Researchers cited MBF Bioscience systems in 19 papers between 7/27/2018 and 8/3/2018

Stereo Investigator:

Bassey, R. B., & Gondré-Lewis, M. C. (2018). Combined early life stressors: Prenatal nicotine and maternal deprivation interact to influence affective and drug seeking behavioral phenotypes in rats. Behavioural Brain Research. doi:

Deniz, Ö. G., Altun, G., Kaplan, A. A., Yurt, K. K., von Bartheld, C. S., & Kaplan, S. (2018). A concise review of optical, physical and isotropic fractionator techniques in neuroscience studies, including recent developments. Journal of Neuroscience Methods. doi:

Milanese, C., Cerri, S., Ulusoy, A., Gornati, S. V., Plat, A., Gabriels, S., . . . Mastroberardino, P. G. (2018). Activation of the DNA damage response in vivo in synucleinopathy models of Parkinson’s disease. Cell death & disease, 9(8), 818. doi: 10.1038/s41419-018-0848-7.

Pozner, T., Vistoropsky, Y., Moaraf, S., Heiblum, R., & Barnea, A. (2018). Questioning Seasonality of Neuronal Plasticity in the Adult Avian Brain. Scientific Reports, 8(1), 11289. doi: 10.1038/s41598-018-29532-1.  Continue reading “Researchers cited MBF Bioscience systems in 19 papers between 7/27/2018 and 8/3/2018” »

Researchers cited MBF Bioscience systems in 26 papers between 7/13/2018 and 7/27/2018

Stereo Investigator:

Eriksson, Y., Boström, M., Sandelius, Å., Blennow, K., Zetterberg, H., Kuhn, G., & Kalm, M. (2018). The anti-asthmatic drug, montelukast, modifies the neurogenic potential in the young healthy and irradiated brain. Cell death & disease, 9(7), 775. doi: 10.1038/s41419-018-0783-7.

Kim, D., Yoo, J. M., Hwang, H., Lee, J., Lee, S. H., Yun, S. P., . . . Ko, H. S. (2018). Graphene quantum dots prevent α-synucleinopathy in Parkinson’s disease. Nature Nanotechnology. doi: 10.1038/s41565-018-0179-y.

Olivia, P., J., C. L., Pamela, B. L., G., A. D., & Pierre, L. (2018). Stereological analysis of the rhesus monkey entorhinal cortex. Journal of Comparative Neurology, 0(ja). doi:10.1002/cne.24496.

Pooley, A. E., Benjamin, R. C., Sreedhar, S., Eagle, A. L., Robison, A. J., Mazei-Robison, M. S., . . . Jordan, C. L. (2018). Sex differences in the traumatic stress response: the role of adult gonadal hormones. Biology of Sex Differences, 9(1), 32. doi: 10.1186/s13293-018-0192-8. Continue reading “Researchers cited MBF Bioscience systems in 26 papers between 7/13/2018 and 7/27/2018” »

Researchers cited MBF Bioscience systems in 8 papers between 7/6/2018 and 7/13/2018

Stereo Investigator:

Bok, E., Chung, Y. C., Kim, K.-S., Baik, H. H., Shin, W.-H., & Jin, B. K. (2018). Modulation of M1/M2 polarization by capsaicin contributes to the survival of dopaminergic neurons in the lipopolysaccharide-lesioned substantia nigra in vivo. Experimental and Molecular Medicine, 50(7), 76. doi: 10.1038/s12276-018-0111-4.

Li, S., Yip, A., Bird, J., Soo Seok, B., Chan, A., Godden, K. E., . . . Pompeiano, M. (2018). Melanin-concentrating hormone (MCH) neurons in the developing chick brain. Brain Research. doi:

Pereira, R., Leite, E., Raimundo, J., Guilherme, S., Puga, S., Santos, M. A., . . . Pereira, P. (2018). Metals(loids) targeting fish eyes and brain in a contaminated estuary – Uncovering neurosensory (un)susceptibility through bioaccumulation, antioxidant and morphometric profiles. Marine Environmental Research. doi:

Pooley, A. E., Benjamin, R. C., Sreedhar, S., Eagle, A. L., Robison, A. J., Mazei-Robison, M. S., . . . Jordan, C. L. (2018). Sex differences in the traumatic stress response: PTSD symptoms in women recapitulated in female rats. Biology of Sex Differences, 9(1), 31. doi: 10.1186/s13293-018-0191-9.  Continue reading “Researchers cited MBF Bioscience systems in 8 papers between 7/6/2018 and 7/13/2018” »

Researchers cited MBF Bioscience systems in 13 papers between 6/29/2018 and 7/6/2018

Stereo Investigator:

Aguilar-Valles, A., Haji, N., De Gregorio, D., Matta-Camacho, E., Eslamizade, M. J., Popic, J., . . . Sonenberg, N. (2018). Translational control of depression-like behavior via phosphorylation of eukaryotic translation initiation factor 4E. Nature Communications, 9(1), 2459. doi: 10.1038/s41467-018-04883-5.

