Researchers cited MBF systems in 7 papers during the week of 12/02/13

Stereo Investigator:journal images sm

Researchers cited MBF systems in 25 papers during the week of 11/25/13

Stereo Investigator:journal images sm

Our systems were cited in 31 published research papers last week. (week of 09/16/13)

Take a closer look at some of the research…

Stereo Investigator:bookshelves-at-the-library_w482_h725

Aumann, T. D., Tomas, D., & Horne, M. K. (2013). Environmental and behavioral modulation of the number of substantia nigra dopamine neurons in adult mice. Brain and Behavior, n/a-n/a. doi: 10.1002/brb3.163.
http://dx.doi.org/10.1002/brb3.163

Becknell, B., Carpenter, A. R., Allen, J. L., Wilhide, M. E., Ingraham, S. E., Hains, D. S., & McHugh, K. M. (2013). Molecular Basis of Renal Adaptation in a Murine Model of Congenital Obstructive Nephropathy. Plos one, 8(9), e72762. doi: 10.1371/journal.pone.0072762.
http://dx.doi.org/10.1371%2Fjournal.pone.0072762

Bissonnette, S., Vaillancourt, M., Hébert, S. S., Drolet, G., & Samadi, P. (2013). Striatal Pre-Enkephalin Overexpression Improves Huntington’s Disease Symptoms in the R6/2 Mouse Model of Huntington’s Disease. Plos one, 8(9), e75099. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770591/

Gray, K. (2013). Society for Pediatric Pathology, 2013 Fall Meeting Abstracts. Pediatric and Developmental Pathology. doi: 10.2350/13-09-1379-oa.1.
http://dx.doi.org/10.2350/13-09-1379-OA.1

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Take a look at the latest research using MBF systems (week of 8/26/13)

Our systems were mentioned in 23 published research papers last week. Take a closer look at some of the research…bookshelves-at-the-library_w482_h725

Stereo Investigator:

Campeau, L., Soler, R., Sittadjody, S., Pareta, R., Nomiya, M., Zarifpour, M., . . . Andersson, K. E. (2013). Effects of allogeneic bone marrow-derived mesenchymal stromal cell therapy on voiding function in a rat model of Parkinson’s disease. The Journal of urology(0). doi: http://dx.doi.org/10.1016/j.juro.2013.08.026.

Doorn, K. J., Goudriaan, A., Blits-Huizinga, C., Bol, J. G. J. M., Rozemuller, A. J., Hoogland, P. V. J. M., . . . van Dam, A.-M. (2013). Increased Amoeboid Microglial Density in the Olfactory Bulb of Parkinson’s and Alzheimer’s Patients. Brain Pathology, n/a-n/a. doi: 10.1111/bpa.12088. http://dx.doi.org/10.1111/bpa.12088

Fard, M. A., Ebrahimi, K. B., & Miller, N. R. (2013). RhoA activity and post-ischemic inflammation in an experimental model of adult rodent anterior ischemic optic neuropathy. Brain Research(0). doi:http://dx.doi.org/10.1016/j.brainres.2013.07.053.

Kiessling, M. C., Büttner, A., Butti, C., Müller-Starck, J., Milz, S., Hof, P. R., . . . Schmitz, C. (2013). Intact Numbers of Cerebellar Purkinje and Granule Cells in Sudden Infant Death Syndrome: A Stereologic Analysis and Critical Review of Neuropathologic Evidence. Journal of Neuropathology and Experimental Neurology.http://europepmc.org/abstract/MED/23965745/reload=0;jsessionid=KfJ2QuIXQ…

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Boston University Scientists Use AutoNeuron and AutoSynapse to Compare Neurons in the Visual and Prefrontal Cortices

MBF_Figure

Use of Neurolucida to assess the detailed morphology – including spines and synapses – of a layer 3 pyramidal neuron from the anterior cingulate cortex of a rhesus monkey. A) 40x confocal image of a layer 3 pyramidal neuron that was filled with biocytin during whole-cell patch-clamp recordings and subsequently processed with Alexa-Streptavidin-488. B) Basilar dendritic segment (indicated by the box in A), scanned in 2 channels: green= the neuron; red= VGat. C) Dendritic reconstruction indicated in light blue. Spine subtypes identified by the AutoSpine module (magenta= mushroom; yellow= thin; red= stubby). D) VGat-positive appositions (indicated by white dots) against the dendritic shaft and spines identified with AutoSynapse. Scale bars: A= 20 µm; B= 2 µm

The ball comes flying and you swing the bat. A car pulls out and you hit the brakes. As we go about our daily routines, we process everything we see in a region in the back of our brains known as the visual cortex. But when we sit down to plan a kitchen remodel, or next summer’s vacation, an area in the front of the brain gets activated, the prefrontal cortex, a region involved in higher level thinking.

