"Stereo Investigator is the most reliable tool for collecting unbiased stereology data. It’s backed by excellent technical and research support teams at MBF. You can call to ask about stereology probes, tissue preparation, microscope hardware, basically anything regarding your stereology study."
Mark West, M.D., Ph.D.
Aarhus University, Denmark
"Stereo Investigator Cleared Tissue edition is great! We used it on large light-sheet datasets obtained from human brains with 4-channel imaging for different markers of neuronal populations. We could obtain cell type-specific stereologic data from a cytoarchitecturally defined region of the neocortex. We then registered the entire stereologic dataset, including laminar boundaries and sampled cells, to a high-resolution MRI dataset."
Patrick R. Hof, Ph.D.
Icahn School of Medicine at Mount Sinai
"MBF Bioscience’s software helped us immensely to differentiate and follow the changes caused by chronic microglia activation in various areas of the brain during aging, but also to quantify the effects of different modified curcumin products, which otherwise would have been impossible."
Dr. Erika Gyengesi
Western Sydney University, Australia
"The MBF team led by Dr. Tappan has been highly receptive to the project needs and constantly strived to improve TissueMapper to enable smooth handling of large image stacks, in-depth annotation, and seamless mapping of external data sets onto a 3D map of the intrinsic cardiac nervous system. The all-around expertise of scientists and technologists at MBF was crucial to preprocess and assemble the large scale imaging data to jumpstart the 3D map building process. It is heartening to see MBF, a commercial entity, drive the development, standardization and dissemination of open data formats for complex anatomical annotation towards enabling friction-free exchange of these data sets in the scientific community."
Rajanikanth Vadigepalli, Ph.D.
Thomas Jefferson University
"Biolucida is an amazing platform for using virtual histology in medical education that I have been using to aid my teaching practices for years. The medical students have also shown great interest in your virtual slides and that has helped them achieve better results - findings that I have also published."
Marko Kostovski, MD, PhD
Institute of Microbiology and Parasitology Faculty of Medicine-Skopje
University Ss Cyril and Methodius in Skopje
"I am so happy to be a customer of your company. I always get great help related with your product or not. With the experienced members, you are the best team I've ever met. All of your staff are very kind and helpful. Thank you for your great help and support all the time."
Marmara Üniversitesi Tıp Fakültesi, Turkey
"It was so helpful to have Biolucida already in place with the pandemic and move to online instruction. Everything worked so smoothly!"
Dr. Sarah Werning, Associate Professor
Des Moines University
"Vesselucida enables us to visualize and quantify microvascular networks in a way that has not been done before. The ability to render 3D maps is integral to analysis of network structure, remodeling and blood flow distribution."
Dr. Nicole Jacobsen
University of Missouri
"WormLab has taken our behavior studies to another level, since we can test more complex hypotheses to detect slight changes in the behavior of worms. After two years of our lab team working regularly with WormLab I can say with full confidence that WormLab provides far superior data than the other conventional programs used for worms. Our work with WormLab has been extremely rewarding because it puts us on the frontier of the behavior studies of C.elegans and allows us to carry out differentiated experiments, with software that is easy to use and needs only simple equipment."
Dr. Félix Soares
University Professor and Researcher, Universidade Federal de Santa Maria, Brazil
Former Visiting Scientist at Albert Einstein College of Medicine, USA
"MBF Bioscience is extremely responsive to the needs of scientists and is genuinely interested in helping all of us in science do the best job we can."
Sigrid Veasey, M.D.
Professor of Medicine
University of Pennsylvania
"After examining different vessel quantification tools for use with neovessel formation in the heart, we chose to use Vesselucida 360 because it offered us flexibility in viewing and adjusting vessel geometries in 3D to accurately represent our microCT dataset. Also, the quantitative parameter extrapolation is excellent."
Dr. Kareen Coulombe
"We really appreciate the help from all the staff at MBF Bioscience. Expert support combined with their excellent product WormLab are essential for our research on C. elegans behavior in my lab."
Jihong Bai, Ph.D.
Basic Sciences Division, Fred Hutch
"We’ve been very happy for many years with MBF products and the course of upgrades and improvements. Your service department is outstanding. I have gotten great help from Geoff and many others with the software and hardware."
William E. Armstrong, Ph.D.
Director of Neuroscience Institute
University of Tennessee
"Dan was very helpful and detailed. He heard my experimental design in detail and suggested what would be the appropriate way to scan and then analyze the data. My overall experience was great, the support from MBF is prompt and it seems that they are eager to help with individual problems and questions."
