Vertical Spatial Grid
Use Vertical Spatial Grid to estimate the volume and surface area of objects contained within a vertical uniform random stack of parallel sections, such as a confocal image stack.
Within each section, an array of parallel cycloid segments and grid points are used to estimate the surface area.
Procedure
- Click Probes>All probes>Surface>Vertical Spatial Grid.
- Adjust the value and click OK.
- Right-click and select Define Vertical Axis.
- Click all of the points where the surface of the object of interest intersects the cycloid array.
- To move to the next focal plane, right-click and select Move Up One Plane or Move Down One Plane.
- To mark intersections within the region of interest (for estimating area), right-click and select Mark Grid Points. An array of points is displayed.
Mark all grid points that fall within the profiles of objects or areas of interest.
- Once all points are marked within the particles of interest, click Probes>All probes>Surface>Vertical Spatial Grid again to exit the probe.
The results are immediately displayed in the Sampling Results window.