Vesalius

Vesalius 

The Ultra-fast, Multi-channel Imaging System for Confocal Whole Slide Scanning and Intact Cleared Tissue Imaging

Vesalius is a powerful and versatile image scanning device available from MBF Bioscience, designed in collaboration with our partner Caliber ID.  Using innovative technology to scan in up to 5 fluorescence wavelengths, Vesalius is designed to fulfill two advanced imaging functions needed in research laboratories and core facilities.

  • Confocal Whole Slide Scanning
  • Imaging of Intact Cleared Specimens

Vesalius features a new strip mosaic image capture process coupled with its proprietary image-stitching algorithm to create cellular-level images with unprecedented down-to-the-pixel accuracy and perfect homogeneity.

Vesalius is available in configurations that enable it to perform either 2D or 3D whole slide scanning or 3D imaging intact cleared specimens, or both.

 

Ultra-fast confocal whole slide scanning

Vesalius provides unparalleled high-throughput, multi-channel fluorescence confocal whole slide imaging. The combination of fast scanning speed and image quality are not available in any other imaging instrument.  With its high-speed resonance confocal scanner and scanning stage, Vesalius substantially reduces the time needed for image acquisition compared to conventional confocal imaging systems — with no compromise in quality —   and will substantially reduce the acquisition time of your next imaging project. If you need to image a lot of specimens, Vesalius is the perfect solution.

Highlights

  • Vesalius collects images at a substantially faster rate than other confocal technologies, reducing the total scan time of a specimen by up to a factor of 10.
  • Five laser lines for single, sequential, or simultaneous (up to 2 channels) acquisition of fluorescent labels. Operating wavelengths of 405, 488, 638, 561, and 785nm. 
  • Can be configured to work with a large variety of dry, oil, and water immersion objective lenses up to 100x magnification
  • Flexible slide size handling with custom slide holders for non-standard sizes or multiple slides
  • High resolution 2D and 3D imaging suitable for quantitative analysis with MBF image analysis software
  • Efficient resonant laser scanning technology minimizes photo-damage to specimens while optimizing image quality and detail

High resolution imaging of intact cleared tissue specimens

Vesalius is as versatile as it is fast. In addition to its amazing confocal slide scanning performance, it can also be used for imaging cleared tissue specimens. 

  • Custom specimen chambers for imaging larger specimens, including whole mouse brains or tissue slabs up to 6mm thick
  • CLARITY, iDISCO, uDISCO, CUBIC, SeeDB, Sca/e and more
  • Objective lenses are available in magnifications including: 10x, 16x, 20x, and 25x 
  • Fully automated stitching algorithms efficiently create high resolution mosaics of thick and thin tissues
  • An additional 4x optical zoom within the microscope head allows for better finer imaging of small structures

 

Powerful reconstruction and data analysis tools

  • Images acquired with Vesalius can perform complex image analyses such as cell counting, neuron tracing, and stereological analysis. A number of powerful analysis tools are included in the Vesalius system:
  • Automated cell detection and rapid stereological analysis in 2D and 3D images (Cellairus)
  • Creating full-resolution 3D images from 2D serial section image data (BrainMaker or TissueMaker)
  • Automated neuron tracing and quantitative analysis (Neurolucida 360)
  • Automated vessel tracing and quantitative analysis (Vesselucida 360)
  • Stereological analysis (Stereo Investigator – Whole Slide Edition, and Stereo Investigator – Cleared Tissue Edition) 

 

 

What research applications are laboratories using Vesalius for?

Vesalius was developed in response to the clear needs of the neuroscience research and community which include high-throughput, multi-channel fluorescent, high-resolution confocal imaging of entire brain sections, the need for appropriate data sharing technologies, and requirements for reproducible and reliable quantitative measures of biological features.

  • 3D reconstruction of brain sections for neuron tracing, vessel tracing, and connectomics
  • High resolution imaging of cell proliferation and embryo development
  • Rapid analysis of fluorescently labeled specimens in gene and protein mapping, cytoarchitectural analysis, and pathology
  • Stereologic cell counting coupled with reliable automated cell detection
  • Vesalius has been instrumental in conducting research in many fields including neuroscience, pulmonology, nephrology, cardiology, and endocrinology.
  • Image data is saved in the HDF5 format to optimize efficiency and make it easy for users to manage and transfer their image data whether it’s small or in the multi-TB range

 

Vesalius can help you achieve your research goals

  • Ultra-fast imaging results in improved efficiency of data collection leading to more data, higher confidence in statistical analysis, and the ability to conduct more studies
  • Multi-purpose imaging solution saves money and provides more capabilities in less space
  • Increase productivity of lab personnel
  • Complete research projects faster, publish more papers, and open the door to more collaborations
  • Quickly complete pilot studies and collect preliminary data for grant applications

 

Vesalius Technical Specifications

Resolution
  • Horizontal Optical Resolution = 0.5 µm
  • Vertical Optical Resolution = 0.76 µm
  • Pixel resolution per image = 1024 x 1024 pixels

Field

  • Single Frame Field of View = 400 x 400 µm standard objective
  • Mapped Field 120 x 80 mm

 

 

 

Magnification

  • 10x air, 20x air/water/oil, 40x water/oil, 60x water/oil, 100x water/oil objectives
  • Size dimensions = Length 18.25" x Width 15.5" x Height 16"
  • Weight = 58lbs

Laser power

  • 405nm = 100mW
  • 488nm = 100mW
  • 561nm = 100mW
  • 640nm = 70mW
  • 785nm = 90mW

 

Professional Technical Support

When you call us you will speak with a person - not an automated system. Talk to us about your hardware, software, or experimental design questions. Our team includes Ph.D. neuroscientists and experts in microscopy, stereology, neuron tracing, and image processing; ready to help you over the phone or online.

Still Have Questions? Just Ask!