Set up the subject
Subject Information
Type your name, the subject name, and any other information that you think will be helpful to someone who may work with this subject in the future.
Sampling Parameters
Choose whether or not to use a previously saved sampling configuration. If yes, click on the browse (…) icon. In the Sampling Parameter Chooser window, highlight the parameter set you wish to use and click OK.
Enter Serial Section Information
- # of sections to count: Number of actual sections you will be counting for this subject across all of the slides.
- Section’s cut thickness: Tissue cut thickness or block advance.
- Section evaluation interval: Interval of the serially cut sections that you are going to count.
- To count every other section, enter 2.
- To count every third section, enter 3, etc.
- Starting section number: Number of the first section you start from in your serial sections
- The first sampling section should be randomly selected.
- If you don't want to keep track of the section count from the microtome, leave this number as "1."
- Z-value of first section: This number is automatically adjusted based on the Starting Section Number entered before; it reflects an interval of the cut thickness.
Example
| Section’s cut thickness | Starting section number | Z value of first section |
| 50µm | 1 | 0 |
| 50µm | 2 | 50 |
| 50µm | 3 | 100 |