Rashi Priya, Ph.D.
University of California San Francisco
"Neurolucida 360 is a remarkable system that has provided us with tools to study dendritic architecture in cultured neurons, rodent models, and humans, with outstanding precision and detail."
Jeremy Herskowitz, Ph.D.
University of Alabama at Birmingham School of Medicine
"Using Neurolucida, we were able to collect, store, and analyze large amounts of data with more precision and accuracy than would have been possible without the digital interface. The software allowed us to trace dendrites, get precise thickness measurements, and categorize spine types easily during tracing. The built-in Sholl analysis and spine analysis tools resulted in quick quantification of all the measurements that would have taken hours to achieve without Neurolucida."
A graduate student working in Dr. Aoki's lab, New York University.
"MBF Bioscience was highly responsive and innovative in helping me achieve my research goals. I wanted to analyze extremely large virtual histology images in Neurolucida that were originally created using the Aperio and Hamamatsu Nanozoomer image file formats. MBF initiated the dialog with each company and was able to add the support. They further modified Neurolucida to meet the needs of the unique problems and benefits associated with virtual images. Great company to work with."
Harvey J. Karten, M.D.
Professor of Neurosciences and Psychiatry at UCSD School of Medicine
"The support team at MBF Bioscience has been great to work with. As a beta test site for Vesselucida, we are in constant communication. Their staff is professional and responsive to the problems we have identified and our requests for specific applications. Their technical support enables adaptation of this software to specific requirements of those studying the microcirculation."
Rebecca Shaw, Research Specialist.
University of Missouri
"Neurolucida 360 is very useful for doing quantitative analysis of neuronal morphology. Multiple parameters extracted by using this quantitative analysis program represent the intrinsic features of neurons. For example, axonal segment lengths, maximum branch angles and bouton density usually stay consistent for a neuron type although the general axonal and dendritic clusters may vary significantly according to the locations of the neuron type. When combined with multiple-neuron patch clamp recording, the quantitative morphological analysis has served as an essential step to enable us to successfully carry out the following research. We have been able to reveal the organizing principles of inhibitory networks (Gupta et al., 2000); define and systematically study a new subclass of basket cell – nest basket cell (Wang et al., 2002); systematically study Martinotti cells in anatomy, electrophysiology and synaptology (Wang et al., 2004); discover glutamatergic differential innervation of different target neurons by single PCs (Markram et al., 1998) and anatomical principles for differential glutamatergic innervation based on a large number of reconstructed connections (Wang et al., 1999). In addition, we have been able to reveal cellular, synaptic and network features of the layer 5 pyramidal microcircuitry (Wang et al., 2006). Furthermore, a unique reconstruction data base of over ten thousand neurons and hundreds of synaptic connections has been developed, which will be essential to put the neuronal microcircuitry together with the biologically realistic computer simulations in the Blue Brain Project."
Yun Wang, Ph.D.
Allen Institute for Brain Science
"We have used Neurolucida in many of our research projects. Most recently, we used this software to reconstruct and analyze the structure of pyramidal neurons in the primary visual cortex versus the dorsolateral prefrontal cortex of rhesus monkeys, as described in Amatrudo, Weaver et al. 2012. Our ongoing studies continue to benefit enormously from the AutoNeuron, AutoSpine and AutoSynapse modules of Neurolucida, developed by MBF."
Jennifer Luebke, Ph.D.
"I have called several times for many different issues and the customer service and support team here is the most helpful and most pleasant to work with. I have never had a better experience calling in for customer service related issues."
Benjamin Babbitt, Undergraduate Student
University of Connecticut
"I'm a huge fan Neurolucida's cell tracing and auto-detection features, which greatly assisted me in characterizing the morphology of abnormal neurons. Neurolucida is easily learned, intuitive, and was clearly designed by scientists."
Michael Hester, Graduate Student
University of Cincinnati
"Stereo Investigator is powerful yet easy to use. The staff at MBF Bioscience is always helpful answering our questions and recommending optimal ways to conduct our stereological analyses."
Matthew Goldberg, Ph.D.
University of Texas Southwestern Medical Center
"Since we purchased our system four years ago, our experience with the MBF equipment and especially the MBF people has been outstanding. I cannot speak any higher about their professionalism and attention for our needs. "
Bogdan A. Stoica, M.D.
