A recent Nature Biotechnology Research Briefing, titled “Affordable centimeter-scale 3D microscopy with sub-micrometer resolution,” highlights the significance of the HySIL (hybrid solid–liquid optics) framework developed by Dr. Raju Tomer and collaborators.

The article discusses how HySIL addresses longstanding challenges in volumetric imaging by combining inexpensive long-working-distance air objectives with hybrid solid–liquid optics. This innovative approach enables scalable, submicrometer-resolution imaging of centimeter-scale samples while maintaining compatibility across a wide range of tissue-clearing methods.

Traditionally, achieving high-resolution imaging of large cleared tissues has required expensive immersion objectives with limited working distances and compatibility with only specific immersion media. HySIL overcomes these limitations by using a refractive framework with index-matched components that improves light collection, corrects aberrations, and expands imaging flexibility across diverse sample preparation methods.

The Nature Biotechnology editorial team highlighted the broader impact of this approach in the accompanying Research Briefing:

“The authors take an interesting approach to tackle the limitations of light-sheet microscopy. They pair a solid optical element with a refractive index-matched liquid to form a continuous optical system, enabling a long-working-distance air objective to achieve high resolution. The implementation makes light-sheet microscopy more scalable and less costly.”

— Editorial Team, Nature Biotechnology

As highlighted in the Research Briefing, this approach has the potential to make advanced 3D imaging more affordable, scalable, and accessible to researchers worldwide, supporting applications ranging from organ-scale biology to 3D histopathology and expansion microscopy.

The innovations described in the HySIL work have helped shape the development of the award-winning SLICE™ benchtop light sheet microscope, bringing high-performance light-sheet imaging to a broader scientific community.

We are proud to see Dr. Raju Tomer and collaborators’ innovative work continue to receive recognition from the broader scientific community and highlighted by Nature Biotechnology.

Read the full Research Briefing here: Affordable centimeter-scale 3D microscopy with submicrometer resolution

Read the original HySIL publication here: Hybrid solid−liquid optics enable scalable, high-resolution light-sheet microscopy across diverse immersion media

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Williston, VT – MBF Bioscience is honored to announce that Dr. Raju Tomer’s laboratory at Columbia University has published groundbreaking research in Nature Biotechnology, with SLICE serving as a key validation platform. The paper, titled “Hybrid Solid-Liquid Optics Enable Scalable, High-Resolution, Multi-Immersion Light-Sheet Microscopy,” introduces and validates SCOPE—an optical technology developed by Dr. Tomer’s team that enables submicron-resolution imaging using affordable, long-working-distance objectives. 

The research, led by Dr. Tomer with biologist collaborators from Columbia University, NIH/NIMH, Lehigh University, NYU Langone Health, and other institutions, showcases diverse applications including whole-brain neuronal mapping, expansion microscopy, and 3D histopathology of human cancer tissues—all performed using the SLICE platform. 

Dr. Tomer invented pLSM (projected light-sheet microscopy), which MBF Bioscience commercialized as SLICE. This collaboration demonstrates how academic innovation can be translated into accessible commercial instruments that serve the broader research community. 

“We’re grateful to support Dr. Tomer’s vision of enabling advanced microscopy in labs of all sizes,” said Jack Glaser, CEO of MBF Bioscience and co-author on the paper. “His team’s SCOPE innovation, combined with SLICE’s accessibility, is making high-resolution volumetric imaging available to laboratories worldwide.” 

The SCOPE-SLICE system demonstrated: 

• Submicron lateral resolution (<0.75 μm) using standard air objectives 

• Compatibility with diverse tissue clearing and expansion methods 

• Whole-organ imaging at cellular and subcellular resolution 

• Cost-effective 3D histopathology for research and clinical applications 

Read the full publication: https://www.nature.com/articles/s41587-026-03172-7

Learn more about SLICE: https://www.mbfbioscience.com/products/slice

The post Columbia University Researchers Publish SCOPE Technology in Nature Biotechnology; SLICE Platform Supports Validation appeared first on MBF Bioscience.

Light-sheet fluorescence microscopy (LSFM) has become an important tool for imaging large cleared tissue specimens because of its ability to rapidly acquire volumetric datasets while minimizing photobleaching and photodamage. However, many traditional light-sheet systems require complex optical engineering, expensive hardware, and specialized infrastructure that can limit accessibility for many laboratories.

