2D contour image (step 4).
Command sequence before auto-contour acquire
- Select a command sequence from the drop-down menu to maintain a light at a constant setting through the entire 3D Virtual Tissue acquisition.
Some lights require that you add a delay to the command sequence after setting the light level to give the light time to settle at the new level. Using such a command sequence at this step means that the delay only occurs once instead of occurring before each tile.
Adjust Virtual Tissue camera settings
See Slide Loader Virtual Tissue Setup Workflow