Vesalius

VesaliusTM 

The Ultra-fast, Multi-channel Imaging System for Confocal Whole Slide Scanning and Intact Cleared Tissue Imaging

Vesalius is a versatile resonant scanning laser confocal system for fast 2D and 3D imaging of slide mounted specimens and cleared tissue imaging. Designed in collaboration with our partner Caliber ID, Vesalius utilizes a strip mosaic scanning process to rapidly and accurately collect fluorescent image data while the stage moves the specimen continuously in the y-axis. This scanning method accelerates image acquisition speed while eliminating the need for stitching field of view (FOV) tiles along the vertical axis.  Using innovative technology to scan in up to 5 fluorescence wavelengths, Vesalius is designed to fulfill two advanced imaging functions needed in research laboratories and core facilities.

  • Confocal Whole Slide Scanning
  • Imaging of Intact Cleared Specimens

 

Ultra-fast confocal fluorescence whole slide scanning

Vesalius provides unparalleled high-throughput, multi-channel fluorescence confocal whole slide imaging. With its high-speed resonant confocal scanner and precise linear motor stage, Vesalius substantially reduces the time needed for image acquisitions compared to conventional confocal imaging systems with no compromise in image quality. Vesalius is the perfect solution for imaging multiple slides quickly. 

Highlights

  • Vesalius collects images at a substantially faster rate than other confocal technologies, reducing the total scan time of a specimen by up to a factor of 10.
  • Four laser channels for single sequential, or simultaneous (up to 2 channels) acquisition of fluorescent labels and an infrared channel. Operating wavelengths of 405, 488, 561, 640, and 785nm. 
  • Can be configured to work with a large variety of air, oil, and water immersion objective lenses up to 100x magnification
  • Flexible slide size handling with custom stage inserts to accommodate 1x3”, 2x3”, and slides up to 80x120mm in size.
  • Up to 4 1x3” slides can be accommodated on a single stage insert
  • High resolution 2D and 3D imaging suitable for quantitative analysis with MBF image analysis software
  • Efficient resonant laser scanning technology minimizes photo-damage to specimens while optimizing image quality and detail

High resolution imaging of intact cleared tissue specimens

Vesalius is as versatile as it is fast. It is the result of numerous interdisciplinary collaborations and many hours of research and development. In addition to its amazing confocal slide scanning performance, it can also be used for imaging cleared tissue specimens. 

  • Custom specimen chambers for imaging larger specimens, including whole mouse brains or tissue slabs up to 6mm thick
  • CLARITY, iDISCO, uDISCO, CUBIC, SeeDB, Sca/e and more
  • Objective lenses are available in magnifications including: 10x, 16x, 20x, and 25x 
  • Fully automated stitching algorithms efficiently create high resolution mosaics of thick and thin tissues
  • An additional 4x optical zoom within the microscope head allows for better finer imaging of small structures

 

Powerful reconstruction and data analysis tools

  • Images acquired with Vesalius can perform complex image analyses such as cell counting, neuron tracing, and stereological analysis. A number of powerful analysis tools are included in the Vesalius system:
  • Automated cell detection and rapid stereological analysis in 2D and 3D images (Cellairus)
  • Creating full-resolution 3D images from 2D serial section image data (BrainMaker or TissueMaker)
  • Automated neuron tracing and quantitative analysis (Neurolucida 360)
  • Automated vessel tracing and quantitative analysis (Vesselucida 360)
  • Stereological analysis (Stereo Investigator – Whole Slide Edition, and Stereo Investigator – Cleared Tissue Edition) 

 

 

What research applications are laboratories using Vesalius for?

Vesalius was developed in response to the clear needs of the neuroscience research and community which include high-throughput, multi-channel fluorescent, high-resolution confocal imaging of entire brain sections, the need for appropriate data sharing technologies, and requirements for reproducible and reliable quantitative measures of biological features.

  • 3D reconstruction of brain sections for neuron tracing, vessel tracing, and connectomics
  • High resolution imaging of cell proliferation and embryo development
  • Rapid analysis of fluorescently labeled specimens in gene and protein mapping, cytoarchitectural analysis, and pathology
  • Stereologic cell counting coupled with reliable automated cell detection
  • Vesalius has been instrumental in conducting research in many fields including neuroscience, pulmonology, nephrology, cardiology, and endocrinology.
  • Image data is saved in the HDF5 format to optimize efficiency and make it easy for users to manage and transfer their image data whether it’s small or in the multi-TB range

 

Vesalius can help you achieve your research goals

  • Ultra-fast imaging results in improved efficiency of data collection leading to more data, higher confidence in statistical analysis, and the ability to conduct more studies
  • Multi-purpose imaging solution saves money and provides more capabilities in less space
  • Increase productivity of lab personnel
  • Complete research projects faster, publish more papers, and open the door to more collaborations
  • Quickly complete pilot studies and collect preliminary data for grant applications

 

Vesalius Technical Specifications

Resolution
  • Horizontal Optical Resolution = 0.5 µm
  • Vertical Optical Resolution = 0.76 µm
  • Pixel resolution per image = 1024 x 1024 pixels

Field

  • Single Frame Field of View = 400 x 400 µm standard objective
  • Mapped Field 120 x 80 mm

 

 

 

Magnification

  • 10x air, 20x air/water/oil, 40x water/oil, 60x water/oil, 100x water/oil objectives
  • Size dimensions = Length 18.25" x Width 15.5" x Height 16"
  • Weight = 58lbs

Laser power

  • 405nm = 100mW
  • 488nm = 100mW
  • 561nm = 100mW
  • 640nm = 70mW
  • 785nm = 90mW

 

Professional Technical Support

When you call us you will speak with a person - not an automated system. Talk to us about your hardware, software, or experimental design questions. Our team includes Ph.D. neuroscientists and experts in microscopy, stereology, neuron tracing, and image processing; ready to help you over the phone or online.

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