Bearer, E. L., Manifold-Wheeler, B. C., Medina, C. S., Gonzales, A., Cháves, F., & Jacobs, R. E. (2018). Alterations of functional circuitry in aging brain and the impact of mutated APP expression. Neurobiology of Aging. doi:

Holahan, M. R., Smith, C. A., Luu, B. E., & Storey, K. B. (2018). Preadolescent phthalate (DEHP) exposure is associated with elevated locomotor activity and reward-related behavior and a reduced number of tyrosine hydroxylase positive neurons in post-adolescent male and female rats. Toxicological Sciences, kfy171-kfy171. doi: 10.1093/toxsci/kfy171.

Li, Y., Stockton, M. E., Eisinger, B. E., Zhao, Y., Miller, J. L., Bhuiyan, I., . . . Zhao, X. (2018). Reducing histone acetylation rescues cognitive deficits in a mouse model of Fragile X syndrome. Nature Communications, 9(1), 2494. doi: 10.1038/s41467-018-04869-3.  Continue reading “Researchers cited MBF Bioscience systems in 13 papers between 6/29/2018 and 7/6/2018” »

Researchers cited MBF Bioscience systems in 23 papers between 6/8/2018 and 6/22/2018

Stereo Investigator:

Caracciolo, L., Marosi, M., Mazzitelli, J., Latifi, S., Sano, Y., Galvan, L., . . . Carmichael, S. T. (2018). CREB controls cortical circuit plasticity and functional recovery after stroke. Nature Communications, 9(1), 2250. doi: 10.1038/s41467-018-04445-9.

Darling, W. G., Ge, J., Stilwell-Morecraft, K. S., Rotella, D. L., Pizzimenti, M. A., & Morecraft, R. J. (2018). Hand Motor Recovery following Extensive Frontoparietal Cortical Injury is accompanied by Upregulated Corticoreticular Projections in the Monkey. The Journal of Neuroscience. doi:

George, P. M., Oh, B., Dewi, R., Hua, T., Cai, L., Levinson, A., . . . Steinberg, G. K. (2018). Engineered stem cell mimics to enhance stroke recovery. Biomaterials, 178, 63-72. doi:

Giza, J. I., Kim, J., Meyer, H. C., Anastasia, A., Dincheva, I., Zheng, C. I., . . . Lee, F. S. (2018). The BDNF Val66Met Prodomain Disassembles Dendritic Spines Altering Fear Extinction Circuitry and Behavior. Neuron. doi:

LaPlaca, M. C., Lessing, M. C., Prado, G. R., Zhou, R., Tate, C. C., Geddes-Klein, D., . . . Zhang, L. (2018). Mechanoporation is a potential indicator of tissue strain and subsequent degeneration following experimental traumatic brain injury. Clinical Biomechanics. doi:  Continue reading “Researchers cited MBF Bioscience systems in 23 papers between 6/8/2018 and 6/22/2018” »

Researchers cited MBF Bioscience systems in 10 papers between 6/1/2018 and 6/8/2018


Stereo Investigator:

Aravanis, C. V., Kapelouzou, A., Vagios, S., Tsilimigras, D. I., Katsimpoulas, M., Moris, D., . . . Liakakos, T. (2018). Toll-Like Receptors -2, -3, -4 and -7 Expression Patterns in the Liver of a CLP-Induced Sepsis Mouse Model. Journal of Investigative Surgery, 1-9. doi: 10.1080/08941939.2018.1476630.

Cerqueira, S. R., Lee, Y.-S., Cornelison, R. C., Mertz, M. W., Wachs, R. A., Schmidt, C. E., & Bunge, M. B. (2018). Decellularized peripheral nerve supports Schwann cell transplants and axon growth following spinal cord injury. Biomaterials. doi:

Karlsson, L., González-Alvarado, M. N., Larrosa-Flor, M., Osman, A., Börjesson, M., Blomgren, K., & Kuhn, H. G. (2018). Constitutive PGC-1α Overexpression in Skeletal Muscle Does Not Improve Morphological Outcome in Mouse Models of Brain Irradiation or Cortical Stroke. Neuroscience. doi:

Kukreja, L., Shahidehpour, R., Kim, G., Keegan, J., Sadleir, K. R., Russell, T., . . . Geula, C. (2018). Differential Neurotoxicity Related to Tetracycline Transactivator and TDP-43 Expression in Conditional TDP-43 Mouse Model of Frontotemporal Lobar Degeneration. [10.1523/JNEUROSCI.1836-17.2018]. The Journal of Neuroscience. doi: Continue reading “Researchers cited MBF Bioscience systems in 10 papers between 6/1/2018 and 6/8/2018” »

