Spines and Synapses
Dendritic spines, the often bulbous protrusions that emerge from the dendritic shaft, are the main site of excitatory synapses in the brain .
Both dendritic spines and synapses can be detected and quantified using a Neurolucida system on a microscope, or image stacks. When working on a microscope, researchers can manually mark and classify dendritic spines along a traced dendrite, and obtain morphometric data about spines including length, number, and density.
The completely automatic option includes the following steps:
2. Save it to Biolucida to store and organize microscope images
3. Open the image stack with Neurolucida 360 (freeing up your microscope for another project)
4. Obtain accurate data and analysis for publication