Recent research has offered insight into the structure and function of neurons in these two distinct brain regions. Scientists at the Luebke Lab at Boston University set out to find out more about their morphology, and if their structural differences affect their behavior. Their study, published in the Journal of Neuroscience offers evidence that pyramidal neurons in the primary visual cortex (V1) and dorsolateral granular prefrontal cortex (dlPFC) of the rhesus monkey display “marked electrophysiological and structural differences.”

“We chose to examine these two areas because they represent distinct ends of the spectrum of neocortical complexity and specialization, from primary sensory processing by V1 to mediation of high-order cognitive processes by dlPFC,” the authors say in their paper.

Continue reading “Boston University Scientists Use AutoNeuron and AutoSynapse to Compare Neurons in the Visual and Prefrontal Cortices” »

Watch April’s AutoNeuron Webinar On Our Website

A great way to get better acquainted with MBF Bioscience’s software is to take one of our webinars. Hosted by Dr. Susan Hendricks, the online presentations demonstrate in real time how you can get the most out of our products.

Our latest webinar “Automated Neuron Reconstruction with AutoNeuron” is now available at mbfbioscience.com. So if you missed last month’s webinar, or if you’d like a refresher, you can access the recording any time you’d like, and view the entire hour-long presentation at your convenience.

Listen as Dr. Hendricks explains how to use the AutoNeuron extension module for Neurolucida to automatically reconstruct a labeled neuron. A series of slides provides the visuals as she uses the workflow to create a 3D model of the cell. Automatic soma detection, automatic and interactive branch reconstruction, and 3D display of the image data and the trace model are all discussed.

To find out about upcoming webinars, fan us on Facebook and follow us on Twitter.

Faster and More Powerful–AutoNeuron 4 Delivers

MBF Bioscience AutoNeuron

With the release of Neurolucida 9 comes version 4 of AutoNeuron. At MBF Bioscience our customers’ suggestions often become our newest features. What’s new and improved?

Windows 7/64-Bit: We’ve tested—and passed—Neurolucida and AutoNeuron on both 32-bit and 64-bit versions of Windows 7. The 64-bit version allows more memory and bigger data sets.

Interactive Mode: We’ve put a lot of work into making this easier to use. AutoNeuron now offers dynamic point-to-end-point display of the proposed tracing path while the user moves the mouse. Easily specify intermediate tracing points, too. Adding a new branch is as simple as a single click.

Regions of Interest: Identify one or more regions of interest for automatic tracing using contours, and specify the upper and lower Z limits for tracing. This makes it much easier and faster to limit tracing to just a portion of your 2D image or 3D image stack.

Editing Tools: Automated tracing can produce errors quite different from those seen with manual tracing. Our improved editing tools, which now include quick-splice and quick-detach tools for correcting tracing errors, make correcting errors easy.

Seed Placement: Apply distinct colors to differentiate between automatically- and manually-placed seeds used in automatic tracing. When you clear all seeds, choose to keep your manually placed seeds after the
refresh.

Blended Channels: In previous versions, AutoNeuron could trace independent confocal channels. With our new release, you can trace selectively combined channels as well.

“AutoNeuron is the future of quantitative neuromorphology. I am particularly excited about AutoNeuron’s interactive function because it will allow for much more efficient tracing of Golgi stained neurons, which are notoriously difficult to quantify. Cajal would be proud!” – Bob Jacobs, PhD, Thomas M. McKee Professor in the Natural Sciences, Department of Psychology; Co-Director of Neuroscience, The Colorado College

Download Neurolucida 9 with AutoNeuron 4 at www.mbfbioscience.com.

First published in The Scope, fall 2009.