Stereo Investigator with Laser-Scanning Confocal Microscopes

Confocal laser-scanning microscopy is a powerful tool widely used by researchers to clearly visualize multiple labels and co-localization within a biological specimen. Stereo Investigator, in conjunction with the Olympus Fluoview®, is the only full-function laser-scanning confocal stereology system on the market. Together with Stereo Investigator, the gold standard for stereology, the Olympus Fluoview provides unparalleled power.

Stereology with Stereo Investigator

  • Stereo Investigator communicates directly with the Fluoview software for seamless operation
  • Fully integrate the implementation of Stereo Investigator’s effective design-based stereology protocols on a laser scanning confocal microscope system including:
  • Single-channel
  • Multi-channel
  • Attended with full control of PMTs and gain during image stack acquisition
  • Unattended with automatic acquisition from all sampling sites across multiple regions of interest, multiple sections, and multiple slides
  • Perform offline counting using independent workstations, freeing up valuable confocal time for other users
  • Acquire a permanent record of tissue samples of one to three channels (i.e. 3D multi-channel image stacks from each sampling site or multi-channel Virtual Slice montages depending on which stereology protocol is needed)
  • Estimate cell counts and fiber lengths in very thin tissue
  • Estimate cell counts in densely packed areas

Virtual Slice

  • Automatically collect exceptionally clean, clear, single- or multi-channel Virtual Slice images (2D) or Virtual Tissue data sets (3D) at high resolution across thousands of fields of view