Neurolucida - New in Version 9

NEW NEURON TOOLS
  • New tools to trace, classify and analyze dendritic spines
  • New morphometric analyses for neuronal analysis
  • Improved editing features for easier tree repair

AUTOMATIC CONTOURING
  • Let the software automatically trace anatomical regions and section boundaries from images, image stacks, virtual slides, and even off the live camera feed
  • If you need to move the stage, the software does it automatically and continues tracing beyond the field-of-view

AUTOMATIC OBJECT DETECTION
  • Let the software find cells, synapses, grains, or similar types of objects that interest you in your images
  • Mark and count the objects or have Neurolucida automatically trace around the outside of an object

MULTIPLE USER PROFILES
  • Create configurations for different users and groups in your lab, or organize by experiment type
  • Use a single profile for all users, or use any number of unique profiles with customized settings.
  • Switch between multiple hardware setups with separate settings

IMAGE HANDLING
  • Work with high-bit depth, n-channel (up to 16 bits per channel) images via a flexible and intuitive user interface
  • Acquire up to six independent channels of high-bit depth data per image from any of our cameras
  • Work with the latest image file formats from the major confocal microscope manufacturers
  • Load, view, and analyze multiple image stacks at once
  • Enhanced ability to work with 3D virtual slide stacks include saving portions of these large montage images (and stacks) to separate files
  • New image display control mechanism provides precise histogram adjustments for enhanced visualization
  • Preserve original acquired image data after modifications including acquisition adjustments post-acquisition

IMPROVED 3D VISUALIZATION
  • A fully revamped 3D visualization window makes it easier to view, manipulate, and understand image stacks and tracings
  • View cut planes in the X, Y, and Z axis from any angle
  • Manipulate the transparency to get the look you want from your combined image and tracing data
  • New automatic rotation tool that lets you adjust the angle of rotation and the speed

64-BIT / WINDOWS 7 COMPATIBLE
  • Load and work with extremely large images and image stacks with 64-bit support
  • Take advantage of the performance and reliability of Microsoft Windows 7
  • New monochrome and color cameras for 64-bit Vista and 64-bit Windows 7

JPEG 2000 IMAGE FILE SUPPORT
  • Dramatically reduce disk space usage for all your image data
  • Image stacks that were 100 MB stored in a traditional TIFF file shrink to about 5 MB
  • Lossless compression to reconstruct the original image data from a compressed file
  • Ultra fast loading and viewing of image stacks
  • Create large virtual slices that exceed the computer’s physical memory and store these files using manageable file sizes

HARDWARE SUPPORT
  • Swap multiple cameras on the fly with an improved camera interface
  • Support for Zeiss® AxioCam®, QImaging®, and Baumer® cameras with hot switch ability
  • Comprehensive integration with the Zeiss ApoTome® structured illumination device
  • Support for the Qioptic Optigrid® structured illumination device

SIMPLIFIED MENUS AND HOT KEYS
  • Streamlined workflows with more intuitive menus and dialogs
  • New navigation keys help you maneuver around with less effort

SERIAL SECTIONS
  • Combine files together into a complete data file with fewer steps and a more intuitive interface
  • Now use the Serial Section Manager to define many sections in one step—and view multiple and flanking sections

ADDITIONAL FEATURES
  • Adjust the transparency of the tracing to see the underlying image while tracing
  • Drag and drop your images, image stacks, and data files for easier loading and display
  • Support for Aperio® and Hamamatsu® Nanozoomer® virtual slide files
  • Enhanced Help integration

AutoNeuron – New in Version 4

INTERACTIVE MODE
  • New Workflow
  • Interactive tracing: Dynamic point-to-end-point display of the proposed tracing path while moving the mouse
  • Easily specify intermediate tracing points. Adding a new branch is as simple as a single click

REGIONS OF INTEREST
  • Identify one or more regions of interest to include or exclude, creating an automatic neuron tracing zone

EDITING TOOLS
  • Improved editing tools, including quick splice and quick-detach tools for correcting tracing errors

SEED PLACEMENT
  • Use distinct colors to differentiate between automatically- and manually-placed seeds automatic tracing

BLENDED CHANNELS
  • Create combined reconstructions of multiple neurons in separate color channels