The article explores how SLICE integrates projected light-sheet microscopy (pLSM), HySIL/SCOPE optics, and long-working-distance imaging configurations into a compact and scalable platform, SLICE helps reduce the complexity and cost traditionally associated with advanced light-sheet microscopy systems while maintaining the same or better research-grade imaging performance.

From large-scale brain mapping and connectomics to developmental neuroanatomy and disease-model research, SLICE enables researchers to generate high-quality whole-brain datasets with speed, flexibility, and accessibility. The system is compatible with a wide range of tissue clearing methods, including iDISCO, CLARITY, CUBIC, SHIELD, and BINAREE workflows.

We are incredibly grateful to everyone involved in the development of this technology, especially Dr. Raju Tomer and his collaborators at Columbia University.

Read the full article: Light-Sheet Microscope Design Puts Whole-Brain Imaging in Reach for Scientists

The post Making High-Performance Whole-Brain Imaging More Accessible appeared first on MBF Bioscience.

Reference

Fay, M. G., Lang, P. J., Denu, D. S., et al. (2026). ClearScope: A Fully Integrated Light-Sheet Theta Microscope for Sub-Micron-Resolution Imaging Without Lateral Size Constraints. Journal of Imaging. https://doi.org/10.3390/jimaging12030118

Background

Three-dimensional imaging of intact organs and large tissue samples is critical for studying neural connectivity and structural changes in neurological disorders. While light sheet microscopy has become a leading tool for imaging cleared tissues, conventional systems face a fundamental limitation: the orthogonal arrangement of illumination and detection optics constrains the lateral size of specimens that can be imaged — making it impossible to capture intact large rodent brains or human tissue sections in their entirety.

About ClearScope

ClearScope is based on light sheet theta microscopy (LSTM), a technology developed by Professor Raju Tomer and colleagues at Columbia University. Unlike traditional light sheet systems, LSTM uses two oblique illumination paths paired with perpendicular detection optics — enabling imaging of specimens with effectively unconstrained lateral dimensions while maintaining subcellular resolution.

Part of our broader light sheet microscopy platform – built to push the boundaries of 3D imaging

ClearScope is a technological cousin to SLICE, MBF Bioscience’s newest light sheet microscope system. Together they reflect our commitment to expanding the boundaries of 3D biological imaging — from connectomics and systems neuroscience to vascular biology, cancer biology, and organoid research.

We’re grateful to our collaborators at Columbia University and NYU Langone Health for their partnership in this work.

The post Breaking the size barrier: ClearScope light sheet microscope paper published in Journal of Imaging appeared first on MBF Bioscience.

As CEO, Glaser will focus on long-term strategy and major partnerships. As President, Dr. Pessino will oversee day-to-day operations and execution of the company’s strategic plans.

“This isn’t me stepping back, it’s us stepping up,” said Glaser. “These are challenging times in the science world, and this change ensures I can focus on positioning us for the long haul while Veronica makes sure we’re running as efficiently and effectively as possible every single day.”

Since joining as COO last summer, Dr. Pessino has driven measurable improvements across the organization, streamlining operations and bringing sharper focus to product development and go-to-market strategy. “She quickly grasped not just the nuts and bolts of our business, but its soul, what makes MBF special, what we bring to the world, what our customers need, and what our team values,” Glaser said.

The post MBF Bioscience Announces Leadership Transition: Dr. Veronica Pessino Promoted to President appeared first on MBF Bioscience.

Now available through our online store:

• Fiber Optic Cannulae

• Patch Cords

• Ceramic Split Sleeves

• Additional consumables

This new platform was created with your research needs in mind—offering a simple, streamlined experience so you can get what you need quickly and reliably.

Key Benefits:

• Order anytime
• Easy, secure checkout
• Fast, dependable delivery
• Bulk ordering options available

Can’t find what you’re looking for? Our team is here to help. Email us at info@mbfbioscience.com and we’ll assist you in locating the right tools for your setup.

At MBF Bioscience, we remain committed to supporting your research with high-quality products and responsive service.

🛒 Explore the store today and simplify your fiber photometry workflow.

The post Introducing the MBF Bioscience Web Store for our Neurophotometrics Fiber Photometry Consumables appeared first on MBF Bioscience.

