Stereo Investigator Pulmonary Edition

Count alveoli and quantify cell types, areas, and volumes
using the world’s foremost system for stereology.


Stereo Investigator Pulmonary Edition introduces the Connectivity Assay for counting alveoli. Using live video superimposed over captured images, you can quickly mark bridges and islands.

Stereo Investigator Pulmonary
Edition is designed specifically
for pulmonary research

The Connectivity Assay Workflow: Using the ConnEuler probe, the workflow guides you step-by-step through the estimation process. Final displayed results can be transferred directly to Microsoft Excel.

Reliable Analysis: Stereo Investigator uses unbiased methods to produce reliable results. The software’s use of systematic random sampling conforms to the most rigorous stereological protocols.

Reduced Effort: Stereo Investigator pairs the most efficient methods with automated stage movement, sampling site placement, and easy-to-read graphical displays for rapid evaluation of your tissue. Stereo Investigator shortens the time between experiment and result.

Meets Your Research Needs: Stereo Investigator is compatible with all research microscopes—brightfield, fluorescent, or confocal. The software includes image analysis, mapping, graphical output and measurement tools all in one software package.

Most Widely Published: More scientific papers cite Stereo lnvestigator as the tool of choice than any other stereological analysis product. Stereo Investigator is used in hundreds of research labs throughout the world—a vital part of lung, cardio, kidney, and brain research.

Outstanding Support: MBF has been successfully providing our expertise, training, and support to researchers worldwide for 20 years. Our Live Remote support diagnoses problems remotely and keeps your system running properly.

Custom markers for bridges, holes and islands

Stereo Investigator Pulmonary Edition:

  • can analyze data from multiple modalities: using live images from digital or video cameras; stored image sets from confocal microscopes, electron microscopes, and scanning tomographic sources.
  • controls a motorized XYZ stage for integrated navigation through tissue sections, allowing for effortless measurement through many fields-of-view.
  • integrates unlimited sections into a single data file with the Serial Section Manager, maintaining each section in aligned 3D space for stereological analysis and systematic random sampling.
  • can analyze isotropic, vertical, or preferentially cut sections. The estimation results and confidence levels are calculated and displayed on demand, or can be exported.
  • also features sophisticated tools for anatomical mapping. These mapping tools can be used to delineate regions of interest for stereological estimators, to map cell distributions, to prepare anatomical maps for publication, and to perform detailed morphometric analyses.
  • offers the most comprehensive implementation of stereological probes and methods available. Plug-in modules are available for confocal and MRI analysis, 3D solid modeling, and virtual slide creation.


Nucleator estimator
determines cell volume

Features of Stereo Investigator Pulmonary Edition

  • Intuitive workflow technology, with the physical fractionator, to walk you through the process step-by-step
  • Easy-to-use physical fractionator and optical fractionator counting methods
  • Ability to count multiple cell types simultaneously
  • Availability of additional workstation licenses for offline processing
  • Automated guard zone support
  • In-bounds visual indicators in X, Y, and Z
  • Counting combined with local probes
  • Automated stage movement to speed data collection
  • Quality control and data review features
  • Complete editing capabilities
  • Individual and grouped results
  • Coefficient of Error (CE) and Variance (CV) calculations
  • Documentation of equations and formulae
  • Comprehensive user manual and HTML help
  • Integration of data from serial sections
  • Automatic serial section alignment tools
  • Automatic compensation for missing sections
  • Ability to switch between live and stored images
  • Flexible hardware support for all research microscopes
  • Support for Olympus Fluoview 300, 500, and 1000 confocal microscopes
  • Support for Olympus DSU and BD CARV II spinning disk confocals
  • Automated stack acquisition for offline analysis
  • Data calibrated to your microscope objective lenses
  • Automatic parcentric and parfocal correction
  • Image processing and morphometric analysis tools


Cavalieri estimator
determines tissue volume

Unbiased Stereological Estimators

  • Optical fractionator
  • ConnEuler probe/physical fractionator
  • Physical disector
  • Space Balls, isotropic virtual planes
  • Nucleator, planar rotator
  • Spatial distribution
  • Cavalieri estimator for area and volume

...plus more than 20 additional probes

For more information about Stereo Investigator, call MBF Bioscience at 802.288.9290 or click here to request a price quotation.