Save /Save as

Procedure and Commands

  1. Go to File >

    • Save if you want to save updates to a file that has been previously saved and you do not want to update the file name or location. This will overwrite the original file.

    • Save As if you're saving a file for the first time or want to save a file with an updated name or location. This will not change the original file.

  2. Click the icon that corresponds to the type of file you want to save.

    Note that the list below includes commands found in both Save and Save As

Image: Save an image.
Image stack: Save multiplane images—images that include multiple Z-planes .

Current Plane: Save only the currently displayed plane in an image stack (multiplane image) .

  • (optional) Save as type drop-down menu:

    • MBF JPEG2000 is the default file type: we recommend it.

    • MBF JPEG2000 and MBF TIFF files can include high bit-depth pixel data (if present) and image-display adjustments you've made in BrightSLICE. Metadata about the image, such as the microscope hardware used, is also saved.

    • JPEG2000 file format offers smart compression that can result in a significant reduction in the amount of storage space needed, with minimal data loss.

    • TIFF file format is uncompressed.

  • Export the image file: Check the box to save (export) the image and break its link to any associated data file.

    • The original image-display information and any high bit depth data that were present are lost.

    • This enables selection of additional Image Detail options or Stack Save Options (described below).

    • When checked, Save changes to Export.

  • Image Detail options:

    • Save as individual channels [available for multiple color-channel images]: Saves each color-channel in the image as a separate file.

      The channel number is added to the end of the file name, e.g., "FileName_Ch01.jp2"

    • Save image color [available when you select Save as individual channels]: Saves the color information with each separate channel or stack.

  • Stack Save Options:

    • Same as Image details options, plus:

    • Save as a series of single image files: Save each image plane as a separate file.

      The plane is added to the end of the file name, e.g., "FileName_001.jp2" or "FileName_001_Ch01.jp2"

  • Compression (available when either JPEG2000 file type is selected): Use the slider to indicate the amount of file compression desired.