Researchers cited MBF Bioscience systems in 8 papers between 5/14/2018 and 5/25/2018

Stereo Investigator:

Bird, C. W., Taylor, D. H., Pinkowski, N. J., Chavez, G. J., & Valenzuela, C. F. (2018). Long-term Reductions in the Population of GABAergic Interneurons in the Mouse Hippocampus following Developmental Ethanol Exposure. Neuroscience, 383, 60-73. doi:

Harun-Or-Rashid, M., Pappenhagen, N., Palmer, P. G., Smith, M. A., Gevorgyan, V., Wilson, G. N., . . . Inman, D. M. (2018). Structural and functional rescue of chronic metabolically stressed optic nerves through respiration. [10.1523/JNEUROSCI.3652-17.2018]. The Journal of Neuroscience. DOI:

Obara-Michlewska, M., Ding, F., Popek, M., Verkhratsky, A., Nedergaard, M., Zielinska, M., & Albrecht, J. (2018). Interstitial ion homeostasis and acid-base balance are maintained in oedematous brain of mice with acute toxic liver failure. Neurochemistry International. doi: Continue reading “Researchers cited MBF Bioscience systems in 8 papers between 5/14/2018 and 5/25/2018” »

Diet Restriction Slows Neurodegeneration and Extends Lifespan of DNA-Repair-Deficient Mice

DNA damage occurs in human cells at a constant rate. These cells are usually able to repair themselves, but sometimes deficiencies in certain genes cause the repair process to shut down. When damaged DNA isn’t fixed, mutations can occur that cause accelerated aging or cancerous tumors to form (Hoeijmakers, 2009). Scientists at Erasmus University Medical Center in Rotterdam have found a way to slow down the process – at least in mice.

In a study published in Nature, the researchers report that when mice deficient in the DNA-repair genes Ercc1 or Xpg are put on a restricted diet, they experience better overall health and increased lifespans compared to DNA-repair-deficient mice fed a normal diet. They also found significantly lower levels of neurodegeneration in the brains and spinal cords of diet restricted animals compared to controls.

“Here we report that a dietary restriction of 30 percent tripled the median and maximal remaining lifespans of these progeroid mice, strongly retarding numerous aspects of accelerated aging Mice undergoing dietary restriction retained 50 percent more neurons and maintained full motor function far beyond the lifespan of mice fed ad libitum,” (Vermeij, et al 2016).

Since the DNA-repair-deficient mice were already smaller and weaker than normal mice, the Rotterdam researchers wondered whether diet restriction would be beneficial or detrimental to their health. They found that gradually restricting the diets of DNA-repair-deficient mice starting at age seven weeks increased their median lifespans from 10 to 35 weeks in males and 13 to 39 weeks in females as compared to controls.

They also saw significant differences in the levels of neurodegeneration between these two populations. Using Stereo Investigator, they found 50 percent more neurons in the brains of diet-restricted mice compared to those fed a normal diet. They also saw lower levels of cells expressing p53 – a protein expressed in response to DNA damage – in diet-restricted mice.

According to the authors, dietary restriction may not fix defects in DNA repair mechanisms, but it may help to reduce the severity and speed at which the damage occurs.

“Our findings establish the Ercc1 mouse as a powerful model organism for health-sustaining interventions, reveal potential for reducing endogenous DNA damage, facilitate a better understanding of the molecular mechanism of dietary restriction and suggest a role for counterintuitive dietary-restriction-like therapy for human progeroid genome instability syndromes and possibly neurodegeneration in general,” (Vermeij, et al 2016).

Vermeij W.P., Dollé M.E.T., Reiling E., Jaarsma D., Payan-Gomez C, Bombardieri C.R., Wu H., Roks A.J.M., Botter S.M., van der Eerden B.C., Youssef S.A., Kuiper R.V., Nagarajah B., van Oostrom C.T., Brandt R.M.C., Barnhoorn S., Imholz S., Pennings J.L.A., de Bruin A., Gyenis Á., Pothof J, Vijg J, van Steeg H., and Hoeijmakers J.H.J. (2016) Restricted diet delays accelerated aging and genomic stress in DNA repair deficient mice. Nature 537, 427-431, doi:10.1038/nature19329

Hoeijmakers JH (2009) DNA Damage, aging, and cancer. N Engl J Med; 361:1475-1485, DOI: 10.1056/NEJMra0804615

Stock image of DNA used in accordance with the CC0 public domain license.