University of Maryland, School of Medicine
"Stereo Investigator was particularly useful for estimating cell density in larger brain structures, such as the inferior colliculus."
Matt Pitts, Ph.D.
University of Hawaii
"Stereo Investigator is great for Stereology. Although there is no field representation in the UK, the team in Vermont have been excellent at supporting us in our research applications. With online support and real time meetings and webinars, we've been able to make substantial progress on our research projects. MBF aren't afraid of a technical challenge, which is excellent, and have managed to provide us an excellent solution for our complex research needs."
Anne Wheeler, Advanced Imaging Facility Manager
Queen Mary, University of London (UK)
"We have been tracing dendrites for almost 30 years, using a variety of home-grown and commercial systems. We have used Neurolucida for almost 15 of those years now, and it is by far the most powerful, reliable, flexible, and easy to learn and use system of all that we’ve worked with. Neurolucida is clearly the product of people who thoroughly understand and appreciate quantitative neuroanatomy. This system has been absolutely indispensable to our research, resulting in over 25 publications. I can’t imagine our science without Neurolucida! Furthermore, the support provided by MBF has been simply outstanding, including program modifications, hardware support and development, troubleshooting, you name it - and always provided quickly, cheerfully, and knowledgeably. Nobody does it better!"
Dale Sengelaub, Ph.D.
"Neurolucida 360 is clearly the best in the field - reliable, accurate, and very importantly, easy and intuitive to use. I would not try any other system."
Jeffrey Kordower, Ph.D.
Rush Presbyterian Medical Center
"Stereo Investigator has been the workhorse of our Microvascular Pathology Lab for the last six years. We have been able to measure vascular density with an accuracy never before available to our studies and more recently SI has provided efficient and precise comparisons of hypoxia and ischemic damage in cerebral vessels and neurons. The helpfulness, availability and experience of the MBF staff has been extraordinary. I would recommend any of their products wholeheartedly."
Clara Thore, Ph.D.
Wake Forest University
"Neurolucida 360 is the future of quantitative neuromorphology. I am particularly excited about Neurolucida 360's interactive function because it will allow for much more efficient tracing of Golgi stained neurons, which are notoriously difficult to quantify. Cajal would be proud!"
Bob Jacobs, Ph.D.
"I have been using the Neurolucida and Stereo Investigator software from MicroBrightField for the last 5 years, and this software has become an essential part of my research program. Whether it is 3 dimensional reconstruction, counting particular particles or mapping the developing and adult vascular, these programs are a scientific must. I personally have been able to push my research in ways that would otherwise not have been possible. Furthermore, the ability to quantitate and detect subtle differences is and will continue to be critical to my scientific program. I would highly recommend these companion programs to any research scientist."
Sunder Sims-Lucas, Ph.D.
Columbus Children’s Research Institute
"We have used Stereo Investigator for over 10 years now. The service and technical support is always great and they keep adding new updates with more tools."
Paula Bickford, Ph.D.
University of South Florida
"MicroBrightField has THE BEST customer service in the industry. The staff at MBF go above and beyond basic customer service, time and time again. They are always available to provide efficient, knowledgeable, and unmatched support to both seasoned and novice users of Neurolucida and Stereology."
Meaghan Wilkin, Ph.D. Candidate
Queen's University, Ontario, Canada
"Stereo Investigator is an excellent research tool for unbiased stereology. In our research group when we set out to quantify the complex network nerves residing in the stomach mucosa. With Stereo Investigator we were able to get accurate estimates of numbers and show differences between your study groups without the introduction of sampling bias."
Mona Selim, Research Associate
University of Minnesota
"I am amazed at the speed and accuracy of Neurolucida 360. The automatic tracing program is intuitive and easy to use."
Gwen Wendelschafer-Crabb, Ph.D.
University of Minnesota
"I rarely have encountered a company so committed to support and troubleshooting as MBF."
Andrew Hardaway, Graduate Student
"I have been using Neurolucida for over 20 years now. It sets the standard for what a tracing program should be able to do, and the ease of use has continually increased over the years. I have undergraduates using the program within a week. Moreover, the support MBF Bioscience provides is unmatched--they are the best."
Bob Jacobs, Ph.D.
"I have also been using Neurolucida for over 20 years with over 50 publications that have relied at least in part on the software. The product is marvelous and the support is the best of any software product I own. The work we do would be next to impossible without Neurolucida. The software is so intuitive that even high school students can do basic tracing almost immediately."
