Preferences: Spines
Access spine preferences from File > Preferences > Tracing > Spines
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Display Style: Modify colors using the color pickers and shape using the drop-down menus (hollow or filled circle).
Click Reset to revert to system defaults
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Show spine volume if available: Check or clear the checkbox to display/hide spine volumes if they are available. When checked, the volumes of spine heads are calculated.
Choose your preferred style for default spine-volume display in the 2D window by clicking one of the radio buttons.
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Shaft Style: Select your preferred style from the drop-down menu.
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Anchor Point
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Use closest branch point: Select to attach the spine to the branch point nearest to where you clicked.
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Insert new connection point if no existing point within this distance: Inserts a new branch point if needed.
 
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One-shot spine placement: Returns to the manual Tracing mode automatically (i.e., the cursor blinks at the most recent branch point) upon placing one spine.