This feature has been replaced and improved!

Neurolucida 360 software, version 2020 and later, includes a new Subvolume tool that simplifies the process of dividing large and/or complex image volumes into smaller, more manageable "chunks" to facilitate tracing.

Large volume reconstruction (lvr) window

You have several options to define the subvolume in Z:

  • Set top/bottom of subvolume
  • Set top and number of images
  • Set number of images and bottom.

The distance between images is calculated automatically.

  • To view the different options, click the "cog" button.
  • To set the top or bottom of the subvolume, scroll the mouse wheel to navigate, then click the Set button.
  • To move faster through Z, use the slider under Navigation to modify the mouse wheel focus.

Mouse wheel focus: Use the slider to navigate faster or slower through Z.

 

Current location: XYZ coordinate for the center of the subvolume.

 

Desired: Modify the XYZ coordinate of the center of the subvolume.

 

The histogram displays a graphical representation of the dynamic range (visual brightness and contrast) of the image.

 Absolute black (0%) is the lower-left corner (black triangle), while absolute white (100%) is the upper-right corner (white triangle).

  • To make adjustments to how the image is displayed, drag the triangles or the square.

Optimize: Expands the range of the histogram (from black to white triangle) to its full range (255 shades of luminosity); typically provides the "best" view of the image.

Channels: If you're working with a multichannel image, select the channels that will be used to display the subvolume.

By default, all the channels are selected. Uncheck a box in the Include column to deselect the corresponding channel.

 

See also This feature has been replaced and improved!.