Ruth Stornetta, Ph.D.
University of Virginia
“Your company is one of the greatest for customer service. I always feel like a priority with your representatives.”
Sally Krajewski, Research Specialist
University of Arizona
"The quality and rigor of the work produced by MBF Labs is impressive, and we appreciate the contributions of the scientific staff in the design of the investigative work and the analysis of the data. CHDI values their professional, proactive approach, which has made this collaboration an integral part of our effort to find a treatment that slows or delays Huntington's disease."
David S. Howland, Ph.D.
"Our research group has been using Neurolucida to trace epidermal nerve fibers in skin biopsies for at least 15 years. The software is not only great at tracing nerve fibers for counts, length and branch points but as it keeps track of nerve coordinates you can learn about distribution; extremely valuable data especially very early in disease states where nerve fiber counts may appear normal but the distribution of fibers is usually not normal."
Mona Selim, Research Associate
University of Minnesota
"Our system worked almost every day for the last eight years with no major failure (we published more than 15 papers using Neurolucida). A terrific machine!"
Ferdinando Rossi, M.D., Ph.D.
Rita Levi Montalcini Center for Brain Repair, University of Turin
"Our Stereo Investigator system works perfectly. We use it every day and don't have any problems whatsoever. Thanks for the good system."
Grazyna Rajkowska, Ph.D.
The University of Mississippi Medical Center
"I was able to create excellent 3D maps using the Solid Modeling module of Neurolucida. Without your expert advice, I'm not sure our poster would have been ready for presentation."
Susan Lantz, Biologist
National Center for Toxicological Research
"The AutoNeuron module is very impressive in its design to 'follow' nerve fibers; we have been using it for almost 8 years. We use it to successfully trace complex nerve networks, for example those found around sweat glands in the skin."
Mona Selim, Research Associate
University of Minnesota
"I wanted a Lucivid from the first moment I looked through a microscope with one attached. Nothing beats looking through a microscope to see the true beauty, intricacies and details of histological sections, and the ability to simultaneously collect data with the superimposed computer display is phenomenally powerful and satisfying. We’ve collected data using a Lucivid for almost 15 years, and I can't imagine collecting our data without one. The new generation Lucivid continues a venerable tradition, and the incredible linearity, brightness and clarity of the display makes it a wonderful (and in our case, critical) complement to the Neurolucida system."
Dale Sengelaub, Ph.D.
"We find that using the Lucivid is excellent for making highly accurate digital reconstructions of neurons which are difficult to see with a digital camera connected to our microscope. For instance, more dendritic spines and axonal boutons are visible in the binocular eyepieces than on the screen showing the camera image. The use of Lucivid makes easier to record these details with accuracy and to use them in detailed fine structural analysis."
Rudolf Kraftsik, Senior Scientist
University of Lausanne, Switzerland
"We are enjoying using your fantastic software [Neurolucida] and it is making a difference to our work."
Andrew Randall, Ph.D.
Professor in Neurophysiology
University of Exeter Medical School
"Very strong company we have worked with for over a decade. Always very professional and service/customer oriented."
Kevin Brewer, Technical Services Manager
Wake Forest University School of Medicine, North Carolina
"Neurolucida is one of the very few systems that combines complex morphometrical quantification with beautiful display of the results."
Ahmed Salehi, M.D., Ph.D.
Clinical Professor, Department of Psychiatry and Behavioral Sciences
Stanford Medical School
"Stereo Investigator is a excellent stereology system. It is easy to work with and the workflows help you to work very efficiently. It is a crucial research tool for obtaining estimates of number of neurons, length of capillaires, length density of fibers, volume measurements of regions of interests or neurons, etc in specific brain regions. I would like to recommend it to everybody who is interested in stereology. In addition, MBF provides excellent technical support and helps you to find the best technical tools for your research challenges on morphometry."
Wilma Van De Berg, Ph.D.
VU University Medical Center - Neuroscience Campus Amsterdam
"We've used Stereo Investigator for 10+ years. The technical support is outstanding, and the company itself it highly responsive to their customers."
Glenn Rosen, Ph.D.
Beth Israel Deaconess Medical Center
"If there is another program for unbiased stereology, I don't know what it is. This is the gold standard, and I can't say enough to praise the technical support provided by MBF Bioscience."
Bob Jacobs, Ph.D.