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Device Command Sequences

Use to define a Device Command Sequence to issue commands to a series of mechanical pieces when performing multichannel acquisitions.

The commands control the movement/state of mechanical pieces included in a motorized microscope (e.g., filter cube, shutter, filter wheel) electronically. 

 

ClosedCreating a Device Command Sequence for a motorized system

ClosedView an example of a Device Command Sequence...

 

Device Command Sequence name: DAPI Widefiled Sola

 

  1. Command 1: Microlucida turns off the fluorescent light source (Sola).
  2. Command 2: Microlucida directs the light source to the camera used for fluorescent imaging.
  3. Command 3: Microlucida only starts task 4 once task 2 is completed.
  4. Command 4: Microlucidaapplies the DAPI filter.
  5. Command 5: Microlucida only starts task 6 once task 4 is completed.
  6. Command 6: Microlucida verifies that the fluorescent shutter is open.
  7. Command 7: Microlucida only starts task 8 once task 6 is completed.
  8. Command 8: Microlucida turns on the fluorescent light source.

 

 

  1. Click Options>Device Command Sequence Setup ;Microlucida displays the Device Command Sequences dialog box .
  2. In the Add Device Command Sequence field, type the name of the sequence to be defined.
  3. Click Add. Microlucida displays the Device Command Sequence Editor dialog box .
  4. From the Device drop-down menu, select a device (the list of devices is based on the devices connected to Microlucida).
    • The same device can be used several times with different states.
  5. From the State drop-down menu, select a "state" for the device.
  6. Click Add to Sequence to add the device/state pair to the list.
  7. Repeat for all the device/state pairs necessary to complete the Device Command Sequence and click OK.

The Test State button puts a given device into the state listed in the drop-down menu in order to test the device in that state.

ClosedCreating a Device Command Sequence for a non-motorized system
  1. Create a series of messages for each microscope piece to adjust with Message Device Setup.
  2. Define a Device Command Sequence to display the messages in the order corresponding to the order of the manual adjustments.
    1. Click Options>Device Command Sequence Setup.
    2. Type a name for your sequence of messages and click Add. The Device Command Sequence Editor opens.
    3. Select "Message" from the Device drop-down menu.
    4. Select the name of the message (usually part name) from the State drop-down menu.
    5. Click Add to Sequence.
    6. Repeat steps c. through e. for each message .
    7. Click OK.
ClosedEditing a Device Command Sequence
  1. Click Options>Device Command Sequence Setup or click the Edit button in the toolbar . ClosedI can't see the Edit button...

    The Edit button may be hidden to avoid later tampering with the device command sequences you defined.

    To display the button again:

    • Click Options>General Preferences.
    • Select the Miscellaneous tab and uncheck this box: .
    • The change will take effect next time you open Microlucida.
  2. The Device Command Sequences dialog box opens.
  3. Select the name of the device command sequence that you want to edit and click Edit.
  4. Microlucida displays the Device Command Sequence Editor dialog box. 
    • To delete a device/state pair: select the device/state pair from the list and click Delete.
    • To rearrange the order of the device/state pairs: highlight a device/state pair, then select Move Up or Move Down.

 

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