MBF Bioscience Introduces SLICE™: We Reimagined Light Sheet Microscopes From the Ground Up

Breakthrough system combines high performance with unprecedented affordability and compact design, making cutting-edge light sheet microscopy accessible to labs of all sizes

WILLISTON, VT – June 9, 2025 – MBF Bioscience, a world leader in quantitative microscopy solutions, today announced the commercial launch of SLICE™, a revolutionary light sheet microscope that represents a paradigm shift in advanced imaging technology. Invented by leading researchers at Columbia University, this patent-pending innovation delivers advanced 3D imaging capabilities to research labs of all sizes while offering performance that surpasses systems costing ten times more.

Breaking Down Barriers to Advanced Imaging

SLICE addresses one of the most significant challenges in modern biological research: the limited accessibility of high-performance light sheet microscopy due to high costs and complex infrastructure requirements. This innovative system offers high sub-micron resolution imaging of biological samples, making it ideal for both individual laboratories and core facilities, effectively democratizing access to what was previously available only to well-funded institutions.

“For over three decades, MBF Bioscience has been committed to putting powerful research tools in the hands of scientists everywhere,” said Jack Glaser, President and Co-founder of MBF Bioscience. “To advance science, we had to change the math. SLICE represents the embodiment of that mission—delivering the performance researchers need at a price point that makes advanced light sheet microscopy accessible to labs that previously couldn’t afford this technology. We’re not just selling a microscope, we’re opening doors to discoveries that might never have happened otherwise.”

Dr. Raju Tomer, Associate Professor of Biological Sciences at Columbia University and inventor of the technology underlying SLICE, added: “When we developed this approach, our goal was to overcome the fundamental limitations that have kept light sheet microscopy out of reach for so many researchers. SLICE realizes that vision—it’s a system that doesn’t compromise on scientific capability while dramatically reducing the barriers to entry. I’m excited to see how the broader research community will use this technology to push the boundaries of what’s possible in biological imaging.”

Technical Innovation Meets Practical Design

SLICE comes equipped with powerful software for tera-voxel visualization, seamless image stitching, and sophisticated post-processing. The system’s compact, benchtop-ready design enables placement in diverse research environments, including higher biosafety level facilities, small lab spaces, and even inside incubators where experiments are conducted.

Key features of SLICE include:

• Exceptional Performance-to-Cost Ratio: Performance surpassing systems costing ten times as much
• Multi-wavelength Compatibility: 3 illumination wavelengths for popular labels such as: GFP, Thy1-GFP, eYFP, Alexa 514, 633, 647, mCherry, tdTomato
• Versatile Clearing Compatibility: Compatible with iDISCO, CLARITY, SHANEL, CUBIC, BINAREE and virtually all other optical clearing techniques
• Comprehensive Software: BrightSLICE software for acquisition, visualization, and post-processing
• Seamless Integration: Seamlessly integrates with MBF Bioscience’s suite of analytical tools, including Neurolucida 360, NeuroInfo and NeuroDeblur

Expanding Research Capabilities

The SLICE system enables researchers to image labeled cells and neuronal processes throughout cleared tissue with exceptional clarity and speed. A wide range of proteins and dyes can be used with SLICE, including c-Fos, tdTomato, mCherry, podocalyxin, CD31, Acta2, mScarlet, and Thy1-eYFP, making it suitable for diverse research applications across neuroscience, developmental biology, cancer research, and beyond.

Advanced image stitching produces 3D image volumes suitable for MBF Bioscience’s AI-based quantitative analysis software to produce accurate data for analyzing morphometry, cell populations, sub-cellular processes, vasculature and fluorescence properties, enabling researchers to conduct sophisticated analyses previously limited to specialized facilities.

About MBF Bioscience

MBF Bioscience is a biotech company that develops microscopy software and hardware for bioscience research and education. MBF Bioscience’s primary location is Williston, Vermont, United States and has offices that market, sell, and support its line of hardware and software products in Ashburn, Virginia; San Diego, California; Europe, and Asia. The company was founded in 1988 as MicroBrightField, Inc. by the father and son team of Dr. Edmund Glaser and Jack Glaser, and has been instrumental in advancing quantitative microscopy for over three decades.

MBF has received numerous awards for innovation, customer service, and employee satisfaction, including the US Small Business Administration’s Tibbetts Award for excellence in high technology, the Vermont Small Business Person of the Year award, and the Best Places to Work in Vermont award. MBF’s tools have been cited in over 16,000 peer-reviewed journal publications, underscoring the company’s impact on global scientific research.