Uncovering the role of microglia in fetal alcohol spectrum disorders


Representative images of Iba-1+ microglia in the postnatal day 10 rat hippocampus. Image courtesy of Anna Klintsova, PhD.

Children born with fetal alcohol spectrum disorders face a range of physical and cognitive impairments including long-term deficits in learning, behavior, and immune function. In a paper published in Neuroscience, Dr. Anna Klintsova and her lab at the University of Delaware report that activation of the brain’s immune response may contribute to some of the damage caused by fetal alcohol spectrum disorders.

In their study, the researchers used Stereo Investigator and Neurolucida to examine the hypothesis that exposure to alcohol while the brain is growing rapidly is associated with abnormal microglial activation and high levels of pro-inflammatory proteins which impair learning-related plasticity; leading to neuro-developmental and psychopathological disorders.

“My lab has been using both Stereo Investigator and Neurolucida for more than a decade in all quantitative neuroanatomical studies, including the featured one,” said Dr. Anna Klintsova. “We find this software to be user-friendly, reliable and essential for obtaining unbiased results.”

They used Stereo Investigator to quantify the number of microglia in the hippocampus of neonatal rats who were exposed to alcohol during the equivalent of the third trimester of a human pregnancy. The researchers expected to see an increased number of microglia in alcohol-exposed neonatal rats, however they found a decreased number of microglia. Despite the decrease in microglia number, there was a significant increase in pro-inflammatory proteins expressed by microglia and an increase in microglial activation.

To measure microglial activation, the researchers quantified the area of cell territory using Neurolucida. Activated microglia have a smaller cell territory than resting microglia, so the smaller cell territory found in alcohol exposed rats indicates a more active state.

This research supports the hypothesis that abnormal microglia activation plays a role in fetal alcohol spectrum disorders, however more research is needed to further understand the relationship.

Boschen, K., Ruggiero, M.J., Klintsova, A.Y., (2016) Neonatal binge alcohol exposure increases microglial activation in the developing rat hippocampus. Neuroscience 324: 355–366. DOI: 10.1016/j.neuroscience.2016.03.033


Stereological Study Reveals Neuron and Glia Proliferation in Hippocampus of Lithium-Treated Mice

Dentate gyruspilot

The optical fractionator probe was used to quantify the number of neurons and glia in the dentate gyrus

Doctors have used lithium to treat patients with bipolar disorder since the 1970s. Known for its efficacy in stabilizing patients’ moods by regulating manic episodes, lithium is also associated with a decreased risk of suicide. But while this naturally occurring element is the most widely prescribed medication for those suffering from bipolar disorder, scientists still have much to learn about how lithium physically affects the brain.

A recent study published in the journal Bipolar Disorders adds to the growing body of evidence that says lithium contributes to cell proliferation in parts of the brain. Conducted by scientists at the University of Mississippi and the VU University Medical Center in Amsterdam, the study revealed an increased number of neurons and glia, and increased astrocyte density in the dentate gyrus of lithium-treated mice versus controls treated with a placebo.

Using the optical fractionator probe in Stereo Investigator, the researchers quantified the number of Nissl stained neurons and glial cells, and calculated astrocyte density. The results showed twenty-five percent more neurons and twenty-one percent more glia in the denate gyrus of lithium-treated mice. They also performed a stereological examination of another brain region – the medial prefrontal cortex (mPFC), but did not witness significant differences between lithium-treated and control mice in this area.

“In this study, particular cortical regions, ie. the fascia dentata in the hippocampus and the mPFC in the cerebral cortex needed to be selected in histological sections of the mice brains,” explained Dr. Harry B.M. Uylings, “therefore the stereological counting procedure applied was the best one. Stereo Investigator greatly assisted in the counting of cells, and the software’s excel data-output was especially beneficial.”

According to the paper, the findings present a more detailed picture of lithium-induced alterations in the dentate gyrus cellular phenotype than previously available, and provide the first evidence for lithium-induced increases in glia and astrocytes.

The authors also explain that while cell number increased in the dentate gyrus of lithium-treated mice, the region’s overall volume as well as that of the greater hippocampus was unaffected by the element. The volume of the dentate gyrus and the hippocampus as a whole was measured with the Cavalieri method in Stereo Investigator.  The researchers describe the dissociation between cell proliferation and volume as “an interesting observation that warrants further investigation.”

Rajkowska, G., Clarke, G., Mahajan, G., Licht, C.M., van de Werd, H.J., Yuan, P., Stockmeier, C.A., Maji, H.K., Uylings, H.B., Differential effect of lithium on cell number in the hippocampus and prefrontal cortex in adult mice: a stereological study. Bipolar Disord. 2016 Feb;18(1):41-51. doi: 10.1111/bdi.12364.