Stereo Investigator Integration with the Olympus Fluoview

Confocal microscopy is a powerful tool widely used by researchers to clearly visualize multiple labels and co-localization within a biological specimen. The Olympus Fluoview® is the only full-function laser scanning confocal stereology option on the market. Together with Stereo Investigator®, the gold standard for stereology, the Olympus Fluoview provides unparalleled power.

Stereology with Stereo Investigator

• Stereo Investigator communicates directly with the Fluoview software for seamless operation

• Fully integrate the implementation of Stereo Investigator’s effective design-based stereology protocols on a laser scanning confocal microscope system including:

• Single-channel

• Multi-channel

• Attended with full control of PMTs and gain during image stack acquisition

• Unattended with automatic acquisition from all sampling sites across multiple regions of interest, multiple sections, and multiple slides

• Perform offline counting using independent workstations, freeing up valuable confocal time for other users

• Acquire a permanent record of tissue samples of one to three channels (i.e. 3D multi-channel image stacks from each sampling site or multi-channel Virtual Slice montages depending on which stereology protocol is needed)

• Estimate cell counts and fiber lengths in very thin tissue

• Estimate cell counts in densely packed areas

Virtual Slice

• Automatically collect exceptionally clean, clear, single- or multi-channel Virtual Slice images (2D) or Virtual Tissue data sets (3D) at high resolution across thousands of fields of view