Availability and Pricing

SLICE is now available for order, with 30-day lead time for delivery. The complete system, including the light sheet microscope, BrightSLICE software, and computer workstation, is priced under $75,000 – representing exceptional value for advanced 3D imaging capabilities. For demonstrations, technical specifications, or ordering information, visit www.mbfbioscience.com/products/slice or contact MBF Bioscience directly.

Media Contact: Pasang Sherpa, Marketing Manager

Phone: +1 (802) 288-9290

Email: pasang@mbfbioscience.com

The post MBF Bioscience Introduces SLICE™: We Reimagined Light Sheet Microscopes From the Ground Up appeared first on MBF Bioscience.

This Year’s Winning Pieces

• 1st Place: Totally Tubular by Joey Tidei, Loyola University Chicago
• 2nd Place: Neurosphere by Adam Soos, Semmelweis University
• 3rd Place: Unwelcome Guest: SARS-CoV-2 Arriving in the Brain by Danielle Beckman, UC Davis Center for Neuroscience

Each of these stunning pieces showcases the complexity and beauty of neuroscience through artistic expression. Whether exploring cellular structures, neural networks, or the interaction of pathogens with the brain, these works offer a fascinating glimpse into the world of neuroscience.

Inspired by these works? Our monthly NeuroArt contest is the perfect opportunity to showcase your creativity and passion for neuroscience. Whether you’re an artist, scientist, or someone who appreciates the beauty of the brain, we welcome your submissions.

Thank you for being part of the NeuroArt community—we can’t wait to see what you create next!

The post Celebrating the NeuroArt Image Contest Annual Grand Prize Winners appeared first on MBF Bioscience.

The ongoing freeze and efforts to slash NIH funding pose an existential threat to the future of biomedical research. As scientists, we see firsthand how federal investment in research leads to medical breakthroughs that benefit all people—curing diseases, improving quality of life, and strengthening our nation’s position as a leader in innovation. Yet, despite the undeniable impact of biomedical research, funding is being slashed, and attempts to stall critical programs are underway. If these cuts continue, the consequences will be felt for decades.

Scientific progress does not happen overnight. It is the result of sustained funding, collaboration, and commitment. Drastic budget reductions will stall projects, disrupt labs, and drive talented researchers—especially early-career scientists—out of the field. The ripple effect will be devastating, slowing the development of new treatments, vaccines, and technologies that millions rely on.

Particularly alarming is the targeting of programs designed to support historically excluded groups, including people of color, women, and LGBTQ scientists. Cutting these initiatives is not only unjust but short-sighted. Diversity in science drives innovation. The strongest scientific breakthroughs arise from collaboration among people with different perspectives, experiences, and expertise. If we push talented minds out of research, we all lose.

What are we doing about it? We are mobilizing. We are working with academic institutions and industry partners to educate the public on the necessity of NIH funding. We are calling on our representatives and senators to demand support for biomedical research. We encourage all scientists, students, and allies to join us in this fight—write, call, and speak up. Science cannot be a partisan issue when human lives are at stake. We urge policymakers to recognize what is at risk and take action to protect the future of biomedical research. The cost of inaction is simply too high.

For further discussion on the impact of NIH funding changes, listen to Vermont Edition, where Jack Glaser, CEO/Founder, shares insights on how these shifts affect research. Listen here.

We want to hear from you. What challenges are you facing? How can we help? Reach out—let’s navigate this together.

Sincerely,
MBF Bioscience 

The post Protecting the Future of Biomedical Research: A Call to Defend NIH Funding appeared first on MBF Bioscience.

Our Open Office Hours are designed to be interactive and flexible, allowing attendees to explore specific product features, discuss applications, and gain insights into best practices for using our technology effectively. These sessions are not only an opportunity to address immediate concerns but also to learn about advancements in our software and hardware solutions that could enhance your research efficiency. Additionally, they provide a space to connect with fellow scientists and professionals, fostering a collaborative environment where ideas and experiences can be shared.

Sessions are held every Tuesday at 12 PM ET and are conducted virtually, making them easily accessible to researchers worldwide. There is no need for formal scheduling—simply join the session at your convenience to engage with our team and other attendees. Whether you have specific technical questions, need troubleshooting assistance, or simply want to explore new ways to integrate MBF Bioscience technology into your work, we welcome you to participate.

We look forward to connecting with you and supporting your research efforts.

📅 Every Tuesday at 12 PM ET |  📋 Register here

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