Mark detected objects

Mark detected objects automatically within the entire image or within regions of interest.

This detection algorithm is based on the current image adjustments histogram, not the raw image data.

Open an image (you can't detect on a live image).
Zoom to an area containing objects representative of the objects you wish to mark.
Select a marker.
Click the Mark Detected Objects icon in the toolbar to display the Mark Detected Objects window.
Select Use Full Image or Use Region(s) of Interest under Detection Space.
Use Region(s) of Interest
- Click Use Existing ROIs to use existing contours.
  - Click a contour to select it; hold down Shift and click on contours to select multiple contours.
  - Click Done Selecting when satisfied with your selection.
- Click Draw ROIs to trace a contour.
  - Trace a contour in the Tracing window.
  - Click Finished ROIs.

While the representative object can be located outside of the region of interest, only objects within the region of interest will be marked.

Click on a representative object. Microlucida displays a preview of outlined detected objects.
- For a list of contour measurements, click the Contour Measurements icon to the right of Results. Microlucida displays the results in the Contour Measurements window.
- To use presets : See Automatic Object Detection/Presets
Adjust any of the parameters; every time you adjust a parameter, Microlucida updates the Results.
Parameters
- Sensitivity: The higher the sensitivity, the smaller the detected object around the representative sample color (displayed under Object Color ).
  - Click Apply to refresh the preview.
  - To sample a different color, click on another color in the tissue.
- Outline Point Density: Unavailable for this command.
- Exclude objects: Excludes objects from detection that are Smaller than/ Larger than the set area in microns value; use in conjunction with sensitivity.
  - Use the Quick Measure Size button for a quick estimation of the size of objects approximately circular.
- Separate Objects By Average Size: Use to increase the accuracy for the count of detected objects.
- Screen Edge Restrictions:Microlucida ignores objects that meet the criteria.
Click Mark Objects to apply the parameters.