Proximity

Use to analyze the distance between two trees (e.g., distance between a neuronal fiber and a blood vessel).

Neurolucida Explorer compares the trees and determines the percentage of the fiber in closest proximity to the neighboring fiber.

Procedure

Select both trees in the Data List window by clicking their labels.
Click Analyze>Neuron data>Anatomical and open the Proximity Analysis window opens .
- Granularity for Tree Decomposition: Distance between points on each individual fiber.
Ex: Setting the granularity at 2 for a 10-µm fiber results in 5 points on the fiber.
- Bins for Diameter: Available if you selected Diameter Details.

Tree: Neurolucida Explorerassigns number 1 and number 2 to the trees of interest.

Bin Start and Bin End: Shortest distance bin between the points.

Length: Length of the tree within the range of the shortest distances.

Percent Length: Percentage of the tree length that is within the distances specified in Bin Start and Bin End.

Order: If you selected Branch Order, values are broken down by order.

Bearden, S.E., and Segal, S.S. (2005). Neurovascular alignment in adult mouse skeletal muscles. Microcirculation. Mar 12(2):161-1677.